Expression and Purification of Enzymatically Active Recombinant Granzyme B in a Baculovirus System

Granzyme B (GranB), a serine protease stored in the granules of cytotoxic T lymphocytes and natural killer cells, can initiate target cell apoptosis. To produce large amounts of purified active enzyme, recombinant murine granzyme B (rGranB) was expressed from baculovirus in insect cells. The express...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Biochemical and biophysical research communications 1998-02, Vol.243 (2), p.384-389
Hauptverfasser:	Xia, Zhinan, Kam, Chih-Min, Huang, Chifu, Powers, James C., Mandle, Robert J., Stevens, Richard L., Lieberman, Judy
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Baculoviridae - genetics Cysteine Endopeptidases - metabolism DNA Fragmentation - genetics Gene Expression - genetics Granzymes Killer Cells, Natural - enzymology Kinetics Peptides - metabolism Protein Precursors - metabolism Recombinant Proteins - genetics Serine Endopeptidases - genetics Serine Endopeptidases - isolation & purification Spodoptera - genetics T-Lymphocytes, Cytotoxic - enzymology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!