Effects of urea and oxygen tension on K flux in sickle cells
K influx and efflux (both ouabain- and bumetanide-resistant) in haemoglobin S-containing red cells (sickle cells) were markedly stimulated by urea (> 0.25 M). Stimulation was rapid and reversible. Volume-sensitive KCl cotransport in both HbA or HbS red cells is thought to be O2-dependent but we s...
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| Veröffentlicht in: | Pflügers Archiv 1998-04, Vol.435 (5), p.740-742 |
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| creator | Culliford, S J Ellory, J C Gibson, J S Speake, P F |
| description | K influx and efflux (both ouabain- and bumetanide-resistant) in haemoglobin S-containing red cells (sickle cells) were markedly stimulated by urea (> 0.25 M). Stimulation was rapid and reversible. Volume-sensitive KCl cotransport in both HbA or HbS red cells is thought to be O2-dependent but we show here that urea-stimulated K fluxes in sickle cells were largely insensitive to O2 tension. Urea-stimulated K fluxes were not inhibited by lowering the external Ca concentration (with EGTA) but were abolished by Cl-substitution (with MeSO4 or NO3) or pretreatment of cells with the protein phosphatase inhibitor, calyculin A (0.1 muM). Results are consistent with a stimulatory action of urea on the KCl cotransporter, independent of oxygen tension, mediated via the phosphorylation cascade which regulates the transporter. The importance of this effect to the physiology and pathology of sickle cells is discussed. |
| doi_str_mv | 10.1007/s004240050576 |
| format | Article |
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Stimulation was rapid and reversible. Volume-sensitive KCl cotransport in both HbA or HbS red cells is thought to be O2-dependent but we show here that urea-stimulated K fluxes in sickle cells were largely insensitive to O2 tension. Urea-stimulated K fluxes were not inhibited by lowering the external Ca concentration (with EGTA) but were abolished by Cl-substitution (with MeSO4 or NO3) or pretreatment of cells with the protein phosphatase inhibitor, calyculin A (0.1 muM). Results are consistent with a stimulatory action of urea on the KCl cotransporter, independent of oxygen tension, mediated via the phosphorylation cascade which regulates the transporter. The importance of this effect to the physiology and pathology of sickle cells is discussed.</description><identifier>ISSN: 0031-6768</identifier><identifier>EISSN: 1432-2013</identifier><identifier>DOI: 10.1007/s004240050576</identifier><identifier>PMID: 9479028</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Anemia, Sickle Cell - blood ; Chlorides - metabolism ; Erythrocytes - drug effects ; Erythrocytes - metabolism ; Hemoglobin, Sickle ; Humans ; In Vitro Techniques ; Ion Transport - drug effects ; Oxygen - blood ; Potassium - blood ; Proteins ; Sickle cell anemia ; Urea - metabolism ; Urea - pharmacology</subject><ispartof>Pflügers Archiv, 1998-04, Vol.435 (5), p.740-742</ispartof><rights>Springer-Verlag Berlin Heidelberg 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c315t-1c7fbc4b1293da45afbb8d1ae2166e3e7b72277d166e56808feef32f75acf50a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9479028$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Culliford, S J</creatorcontrib><creatorcontrib>Ellory, J C</creatorcontrib><creatorcontrib>Gibson, J S</creatorcontrib><creatorcontrib>Speake, P F</creatorcontrib><title>Effects of urea and oxygen tension on K flux in sickle cells</title><title>Pflügers Archiv</title><addtitle>Pflugers Arch</addtitle><description>K influx and efflux (both ouabain- and bumetanide-resistant) in haemoglobin S-containing red cells (sickle cells) were markedly stimulated by urea (> 0.25 M). 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The importance of this effect to the physiology and pathology of sickle cells is discussed.</description><subject>Anemia, Sickle Cell - blood</subject><subject>Chlorides - metabolism</subject><subject>Erythrocytes - drug effects</subject><subject>Erythrocytes - metabolism</subject><subject>Hemoglobin, Sickle</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Ion Transport - drug effects</subject><subject>Oxygen - blood</subject><subject>Potassium - blood</subject><subject>Proteins</subject><subject>Sickle cell anemia</subject><subject>Urea - metabolism</subject><subject>Urea - pharmacology</subject><issn>0031-6768</issn><issn>1432-2013</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkE1Lw0AQhhdRaq0ePQqLB2_R2e8NeJFSP7DgRc9hs5mV1DSp2QTaf29Ci6AwMAw8vLzzEHLJ4JYBmLsIILkEUKCMPiJTJgVPODBxTKYAgiXaaHtKzmJcAQCXlk_IJJUmBW6n5H4RAvou0ibQvkVHXV3QZrv7xJp2WMeyqekwrzRU_ZaWNY2l_6qQeqyqeE5OgqsiXhz2jHw8Lt7nz8ny7ell_rBMvGCqS5g3IfcyZzwVhZPKhTy3BXPImdYo0OSGc2OK8VLagg2IQfBglPNBgRMzcrPP3bTNd4-xy9ZlHBu4Gps-ZiY1YLVWA3j9D1w1fVsP3TJr5Pi8hAFK9pBvmxhbDNmmLdeu3WUMslFp9kfpwF8dQvt8jcUvfXAofgAnVW8s</recordid><startdate>19980401</startdate><enddate>19980401</enddate><creator>Culliford, S J</creator><creator>Ellory, J C</creator><creator>Gibson, J S</creator><creator>Speake, P F</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>19980401</creationdate><title>Effects of urea and oxygen tension on K flux in sickle cells</title><author>Culliford, S J ; 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| subjects | Anemia, Sickle Cell - blood Chlorides - metabolism Erythrocytes - drug effects Erythrocytes - metabolism Hemoglobin, Sickle Humans In Vitro Techniques Ion Transport - drug effects Oxygen - blood Potassium - blood Proteins Sickle cell anemia Urea - metabolism Urea - pharmacology |
| title | Effects of urea and oxygen tension on K flux in sickle cells |
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