Immunochemical studies of the lipopolysaccharide O‐specific polysaccharide of Hafnia alvei PCM 1199 related to H. alvei PCM 1205
On the basis of chemical analyses and NMR spectroscopic studies, it was found that the O‐specific polysaccharide (O‐PS) isolated from the Hafnia alvei PCM 1199 lipopolysaccharide (LPS) is a glycerol teichoic‐acid‐like polymer having a repeating unit of the following structure : 3)‐β‐ D‐Quip4NAc‐(1←1...
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| Veröffentlicht in: | European journal of biochemistry 1998-02, Vol.251 (3), p.980-985 |
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| container_title | European journal of biochemistry |
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| creator | Katzenellenbogen, Ewa Kocharova, Nina A. Zatonsky, Georgy V. Mieszala, Malgorzata Gamian, Andrzej Bogulska, Maria Shashkov, Aleksander S. Romanowska, Elzbieta Knirel, Yuriy A. |
| description | On the basis of chemical analyses and NMR spectroscopic studies, it was found that the O‐specific polysaccharide (O‐PS) isolated from the Hafnia alvei PCM 1199 lipopolysaccharide (LPS) is a glycerol teichoic‐acid‐like polymer having a repeating unit of the following structure :
3)‐β‐ D‐Quip4NAc‐(1←1)‐Gro3P‐(O←3)‐β‐
D‐Galp‐(1←3)‐α‐
D‐GlcpNAc‐(1←
2
6
↑∣
1
OAc
β‐
D‐GlcpNAc
6
∣
OAc
where Qui4NAc is 4‐acetamido‐4,6‐dideoxyglucose and O‐acetylation at both positions is non‐stoichiometric. The glycosidic linkage of the lateral β‐
D‐GlcpNAc residue is acid‐labile and cleaved from the OPS during mild acid hydrolysis or dephosphorylation with 48 % hydrofluoric acid. Comparative analysis revealed that the structure of the H. alvei PCM 1199 O‐PS is similar to that of H. alvei PCM 1205, which differs in the presence of an additional lateral α‐
D‐Glcp residue and the position of one of the O‐acetyl groups only. Accordingly, serological tests revealed a high degree of serological similarity between LPSs and O‐PSs of the two H. alvei strains. |
| doi_str_mv | 10.1046/j.1432-1327.1998.2510980.x |
| format | Article |
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3)‐β‐ D‐Quip4NAc‐(1←1)‐Gro3P‐(O←3)‐β‐
D‐Galp‐(1←3)‐α‐
D‐GlcpNAc‐(1←
2
6
↑∣
1
OAc
β‐
D‐GlcpNAc
6
∣
OAc
where Qui4NAc is 4‐acetamido‐4,6‐dideoxyglucose and O‐acetylation at both positions is non‐stoichiometric. The glycosidic linkage of the lateral β‐
D‐GlcpNAc residue is acid‐labile and cleaved from the OPS during mild acid hydrolysis or dephosphorylation with 48 % hydrofluoric acid. Comparative analysis revealed that the structure of the H. alvei PCM 1199 O‐PS is similar to that of H. alvei PCM 1205, which differs in the presence of an additional lateral α‐
D‐Glcp residue and the position of one of the O‐acetyl groups only. Accordingly, serological tests revealed a high degree of serological similarity between LPSs and O‐PSs of the two H. alvei strains.</description><identifier>ISSN: 0014-2956</identifier><identifier>EISSN: 1432-1033</identifier><identifier>DOI: 10.1046/j.1432-1327.1998.2510980.x</identifier><identifier>PMID: 9490075</identifier><language>eng</language><publisher>Berlin & Heidelberg: Springer‐Verlag</publisher><subject>Carbohydrate Conformation ; Carbohydrate Sequence ; Carbohydrates - analysis ; enterobacteria ; Enterobacteriaceae - chemistry ; Enterobacteriaceae - immunology ; Hafnia alvei ; lipopolysaccharide ; Methylation ; Molecular Sequence Data ; O Antigens - chemistry ; O Antigens - isolation & purification ; O‐antigen ; Species Specificity ; Teichoic Acids - chemistry ; teichoic acid‐like polysaccharide</subject><ispartof>European journal of biochemistry, 1998-02, Vol.251 (3), p.980-985</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4260-597f0921630a64df7d3ad48cf95f9ee46c5298c467a3956cb4e48fb19499c87a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1432-1327.1998.2510980.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1432-1327.1998.2510980.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9490075$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Katzenellenbogen, Ewa</creatorcontrib><creatorcontrib>Kocharova, Nina A.</creatorcontrib><creatorcontrib>Zatonsky, Georgy V.</creatorcontrib><creatorcontrib>Mieszala, Malgorzata</creatorcontrib><creatorcontrib>Gamian, Andrzej</creatorcontrib><creatorcontrib>Bogulska, Maria</creatorcontrib><creatorcontrib>Shashkov, Aleksander S.</creatorcontrib><creatorcontrib>Romanowska, Elzbieta</creatorcontrib><creatorcontrib>Knirel, Yuriy A.</creatorcontrib><title>Immunochemical studies of the lipopolysaccharide O‐specific polysaccharide of Hafnia alvei PCM 1199 related to H. alvei PCM 1205</title><title>European journal of biochemistry</title><addtitle>Eur J Biochem</addtitle><description>On the basis of chemical analyses and NMR spectroscopic studies, it was found that the O‐specific polysaccharide (O‐PS) isolated from the Hafnia alvei PCM 1199 lipopolysaccharide (LPS) is a glycerol teichoic‐acid‐like polymer having a repeating unit of the following structure :
3)‐β‐ D‐Quip4NAc‐(1←1)‐Gro3P‐(O←3)‐β‐
D‐Galp‐(1←3)‐α‐
D‐GlcpNAc‐(1←
2
6
↑∣
1
OAc
β‐
D‐GlcpNAc
6
∣
OAc
where Qui4NAc is 4‐acetamido‐4,6‐dideoxyglucose and O‐acetylation at both positions is non‐stoichiometric. The glycosidic linkage of the lateral β‐
D‐GlcpNAc residue is acid‐labile and cleaved from the OPS during mild acid hydrolysis or dephosphorylation with 48 % hydrofluoric acid. Comparative analysis revealed that the structure of the H. alvei PCM 1199 O‐PS is similar to that of H. alvei PCM 1205, which differs in the presence of an additional lateral α‐
D‐Glcp residue and the position of one of the O‐acetyl groups only. Accordingly, serological tests revealed a high degree of serological similarity between LPSs and O‐PSs of the two H. alvei strains.</description><subject>Carbohydrate Conformation</subject><subject>Carbohydrate Sequence</subject><subject>Carbohydrates - analysis</subject><subject>enterobacteria</subject><subject>Enterobacteriaceae - chemistry</subject><subject>Enterobacteriaceae - immunology</subject><subject>Hafnia alvei</subject><subject>lipopolysaccharide</subject><subject>Methylation</subject><subject>Molecular Sequence Data</subject><subject>O Antigens - chemistry</subject><subject>O Antigens - isolation & purification</subject><subject>O‐antigen</subject><subject>Species Specificity</subject><subject>Teichoic Acids - chemistry</subject><subject>teichoic acid‐like polysaccharide</subject><issn>0014-2956</issn><issn>1432-1033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkcFq3DAQhkVoSbabPEJA9NCbnZEly1Zv7ZJ0AwkJtDkLrTxitchr17Lb7C30CfKMfZJ4WVNCb0GHQfrmn9HMT8hHBikDIS82KRM8SxjPipQpVaZZzkCVkD4ekdkBAefvyAyAiSRTuTwhH2LcAIBUsjgmx0oogCKfkT_XdT1sG7vG2lsTaOyHymOkjaP9GmnwbdM2YReNtWvT-Qrp3d-n59ii9c5b-h8bVUvjtt5QE36hp_eLW8rGH9IOg-mxon1Dl-lrmEF-St47EyKeTXFOHq4ufyyWyc3dt-vFl5vEikxCkqvCgcqY5GCkqFxRcVOJ0jqVO4UopM0zVVohC8PHie1KoCjdio2jKluOj3Py6VC37ZqfA8Ze1z5aDMFssRmiLlTBeDmeOfl8SLRdE2OHTredr0230wz03gC90fst670Bem-AngzQj6P4fOoyrGqs_kmnjY98ceC_fcDdGyrrq8uv36cbfwGTS5W2</recordid><startdate>199802</startdate><enddate>199802</enddate><creator>Katzenellenbogen, Ewa</creator><creator>Kocharova, Nina A.</creator><creator>Zatonsky, Georgy V.</creator><creator>Mieszala, Malgorzata</creator><creator>Gamian, Andrzej</creator><creator>Bogulska, Maria</creator><creator>Shashkov, Aleksander S.</creator><creator>Romanowska, Elzbieta</creator><creator>Knirel, Yuriy A.</creator><general>Springer‐Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199802</creationdate><title>Immunochemical studies of the lipopolysaccharide O‐specific polysaccharide of Hafnia alvei PCM 1199 related to H. alvei PCM 1205</title><author>Katzenellenbogen, Ewa ; Kocharova, Nina A. ; Zatonsky, Georgy V. ; Mieszala, Malgorzata ; Gamian, Andrzej ; Bogulska, Maria ; Shashkov, Aleksander S. ; Romanowska, Elzbieta ; Knirel, Yuriy A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4260-597f0921630a64df7d3ad48cf95f9ee46c5298c467a3956cb4e48fb19499c87a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Carbohydrate Conformation</topic><topic>Carbohydrate Sequence</topic><topic>Carbohydrates - analysis</topic><topic>enterobacteria</topic><topic>Enterobacteriaceae - chemistry</topic><topic>Enterobacteriaceae - immunology</topic><topic>Hafnia alvei</topic><topic>lipopolysaccharide</topic><topic>Methylation</topic><topic>Molecular Sequence Data</topic><topic>O Antigens - chemistry</topic><topic>O Antigens - isolation & purification</topic><topic>O‐antigen</topic><topic>Species Specificity</topic><topic>Teichoic Acids - chemistry</topic><topic>teichoic acid‐like polysaccharide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Katzenellenbogen, Ewa</creatorcontrib><creatorcontrib>Kocharova, Nina A.</creatorcontrib><creatorcontrib>Zatonsky, Georgy V.</creatorcontrib><creatorcontrib>Mieszala, Malgorzata</creatorcontrib><creatorcontrib>Gamian, Andrzej</creatorcontrib><creatorcontrib>Bogulska, Maria</creatorcontrib><creatorcontrib>Shashkov, Aleksander S.</creatorcontrib><creatorcontrib>Romanowska, Elzbieta</creatorcontrib><creatorcontrib>Knirel, Yuriy A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Katzenellenbogen, Ewa</au><au>Kocharova, Nina A.</au><au>Zatonsky, Georgy V.</au><au>Mieszala, Malgorzata</au><au>Gamian, Andrzej</au><au>Bogulska, Maria</au><au>Shashkov, Aleksander S.</au><au>Romanowska, Elzbieta</au><au>Knirel, Yuriy A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunochemical studies of the lipopolysaccharide O‐specific polysaccharide of Hafnia alvei PCM 1199 related to H. alvei PCM 1205</atitle><jtitle>European journal of biochemistry</jtitle><addtitle>Eur J Biochem</addtitle><date>1998-02</date><risdate>1998</risdate><volume>251</volume><issue>3</issue><spage>980</spage><epage>985</epage><pages>980-985</pages><issn>0014-2956</issn><eissn>1432-1033</eissn><abstract>On the basis of chemical analyses and NMR spectroscopic studies, it was found that the O‐specific polysaccharide (O‐PS) isolated from the Hafnia alvei PCM 1199 lipopolysaccharide (LPS) is a glycerol teichoic‐acid‐like polymer having a repeating unit of the following structure :
3)‐β‐ D‐Quip4NAc‐(1←1)‐Gro3P‐(O←3)‐β‐
D‐Galp‐(1←3)‐α‐
D‐GlcpNAc‐(1←
2
6
↑∣
1
OAc
β‐
D‐GlcpNAc
6
∣
OAc
where Qui4NAc is 4‐acetamido‐4,6‐dideoxyglucose and O‐acetylation at both positions is non‐stoichiometric. The glycosidic linkage of the lateral β‐
D‐GlcpNAc residue is acid‐labile and cleaved from the OPS during mild acid hydrolysis or dephosphorylation with 48 % hydrofluoric acid. Comparative analysis revealed that the structure of the H. alvei PCM 1199 O‐PS is similar to that of H. alvei PCM 1205, which differs in the presence of an additional lateral α‐
D‐Glcp residue and the position of one of the O‐acetyl groups only. Accordingly, serological tests revealed a high degree of serological similarity between LPSs and O‐PSs of the two H. alvei strains.</abstract><cop>Berlin & Heidelberg</cop><pub>Springer‐Verlag</pub><pmid>9490075</pmid><doi>10.1046/j.1432-1327.1998.2510980.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-2956 |
| ispartof | European journal of biochemistry, 1998-02, Vol.251 (3), p.980-985 |
| issn | 0014-2956 1432-1033 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79713838 |
| source | MEDLINE; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Carbohydrate Conformation Carbohydrate Sequence Carbohydrates - analysis enterobacteria Enterobacteriaceae - chemistry Enterobacteriaceae - immunology Hafnia alvei lipopolysaccharide Methylation Molecular Sequence Data O Antigens - chemistry O Antigens - isolation & purification O‐antigen Species Specificity Teichoic Acids - chemistry teichoic acid‐like polysaccharide |
| title | Immunochemical studies of the lipopolysaccharide O‐specific polysaccharide of Hafnia alvei PCM 1199 related to H. alvei PCM 1205 |
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