Isolation and characterization of the human hepatic lipase gene
Overlapping bacterial phage and cosmid genomic clones were isolated spanning an area of approximately 60 kilobases that contains the human hepatic lipase (HL) gene. It is composed of 9 exons spanning approximately 35 kilobases of DNA. The entire coding regions, the 5'-flanking sequences, and th...
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| Veröffentlicht in: | The Journal of biological chemistry 1990-04, Vol.265 (12), p.6552-6555 |
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| container_title | The Journal of biological chemistry |
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| creator | AMEIS, D STAHNKE, G KOBAYASHI, J MCLEAN, J LEE, G BUSCHER, M SCHOTZ, M. C WILL, H |
| description | Overlapping bacterial phage and cosmid genomic clones were isolated spanning an area of approximately 60 kilobases that contains
the human hepatic lipase (HL) gene. It is composed of 9 exons spanning approximately 35 kilobases of DNA. The entire coding
regions, the 5'-flanking sequences, and the exon-intron junctions were sequenced. The intron positions correspond to those
of human lipoprotein lipase and canine pancreatic lipase, supporting the concept that these genes constitute a dispersed gene
family of lipases and have evolved by duplication of a common ancestral gene. A region of the HL gene, which displays a significant
homology with various other lipolytic enzymes and contains the putative catalytic site serine residue of HL, was encoded by
exon 4. A major transcription start site of the human HL gene was located by primer extension analysis, 43 nucleotides upstream
of the translation initiation codon. Two possible promoter elements were located 25 and 63 nucleotides upstream of the transcription
initiation site: a "TATA" box-like sequence, TAATA, and a sequence found in the promoter region of many liver-specific genes,
AGGTTAATTATTAAT. In addition, sequences homologous to glucocorticoid and cAMP-responsive elements were identified in the 5'-nontranscribed
region. |
| doi_str_mv | 10.1016/s0021-9258(19)39182-3 |
| format | Article |
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the human hepatic lipase (HL) gene. It is composed of 9 exons spanning approximately 35 kilobases of DNA. The entire coding
regions, the 5'-flanking sequences, and the exon-intron junctions were sequenced. The intron positions correspond to those
of human lipoprotein lipase and canine pancreatic lipase, supporting the concept that these genes constitute a dispersed gene
family of lipases and have evolved by duplication of a common ancestral gene. A region of the HL gene, which displays a significant
homology with various other lipolytic enzymes and contains the putative catalytic site serine residue of HL, was encoded by
exon 4. A major transcription start site of the human HL gene was located by primer extension analysis, 43 nucleotides upstream
of the translation initiation codon. Two possible promoter elements were located 25 and 63 nucleotides upstream of the transcription
initiation site: a "TATA" box-like sequence, TAATA, and a sequence found in the promoter region of many liver-specific genes,
AGGTTAATTATTAAT. In addition, sequences homologous to glucocorticoid and cAMP-responsive elements were identified in the 5'-nontranscribed
region.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/s0021-9258(19)39182-3</identifier><identifier>PMID: 2324091</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; Codon - genetics ; DNA - genetics ; DNA - isolation & purification ; Exons ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes. Genome ; Genomic Library ; Humans ; Lipase - genetics ; liver ; Liver - enzymology ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Restriction Mapping ; Species Specificity ; Transcription, Genetic</subject><ispartof>The Journal of biological chemistry, 1990-04, Vol.265 (12), p.6552-6555</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c505t-7d47a241027af87029ca611268a701052045a9e43885653b989b17a8d8689fc43</citedby><cites>FETCH-LOGICAL-c505t-7d47a241027af87029ca611268a701052045a9e43885653b989b17a8d8689fc43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6854635$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2324091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>AMEIS, D</creatorcontrib><creatorcontrib>STAHNKE, G</creatorcontrib><creatorcontrib>KOBAYASHI, J</creatorcontrib><creatorcontrib>MCLEAN, J</creatorcontrib><creatorcontrib>LEE, G</creatorcontrib><creatorcontrib>BUSCHER, M</creatorcontrib><creatorcontrib>SCHOTZ, M. C</creatorcontrib><creatorcontrib>WILL, H</creatorcontrib><title>Isolation and characterization of the human hepatic lipase gene</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Overlapping bacterial phage and cosmid genomic clones were isolated spanning an area of approximately 60 kilobases that contains
the human hepatic lipase (HL) gene. It is composed of 9 exons spanning approximately 35 kilobases of DNA. The entire coding
regions, the 5'-flanking sequences, and the exon-intron junctions were sequenced. The intron positions correspond to those
of human lipoprotein lipase and canine pancreatic lipase, supporting the concept that these genes constitute a dispersed gene
family of lipases and have evolved by duplication of a common ancestral gene. A region of the HL gene, which displays a significant
homology with various other lipolytic enzymes and contains the putative catalytic site serine residue of HL, was encoded by
exon 4. A major transcription start site of the human HL gene was located by primer extension analysis, 43 nucleotides upstream
of the translation initiation codon. Two possible promoter elements were located 25 and 63 nucleotides upstream of the transcription
initiation site: a "TATA" box-like sequence, TAATA, and a sequence found in the promoter region of many liver-specific genes,
AGGTTAATTATTAAT. In addition, sequences homologous to glucocorticoid and cAMP-responsive elements were identified in the 5'-nontranscribed
region.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Codon - genetics</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>Exons</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes. Genome</subject><subject>Genomic Library</subject><subject>Humans</subject><subject>Lipase - genetics</subject><subject>liver</subject><subject>Liver - enzymology</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Restriction Mapping</subject><subject>Species Specificity</subject><subject>Transcription, Genetic</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMlKBDEQhoMoOi6PIDQooofWVNLZTiLiBoIHFbyFmkzajvQyJj2IPr3dzjBX61JQ9dWf8BFyCPQcKMiLRCmD3DChT8GccQOa5XyDTIBqnnMBb5tkskZ2yG5KH3SowsA22WacFdTAhFw-pK7GPnRthu0scxVGdL2P4Wc57Mqsr3xWLRpss8rPh6nL6jDH5LN33_p9slVinfzBqu-R19ubl-v7_PHp7uH66jF3goo-V7NCISuAMoWlVpQZhxKASY2KAhWMFgKNL7jWQgo-NdpMQaGeaalN6Qq-R06WufPYfS586m0TkvN1ja3vFskqo8AMQf-CIISSoMZEsQRd7FKKvrTzGBqM3xaoHQ3b51GfHfVZMPbPsOXD3eHqgcW08bP11UrpsD9e7TE5rMuIrQtpjUktCsnHfx4tsSq8V18hejsNnat8Y5kUFpiVQjD-C-IQjHU</recordid><startdate>19900425</startdate><enddate>19900425</enddate><creator>AMEIS, D</creator><creator>STAHNKE, G</creator><creator>KOBAYASHI, J</creator><creator>MCLEAN, J</creator><creator>LEE, G</creator><creator>BUSCHER, M</creator><creator>SCHOTZ, M. 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C ; WILL, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-7d47a241027af87029ca611268a701052045a9e43885653b989b17a8d8689fc43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Codon - genetics</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>Exons</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes. Genome</topic><topic>Genomic Library</topic><topic>Humans</topic><topic>Lipase - genetics</topic><topic>liver</topic><topic>Liver - enzymology</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Restriction Mapping</topic><topic>Species Specificity</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>AMEIS, D</creatorcontrib><creatorcontrib>STAHNKE, G</creatorcontrib><creatorcontrib>KOBAYASHI, J</creatorcontrib><creatorcontrib>MCLEAN, J</creatorcontrib><creatorcontrib>LEE, G</creatorcontrib><creatorcontrib>BUSCHER, M</creatorcontrib><creatorcontrib>SCHOTZ, M. C</creatorcontrib><creatorcontrib>WILL, H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Human Genome Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>AMEIS, D</au><au>STAHNKE, G</au><au>KOBAYASHI, J</au><au>MCLEAN, J</au><au>LEE, G</au><au>BUSCHER, M</au><au>SCHOTZ, M. C</au><au>WILL, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of the human hepatic lipase gene</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1990-04-25</date><risdate>1990</risdate><volume>265</volume><issue>12</issue><spage>6552</spage><epage>6555</epage><pages>6552-6555</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Overlapping bacterial phage and cosmid genomic clones were isolated spanning an area of approximately 60 kilobases that contains
the human hepatic lipase (HL) gene. It is composed of 9 exons spanning approximately 35 kilobases of DNA. The entire coding
regions, the 5'-flanking sequences, and the exon-intron junctions were sequenced. The intron positions correspond to those
of human lipoprotein lipase and canine pancreatic lipase, supporting the concept that these genes constitute a dispersed gene
family of lipases and have evolved by duplication of a common ancestral gene. A region of the HL gene, which displays a significant
homology with various other lipolytic enzymes and contains the putative catalytic site serine residue of HL, was encoded by
exon 4. A major transcription start site of the human HL gene was located by primer extension analysis, 43 nucleotides upstream
of the translation initiation codon. Two possible promoter elements were located 25 and 63 nucleotides upstream of the transcription
initiation site: a "TATA" box-like sequence, TAATA, and a sequence found in the promoter region of many liver-specific genes,
AGGTTAATTATTAAT. In addition, sequences homologous to glucocorticoid and cAMP-responsive elements were identified in the 5'-nontranscribed
region.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>2324091</pmid><doi>10.1016/s0021-9258(19)39182-3</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Cloning, Molecular Codon - genetics DNA - genetics DNA - isolation & purification Exons Fundamental and applied biological sciences. Psychology Genes Genes. Genome Genomic Library Humans Lipase - genetics liver Liver - enzymology Molecular and cellular biology Molecular genetics Molecular Sequence Data Restriction Mapping Species Specificity Transcription, Genetic |
| title | Isolation and characterization of the human hepatic lipase gene |
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