Postprandial plasma vitamin A metabolism in humans: A reassessment of the use of plasma retinyl esters as markers for intestinally derived chylomicrons and their remnants
We investigated postprandial vitamin A metabolism by measuring retinyl ester, triglyceride, and apolipoprotein (apo)B-48 in the plasma lipoproteins of human subjects before and after fat-feeding. Following a 14-hour fast, eight healthy subjects (two men, six women, 28 to 79 years) were given a fat-r...
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| Veröffentlicht in: | Metabolism, clinical and experimental clinical and experimental, 1990-04, Vol.39 (4), p.357-365 |
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| creator | Krasinski, Stephen D. Cohn, Jeffrey S. Russell, Robert M. Schaefer, Ernst J. |
| description | We investigated postprandial vitamin A metabolism by measuring retinyl ester, triglyceride, and apolipoprotein (apo)B-48 in the plasma lipoproteins of human subjects before and after fat-feeding. Following a 14-hour fast, eight healthy subjects (two men, six women, 28 to 79 years) were given a fat-rich meal (1 g fat/kg body weight) containing vitamin A (40 retinol equivalents per kilogram body weight). Blood was collected every 3 hours for 12 hours and lipoproteins were isolated by sequential ultracentrifugation. Mean plasma retinyl ester concentration peaked 6 hours after the fat-rich meal, whereas mean plasma triglyceride peaked at 3 hours. Data obtained from hourly samples in 3 subjects showed that changes in the postprandial plasma concentration of retinyl ester occurred 1 to 2 hours after changes in the plasma triglyceride concentration. In triglyceride-rich lipoproteins (TRL) of d |
| doi_str_mv | 10.1016/0026-0495(90)90249-C |
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Following a 14-hour fast, eight healthy subjects (two men, six women, 28 to 79 years) were given a fat-rich meal (1 g fat/kg body weight) containing vitamin A (40 retinol equivalents per kilogram body weight). Blood was collected every 3 hours for 12 hours and lipoproteins were isolated by sequential ultracentrifugation. Mean plasma retinyl ester concentration peaked 6 hours after the fat-rich meal, whereas mean plasma triglyceride peaked at 3 hours. Data obtained from hourly samples in 3 subjects showed that changes in the postprandial plasma concentration of retinyl ester occurred 1 to 2 hours after changes in the plasma triglyceride concentration. In triglyceride-rich lipoproteins (TRL) of d<1.006 g/mL, retinyl ester similarly peaked at 6 hours, whereas triglyceride as well as apoB-48 peaked at 3 hours. Although retinyl esters were found mainly in TRL in the initial postprandial period (84%, 3 hours; 83%, 6 hours), in fasting and postprandial plasma, particularly 9 or more hours after fat-feeding, a large percentage of plasma retinyl esters were in low-density lipoproteins (LDL) (44%, fasting; 9%, 3 hours; 9%, 6 hours; 19%, 9 hours; 32%, 12 hours). A small percentage of retinyl esters were also found in postprandial high-density lipoproteins (HDL) (2% to 7%). ApoB-48 was not detected in LDL of fasting or postprandial plasma. We conclude from these data that retinyl esters do not always serve as markers for intestinal apoB-48-containing TRL in fasting or postprandial plasma.</description><identifier>ISSN: 0026-0495</identifier><identifier>EISSN: 1532-8600</identifier><identifier>DOI: 10.1016/0026-0495(90)90249-C</identifier><identifier>PMID: 2325560</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Aged ; Apolipoproteins E - blood ; Biomarkers - blood ; BLOOD ; BLOOD PLASMA ; Cholesterol - blood ; Chylomicrons - blood ; Eating ; Female ; FOOD INTAKE ; Humans ; INGESTION DE ALIMENTOS ; INTESTIN ; Intestinal Absorption ; INTESTINES ; INTESTINOS ; Kinetics ; LIPIDE ; LIPIDOS ; LIPIDS ; LIPOPROTEINAS ; LIPOPROTEINE ; LIPOPROTEINS ; Lipoproteins - blood ; LOW DENSITY LIPOPROTEIN ; Male ; METABOLISM ; METABOLISME ; METABOLISMO ; Middle Aged ; PLASMA SANGUIN ; PLASMA SANGUINEO ; PRISE ALIMENTAIRE ; RETINOL ; SANG ; SANGRE ; Time Factors ; TRIGLICERIDOS ; TRIGLYCERIDE ; TRIGLYCERIDES ; Triglycerides - blood ; Vitamin A - analogs & derivatives ; Vitamin A - blood</subject><ispartof>Metabolism, clinical and experimental, 1990-04, Vol.39 (4), p.357-365</ispartof><rights>1990</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-95cf79cb0853de4be482cf0cfde922c26704b89b6fe4df73ccf8267abda723dc3</citedby><cites>FETCH-LOGICAL-c427t-95cf79cb0853de4be482cf0cfde922c26704b89b6fe4df73ccf8267abda723dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0026-0495(90)90249-C$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2325560$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Krasinski, Stephen D.</creatorcontrib><creatorcontrib>Cohn, Jeffrey S.</creatorcontrib><creatorcontrib>Russell, Robert M.</creatorcontrib><creatorcontrib>Schaefer, Ernst J.</creatorcontrib><title>Postprandial plasma vitamin A metabolism in humans: A reassessment of the use of plasma retinyl esters as markers for intestinally derived chylomicrons and their remnants</title><title>Metabolism, clinical and experimental</title><addtitle>Metabolism</addtitle><description>We investigated postprandial vitamin A metabolism by measuring retinyl ester, triglyceride, and apolipoprotein (apo)B-48 in the plasma lipoproteins of human subjects before and after fat-feeding. Following a 14-hour fast, eight healthy subjects (two men, six women, 28 to 79 years) were given a fat-rich meal (1 g fat/kg body weight) containing vitamin A (40 retinol equivalents per kilogram body weight). Blood was collected every 3 hours for 12 hours and lipoproteins were isolated by sequential ultracentrifugation. Mean plasma retinyl ester concentration peaked 6 hours after the fat-rich meal, whereas mean plasma triglyceride peaked at 3 hours. Data obtained from hourly samples in 3 subjects showed that changes in the postprandial plasma concentration of retinyl ester occurred 1 to 2 hours after changes in the plasma triglyceride concentration. In triglyceride-rich lipoproteins (TRL) of d<1.006 g/mL, retinyl ester similarly peaked at 6 hours, whereas triglyceride as well as apoB-48 peaked at 3 hours. Although retinyl esters were found mainly in TRL in the initial postprandial period (84%, 3 hours; 83%, 6 hours), in fasting and postprandial plasma, particularly 9 or more hours after fat-feeding, a large percentage of plasma retinyl esters were in low-density lipoproteins (LDL) (44%, fasting; 9%, 3 hours; 9%, 6 hours; 19%, 9 hours; 32%, 12 hours). A small percentage of retinyl esters were also found in postprandial high-density lipoproteins (HDL) (2% to 7%). ApoB-48 was not detected in LDL of fasting or postprandial plasma. We conclude from these data that retinyl esters do not always serve as markers for intestinal apoB-48-containing TRL in fasting or postprandial plasma.</description><subject>Adult</subject><subject>Aged</subject><subject>Apolipoproteins E - blood</subject><subject>Biomarkers - blood</subject><subject>BLOOD</subject><subject>BLOOD PLASMA</subject><subject>Cholesterol - blood</subject><subject>Chylomicrons - blood</subject><subject>Eating</subject><subject>Female</subject><subject>FOOD INTAKE</subject><subject>Humans</subject><subject>INGESTION DE ALIMENTOS</subject><subject>INTESTIN</subject><subject>Intestinal Absorption</subject><subject>INTESTINES</subject><subject>INTESTINOS</subject><subject>Kinetics</subject><subject>LIPIDE</subject><subject>LIPIDOS</subject><subject>LIPIDS</subject><subject>LIPOPROTEINAS</subject><subject>LIPOPROTEINE</subject><subject>LIPOPROTEINS</subject><subject>Lipoproteins - blood</subject><subject>LOW DENSITY LIPOPROTEIN</subject><subject>Male</subject><subject>METABOLISM</subject><subject>METABOLISME</subject><subject>METABOLISMO</subject><subject>Middle Aged</subject><subject>PLASMA SANGUIN</subject><subject>PLASMA SANGUINEO</subject><subject>PRISE ALIMENTAIRE</subject><subject>RETINOL</subject><subject>SANG</subject><subject>SANGRE</subject><subject>Time Factors</subject><subject>TRIGLICERIDOS</subject><subject>TRIGLYCERIDE</subject><subject>TRIGLYCERIDES</subject><subject>Triglycerides - blood</subject><subject>Vitamin A - analogs & derivatives</subject><subject>Vitamin A - blood</subject><issn>0026-0495</issn><issn>1532-8600</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UUuPFCEQJkazzq7-AaMJJ6OHVpp-4sFkM1kfySaa6J4JDYWDAj1S9CTzl_yV0s5kj54o6ntQfEXI85q9qVndv2WM9xVrRfdKsNeC8VZU2wdkU3cNr8aesYdkc095TC4RfzLGhmHsL8gFb3jX9WxD_nydMe-TisYpT_deYVD04LIKLtJrGiCrafYOAy333RJUxHeln0AhAmKAmOlsad4BXRDW8uyRILt49BQwQ0KqkAaVfq2lnVMxywVwUXl_pAaSO4Chenf0c3A6zbEIolldXSpOIaqY8Ql5ZJVHeHo-r8jdh5vv20_V7ZePn7fXt5Vu-ZAr0Wk7CD2xsWsMtBO0I9eWaWtAcK55P7B2GsXUW2iNHRqt7ViaajJq4I3RzRV5efLdp_n3UsaUwaEG71WEeUE5iKEeed0UYnsilokRE1i5T6788ihrJtcVyTV_ueYvBZP_ViS3Rfbi7L9MAcy96LyTgj874VbNUv1IDuXdN8GajnWigO9PIJQEDg6SRO0gajAugc7SzO7_r_8FxfKt1A</recordid><startdate>19900401</startdate><enddate>19900401</enddate><creator>Krasinski, Stephen D.</creator><creator>Cohn, Jeffrey S.</creator><creator>Russell, Robert M.</creator><creator>Schaefer, Ernst J.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900401</creationdate><title>Postprandial plasma vitamin A metabolism in humans: A reassessment of the use of plasma retinyl esters as markers for intestinally derived chylomicrons and their remnants</title><author>Krasinski, Stephen D. ; Cohn, Jeffrey S. ; Russell, Robert M. ; Schaefer, Ernst J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-95cf79cb0853de4be482cf0cfde922c26704b89b6fe4df73ccf8267abda723dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Apolipoproteins E - blood</topic><topic>Biomarkers - blood</topic><topic>BLOOD</topic><topic>BLOOD PLASMA</topic><topic>Cholesterol - blood</topic><topic>Chylomicrons - blood</topic><topic>Eating</topic><topic>Female</topic><topic>FOOD INTAKE</topic><topic>Humans</topic><topic>INGESTION DE ALIMENTOS</topic><topic>INTESTIN</topic><topic>Intestinal Absorption</topic><topic>INTESTINES</topic><topic>INTESTINOS</topic><topic>Kinetics</topic><topic>LIPIDE</topic><topic>LIPIDOS</topic><topic>LIPIDS</topic><topic>LIPOPROTEINAS</topic><topic>LIPOPROTEINE</topic><topic>LIPOPROTEINS</topic><topic>Lipoproteins - blood</topic><topic>LOW DENSITY LIPOPROTEIN</topic><topic>Male</topic><topic>METABOLISM</topic><topic>METABOLISME</topic><topic>METABOLISMO</topic><topic>Middle Aged</topic><topic>PLASMA SANGUIN</topic><topic>PLASMA SANGUINEO</topic><topic>PRISE ALIMENTAIRE</topic><topic>RETINOL</topic><topic>SANG</topic><topic>SANGRE</topic><topic>Time Factors</topic><topic>TRIGLICERIDOS</topic><topic>TRIGLYCERIDE</topic><topic>TRIGLYCERIDES</topic><topic>Triglycerides - blood</topic><topic>Vitamin A - analogs & derivatives</topic><topic>Vitamin A - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krasinski, Stephen D.</creatorcontrib><creatorcontrib>Cohn, Jeffrey S.</creatorcontrib><creatorcontrib>Russell, Robert M.</creatorcontrib><creatorcontrib>Schaefer, Ernst J.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Metabolism, clinical and experimental</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krasinski, Stephen D.</au><au>Cohn, Jeffrey S.</au><au>Russell, Robert M.</au><au>Schaefer, Ernst J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Postprandial plasma vitamin A metabolism in humans: A reassessment of the use of plasma retinyl esters as markers for intestinally derived chylomicrons and their remnants</atitle><jtitle>Metabolism, clinical and experimental</jtitle><addtitle>Metabolism</addtitle><date>1990-04-01</date><risdate>1990</risdate><volume>39</volume><issue>4</issue><spage>357</spage><epage>365</epage><pages>357-365</pages><issn>0026-0495</issn><eissn>1532-8600</eissn><abstract>We investigated postprandial vitamin A metabolism by measuring retinyl ester, triglyceride, and apolipoprotein (apo)B-48 in the plasma lipoproteins of human subjects before and after fat-feeding. Following a 14-hour fast, eight healthy subjects (two men, six women, 28 to 79 years) were given a fat-rich meal (1 g fat/kg body weight) containing vitamin A (40 retinol equivalents per kilogram body weight). Blood was collected every 3 hours for 12 hours and lipoproteins were isolated by sequential ultracentrifugation. Mean plasma retinyl ester concentration peaked 6 hours after the fat-rich meal, whereas mean plasma triglyceride peaked at 3 hours. Data obtained from hourly samples in 3 subjects showed that changes in the postprandial plasma concentration of retinyl ester occurred 1 to 2 hours after changes in the plasma triglyceride concentration. In triglyceride-rich lipoproteins (TRL) of d<1.006 g/mL, retinyl ester similarly peaked at 6 hours, whereas triglyceride as well as apoB-48 peaked at 3 hours. Although retinyl esters were found mainly in TRL in the initial postprandial period (84%, 3 hours; 83%, 6 hours), in fasting and postprandial plasma, particularly 9 or more hours after fat-feeding, a large percentage of plasma retinyl esters were in low-density lipoproteins (LDL) (44%, fasting; 9%, 3 hours; 9%, 6 hours; 19%, 9 hours; 32%, 12 hours). A small percentage of retinyl esters were also found in postprandial high-density lipoproteins (HDL) (2% to 7%). ApoB-48 was not detected in LDL of fasting or postprandial plasma. We conclude from these data that retinyl esters do not always serve as markers for intestinal apoB-48-containing TRL in fasting or postprandial plasma.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>2325560</pmid><doi>10.1016/0026-0495(90)90249-C</doi><tpages>9</tpages></addata></record> |
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| subjects | Adult Aged Apolipoproteins E - blood Biomarkers - blood BLOOD BLOOD PLASMA Cholesterol - blood Chylomicrons - blood Eating Female FOOD INTAKE Humans INGESTION DE ALIMENTOS INTESTIN Intestinal Absorption INTESTINES INTESTINOS Kinetics LIPIDE LIPIDOS LIPIDS LIPOPROTEINAS LIPOPROTEINE LIPOPROTEINS Lipoproteins - blood LOW DENSITY LIPOPROTEIN Male METABOLISM METABOLISME METABOLISMO Middle Aged PLASMA SANGUIN PLASMA SANGUINEO PRISE ALIMENTAIRE RETINOL SANG SANGRE Time Factors TRIGLICERIDOS TRIGLYCERIDE TRIGLYCERIDES Triglycerides - blood Vitamin A - analogs & derivatives Vitamin A - blood |
| title | Postprandial plasma vitamin A metabolism in humans: A reassessment of the use of plasma retinyl esters as markers for intestinally derived chylomicrons and their remnants |
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