Estradiol binding to cell surface raises cytosolic free calcium in T cells

The Fura-2 method is used to examine a possible action of 17β-estradiol (E 2) on [Ca 2+] i of splenic T cells isolated from female C57BL/10 mice. E 2 concentrations between 10 fM and 10 nM induce a rapid and dose-dependent increase in [Ca 2+] i due to Ca 2+ influx and release of Ca 2+ from intracell...

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Veröffentlicht in:	FEBS letters 1998-02, Vol.422 (3), p.349-353
Hauptverfasser:	Benten, W.Peter M, Lieberherr, Michèle, Giese, Günter, Wunderlich, Frank
Format:	Artikel
Sprache:	eng
Schlagworte:	[Ca2+]i, intracellular free Ca2+ concentration AIDS/HIV Animals BSA, bovine serum albumin Ca 2 Ca2 Calcium - metabolism CD4-Positive T-Lymphocytes - metabolism CD8-Positive T-Lymphocytes - metabolism Cell Membrane - metabolism ConA, concanavalin A Cytosol - metabolism E2, 17β-estradiol EGTA, ethylene glycol-bis(2-aminoethyl ether)-N,N,N′,N′,-tetraacetic acid Estradiol Estradiol - metabolism Estrogen Antagonists - pharmacology Estrogen receptor Female FITC, fluorescein isothiocyanate In Vitro Techniques Membrane receptor Mice Mice, Inbred C57BL PE, phycoerythrin Protein Binding Receptors, Estradiol - metabolism Spleen - cytology T cell T-Lymphocytes - drug effects T-Lymphocytes - metabolism Tamoxifen - pharmacology
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