Cholinergic Innervation of Fetal Neocortical Transplants Is Increased after Neutralization of Myelin-Associated Neurite Growth Inhibitors

Fetal neocortical transplants placed into adult neocortical sensorimotor aspiration lesions are known to receive afferent input from the adult host rat brain. As this input is less dense than normal, the present study was designed to investigate whether neutralization of myelin-associated neurite gr...

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Veröffentlicht in:Experimental neurology 1998-02, Vol.149 (2), p.390-397
Hauptverfasser: Schulz, M.K., Schnell, L., Castro, A.J., Schwab, M.E., Kartje, G.L.
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container_end_page 397
container_issue 2
container_start_page 390
container_title Experimental neurology
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creator Schulz, M.K.
Schnell, L.
Castro, A.J.
Schwab, M.E.
Kartje, G.L.
description Fetal neocortical transplants placed into adult neocortical sensorimotor aspiration lesions are known to receive afferent input from the adult host rat brain. As this input is less dense than normal, the present study was designed to investigate whether neutralization of myelin-associated neurite growth inhibitors NI-35/250 might promote host derived cholinergic innervation of fetal neocortical transplants. Adult rats received unilateral sensorimotor cortical aspiration lesions, and block grafts from embryonic day 14–15 neocortical tissue were placed immediately into the lesion cavities. Mouse hybridoma cells secreting either the monoclonal antibody IN-1, which blocks neurite growth inhibitors NI-35/250, or a control antibody or medium without cells were applied in millipore filter capsules directly over the fetal graft tissue. The brains were processed 12 weeks later for the visualization of acetylcholinesterase-positive, presumptive cholinergic fibers. We found an enhancement in the cholinergic innervation of fetal grafts in the recipients treated with the antibody IN-1 both in terms of fibers growing into the graft and of density within the center of the grafts. These results indicate that myelin-associated neurite growth inhibitors are involved in the development of host–transplant connectivity in the adult brain.
doi_str_mv 10.1006/exnr.1997.6731
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Psychology</topic><topic>Hybridomas - transplantation</topic><topic>Male</topic><topic>mature CNS</topic><topic>Mice</topic><topic>Myelin Sheath - immunology</topic><topic>Myelin Sheath - physiology</topic><topic>Neocortex - physiology</topic><topic>Neocortex - transplantation</topic><topic>Nerve Fibers - physiology</topic><topic>Nerve Regeneration</topic><topic>Neurites - immunology</topic><topic>Neurites - physiology</topic><topic>neuronal grafts</topic><topic>neuronal plasticity</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>Somatosensory Cortex - pathology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schulz, M.K.</creatorcontrib><creatorcontrib>Schnell, L.</creatorcontrib><creatorcontrib>Castro, A.J.</creatorcontrib><creatorcontrib>Schwab, M.E.</creatorcontrib><creatorcontrib>Kartje, G.L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schulz, M.K.</au><au>Schnell, L.</au><au>Castro, A.J.</au><au>Schwab, M.E.</au><au>Kartje, G.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cholinergic Innervation of Fetal Neocortical Transplants Is Increased after Neutralization of Myelin-Associated Neurite Growth Inhibitors</atitle><jtitle>Experimental neurology</jtitle><addtitle>Exp Neurol</addtitle><date>1998-02-01</date><risdate>1998</risdate><volume>149</volume><issue>2</issue><spage>390</spage><epage>397</epage><pages>390-397</pages><issn>0014-4886</issn><eissn>1090-2430</eissn><coden>EXNEAC</coden><abstract>Fetal neocortical transplants placed into adult neocortical sensorimotor aspiration lesions are known to receive afferent input from the adult host rat brain. 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subjects Acetylcholinesterase - analysis
Afferent Pathways - physiology
Animals
Antibodies, Monoclonal
axonal regeneration
Biological and medical sciences
Brain Tissue Transplantation - physiology
cholinergic system
Development. Senescence. Regeneration. Transplantation
Fetal Tissue Transplantation - physiology
Fundamental and applied biological sciences. Psychology
Hybridomas - transplantation
Male
mature CNS
Mice
Myelin Sheath - immunology
Myelin Sheath - physiology
Neocortex - physiology
Neocortex - transplantation
Nerve Fibers - physiology
Nerve Regeneration
Neurites - immunology
Neurites - physiology
neuronal grafts
neuronal plasticity
Rats
Rats, Inbred Lew
Somatosensory Cortex - pathology
Vertebrates: nervous system and sense organs
title Cholinergic Innervation of Fetal Neocortical Transplants Is Increased after Neutralization of Myelin-Associated Neurite Growth Inhibitors
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