Granulocytic Ehrlichiosis in the Laboratory Mouse
C3H mice that were inoculated with ehrlichiae isolated from a patient with human granulocytic ehrlichiosis (HGE) developed anemia and leukopenia, but by day 24, they returned to normal values. Granulocytic morulae were present in peripheral blood and spleen smears on days 5 and 10, and there was a r...
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| Veröffentlicht in: | The Journal of infectious diseases 1998-03, Vol.177 (3), p.737-745 |
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| container_title | The Journal of infectious diseases |
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| creator | Hodzic, Emir IJdo, Jacob W. I. Feng, Sunlian Katavolos, Paula Sun, Wei Maretzki, Craig H Fish, Durland Fikrig, Erol Telford, Sam R. Barthold, Stephen W. |
| description | C3H mice that were inoculated with ehrlichiae isolated from a patient with human granulocytic ehrlichiosis (HGE) developed anemia and leukopenia, but by day 24, they returned to normal values. Granulocytic morulae were present in peripheral blood and spleen smears on days 5 and 10, and there was a reduction in morulae on day 17. Ehrlichiae were present in HL-60 cell cultures of blood and spleen from all mice at all intervals. Pathogenicity, but not infectivity, waned with mouse passage but could be resurrected by SCID mouse passage. Various methods were tested for their relative sensitivity in detecting infection: blood smears, HL-60 cell cultures, polymerase chain reaction (PCR) amplification of a 16S recombinant DNA target, and a mouse infectivity assay. All assays detected the HGE agent in blood during early infection, but PCR and the mouse infectivity assay were most sensitive during late infection. Xenodiagnosis demonstrated that mice remain persistently infected through 55 days. |
| doi_str_mv | 10.1086/514236 |
| format | Article |
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Various methods were tested for their relative sensitivity in detecting infection: blood smears, HL-60 cell cultures, polymerase chain reaction (PCR) amplification of a 16S recombinant DNA target, and a mouse infectivity assay. All assays detected the HGE agent in blood during early infection, but PCR and the mouse infectivity assay were most sensitive during late infection. Xenodiagnosis demonstrated that mice remain persistently infected through 55 days.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/514236</identifier><identifier>PMID: 9498456</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Age Factors ; Anaplasma phagocytophilum ; Animals ; Bacterial diseases ; Biological and medical sciences ; Blood ; Disease Models, Animal ; Disease Susceptibility ; Disease Vectors ; Ehrlichia - pathogenicity ; Ehrlichiosis ; Ehrlichiosis - etiology ; Ehrlichiosis - immunology ; Experimental bacterial diseases and models ; Granulocytes ; Granulocytes - microbiology ; Humans ; Infections ; Infectious diseases ; Inoculation ; Ixodes - microbiology ; Leukocyte Count ; Major Articles ; Medical sciences ; Mice ; Mice, Inbred C3H ; Mice, SCID ; Spleen ; Spleen - pathology ; Ticks</subject><ispartof>The Journal of infectious diseases, 1998-03, Vol.177 (3), p.737-745</ispartof><rights>Copyright 1998 The University of Chicago</rights><rights>1998 INIST-CNRS</rights><rights>Copyright University of Chicago, acting through its Press Mar 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-68b169bfc18da7791386cbc0c9fce0eb91fea3f2a73e81aebce870f1b65641e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30106924$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30106924$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2174913$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9498456$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hodzic, Emir</creatorcontrib><creatorcontrib>IJdo, Jacob W. I.</creatorcontrib><creatorcontrib>Feng, Sunlian</creatorcontrib><creatorcontrib>Katavolos, Paula</creatorcontrib><creatorcontrib>Sun, Wei</creatorcontrib><creatorcontrib>Maretzki, Craig H</creatorcontrib><creatorcontrib>Fish, Durland</creatorcontrib><creatorcontrib>Fikrig, Erol</creatorcontrib><creatorcontrib>Telford, Sam R.</creatorcontrib><creatorcontrib>Barthold, Stephen W.</creatorcontrib><title>Granulocytic Ehrlichiosis in the Laboratory Mouse</title><title>The Journal of infectious diseases</title><addtitle>The Journal of Infectious Diseases</addtitle><description>C3H mice that were inoculated with ehrlichiae isolated from a patient with human granulocytic ehrlichiosis (HGE) developed anemia and leukopenia, but by day 24, they returned to normal values. Granulocytic morulae were present in peripheral blood and spleen smears on days 5 and 10, and there was a reduction in morulae on day 17. Ehrlichiae were present in HL-60 cell cultures of blood and spleen from all mice at all intervals. Pathogenicity, but not infectivity, waned with mouse passage but could be resurrected by SCID mouse passage. Various methods were tested for their relative sensitivity in detecting infection: blood smears, HL-60 cell cultures, polymerase chain reaction (PCR) amplification of a 16S recombinant DNA target, and a mouse infectivity assay. All assays detected the HGE agent in blood during early infection, but PCR and the mouse infectivity assay were most sensitive during late infection. Xenodiagnosis demonstrated that mice remain persistently infected through 55 days.</description><subject>Age Factors</subject><subject>Anaplasma phagocytophilum</subject><subject>Animals</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Disease Models, Animal</subject><subject>Disease Susceptibility</subject><subject>Disease Vectors</subject><subject>Ehrlichia - pathogenicity</subject><subject>Ehrlichiosis</subject><subject>Ehrlichiosis - etiology</subject><subject>Ehrlichiosis - immunology</subject><subject>Experimental bacterial diseases and models</subject><subject>Granulocytes</subject><subject>Granulocytes - microbiology</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Inoculation</subject><subject>Ixodes - microbiology</subject><subject>Leukocyte Count</subject><subject>Major Articles</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Mice, SCID</subject><subject>Spleen</subject><subject>Spleen - pathology</subject><subject>Ticks</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEURoMoWl__QBhE3I3m5p2lFJ_Ux6KguAmZmKGp044mM2D_vZGWCm5cZXEOHzcHoUPAZ4CVOOfACBUbaACcylIIoJtogDEhJSitd9BuSlOMMaNCbqNtzbRiXAwQXEc775vWLbrgistJbIKbhDaFVIR50U18MbJVG23XxkVx3_bJ76Ot2jbJH6zePTS-uhwPb8rR4_Xt8GJUOsZ5VwpVgdBV7UC9WSk1UCVc5bDTtfPYVxpqb2lNrKRegfWV80riGirBBQNP99DpcvYjtp-9T52ZheR809i5z2cYqSXhWIh_RRBEacZlFo__iNO2j_P8B0MI1YAlx79rLrYpRV-bjxhmNi4MYPMT2ixDZ_FotdZXM_-21lZlMz9ZcZucbeqc2YW01ghIlpv8zkxTTrzGFAMWmrDMyyUPqfNfa27juxGSSm5uXl4NvMLT-O7h2VzRb3KNm5w</recordid><startdate>19980301</startdate><enddate>19980301</enddate><creator>Hodzic, Emir</creator><creator>IJdo, Jacob W. 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| ispartof | The Journal of infectious diseases, 1998-03, Vol.177 (3), p.737-745 |
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| source | MEDLINE; Jstor Complete Legacy; Oxford Academic Journals (OUP) |
| subjects | Age Factors Anaplasma phagocytophilum Animals Bacterial diseases Biological and medical sciences Blood Disease Models, Animal Disease Susceptibility Disease Vectors Ehrlichia - pathogenicity Ehrlichiosis Ehrlichiosis - etiology Ehrlichiosis - immunology Experimental bacterial diseases and models Granulocytes Granulocytes - microbiology Humans Infections Infectious diseases Inoculation Ixodes - microbiology Leukocyte Count Major Articles Medical sciences Mice Mice, Inbred C3H Mice, SCID Spleen Spleen - pathology Ticks |
| title | Granulocytic Ehrlichiosis in the Laboratory Mouse |
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