Structural analysis of a new cryptic plasmid pAR67 isolated from Ruminococcus albus AR67

The complete nucleotide sequence of a new cryptic plasmid pAR67 isolated from a rumen bacterium Ruminococcus albus AR67 has been determined. The plasmid pAR67 was 3419 bp in size with a 45% GC content. Two open reading frames, ORF1 and ORF2, encoding potential polypeptides of 285 and 165 amino acids...

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Veröffentlicht in:Plasmid 1998, Vol.39 (1), p.84-88
Hauptverfasser: Ohara, H, Miyagi, T, Kaneichi, K, Karita, S, Kobayashi, Y, Kimura, T, Sakka, K, Ohmiya, K
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container_end_page 88
container_issue 1
container_start_page 84
container_title Plasmid
container_volume 39
creator Ohara, H
Miyagi, T
Kaneichi, K
Karita, S
Kobayashi, Y
Kimura, T
Sakka, K
Ohmiya, K
description The complete nucleotide sequence of a new cryptic plasmid pAR67 isolated from a rumen bacterium Ruminococcus albus AR67 has been determined. The plasmid pAR67 was 3419 bp in size with a 45% GC content. Two open reading frames, ORF1 and ORF2, encoding potential polypeptides of 285 and 165 amino acids, with limited sequence similarity to replication and mobilization proteins, respectively, were identified within the sequence. The region upstream of ORF1 had an AT-rich (80%) segment followed by four 19-bp direct repeats, which is similar to the structural organization characteristic of replication origins of some bacterial plasmids.
doi_str_mv 10.1006/plas.1997.1324
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subjects Amino Acid Sequence
Bacteria, Anaerobic - genetics
Base Sequence
DNA, Bacterial
Molecular Sequence Data
Open Reading Frames
Plasmids
title Structural analysis of a new cryptic plasmid pAR67 isolated from Ruminococcus albus AR67
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