Enzymatic activities in extracts of small (20 ± 5 mg) samples of prostatic carcinoma
Previously, we reported that the activities of several enzymes extracted from prostatic cancers were closely related to the histologic grades (Gleason's grades) of the cancers. The survival of patients was more closely correlated with certain enzymatic activities than with Gleason's grades...
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| Veröffentlicht in: | Analytical biochemistry 1990, Vol.184 (1), p.128-134 |
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| container_title | Analytical biochemistry |
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| creator | Rackley, Raymond R. Lewis, Terrence J. Bradley, Edwin L. Levin, Howard S. Resnick, Martin I. Pretlow, Theresa P. Pretlow, Thomas G. |
| description | Previously, we reported that the activities of several enzymes extracted from prostatic cancers were closely related to the histologic grades (Gleason's grades) of the cancers. The survival of patients was more closely correlated with certain enzymatic activities than with Gleason's grades. Unfortunately, because our assays required relatively large amounts of tissue, the increasing proportion of patients who have needle biopsies as their only surgical procedure had to be excluded from our previous study. We now report a method that permits multiple enzyme assays to be carried out with much smaller amounts of tissue. This method will enable us to avoid the selection inherent in the exclusion of patients who have only needle biopsies and should permit the prospective study of most patients with prostatic carcinoma. |
| doi_str_mv | 10.1016/0003-2697(90)90024-4 |
| format | Article |
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The survival of patients was more closely correlated with certain enzymatic activities than with Gleason's grades. Unfortunately, because our assays required relatively large amounts of tissue, the increasing proportion of patients who have needle biopsies as their only surgical procedure had to be excluded from our previous study. We now report a method that permits multiple enzyme assays to be carried out with much smaller amounts of tissue. This method will enable us to avoid the selection inherent in the exclusion of patients who have only needle biopsies and should permit the prospective study of most patients with prostatic carcinoma.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(90)90024-4</identifier><identifier>PMID: 1690957</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Arginase - metabolism ; beta-N-Acetylhexosaminidases - metabolism ; Biological and medical sciences ; Biopsy, Needle ; Buffers ; carcinoma ; Enzyme Stability ; Glucosephosphate Dehydrogenase - metabolism ; Gynecology. Andrology. Obstetrics ; Hexosaminidases - metabolism ; Humans ; Male ; Male genital diseases ; Medical sciences ; Microchemistry - methods ; Prostatic Hyperplasia - enzymology ; Prostatic Hyperplasia - pathology ; Prostatic Neoplasms - enzymology ; Sonication ; Tissue and Organ Procurement ; Tumors</subject><ispartof>Analytical biochemistry, 1990, Vol.184 (1), p.128-134</ispartof><rights>1990</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-822c0e74e603e5d9df39fdeb7d619bcef53b5c876bb27edbf1931f86623f1f03</citedby><cites>FETCH-LOGICAL-c417t-822c0e74e603e5d9df39fdeb7d619bcef53b5c876bb27edbf1931f86623f1f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(90)90024-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6685407$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1690957$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rackley, Raymond R.</creatorcontrib><creatorcontrib>Lewis, Terrence J.</creatorcontrib><creatorcontrib>Bradley, Edwin L.</creatorcontrib><creatorcontrib>Levin, Howard S.</creatorcontrib><creatorcontrib>Resnick, Martin I.</creatorcontrib><creatorcontrib>Pretlow, Theresa P.</creatorcontrib><creatorcontrib>Pretlow, Thomas G.</creatorcontrib><title>Enzymatic activities in extracts of small (20 ± 5 mg) samples of prostatic carcinoma</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Previously, we reported that the activities of several enzymes extracted from prostatic cancers were closely related to the histologic grades (Gleason's grades) of the cancers. The survival of patients was more closely correlated with certain enzymatic activities than with Gleason's grades. Unfortunately, because our assays required relatively large amounts of tissue, the increasing proportion of patients who have needle biopsies as their only surgical procedure had to be excluded from our previous study. We now report a method that permits multiple enzyme assays to be carried out with much smaller amounts of tissue. This method will enable us to avoid the selection inherent in the exclusion of patients who have only needle biopsies and should permit the prospective study of most patients with prostatic carcinoma.</description><subject>Arginase - metabolism</subject><subject>beta-N-Acetylhexosaminidases - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biopsy, Needle</subject><subject>Buffers</subject><subject>carcinoma</subject><subject>Enzyme Stability</subject><subject>Glucosephosphate Dehydrogenase - metabolism</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Hexosaminidases - metabolism</subject><subject>Humans</subject><subject>Male</subject><subject>Male genital diseases</subject><subject>Medical sciences</subject><subject>Microchemistry - methods</subject><subject>Prostatic Hyperplasia - enzymology</subject><subject>Prostatic Hyperplasia - pathology</subject><subject>Prostatic Neoplasms - enzymology</subject><subject>Sonication</subject><subject>Tissue and Organ Procurement</subject><subject>Tumors</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKJDEUhoPMoD098wYjZDGILkpPKqmkshGkcXRAcKPrkEqdSIa6tEl1Y_tWvoJPZvWFdjezCuT_zs85HyE_GZwzYPICAHiWS61ONZxpgFxk4oBMGGiZAQf9hUz2yBH5ltJfAMZEIQ_JIZMadKEm5PG6e121dgiOWjeEZRgCJho6ii9DHH8S7T1NrW0aepoDfX-jBW2fzmiy7bzBTTqPfRo2Dc5GF7q-td_JV2-bhD9275Q8_L5-mN1md_c3f2ZXd5kTTA1ZmecOUAmUwLGode259jVWqpZMVw59wavClUpWVa6wrjzTnPlSypx75oFPycm2dlzheYFpMG1IDpvGdtgvklFaQQmC_xdkhRCC5XoExRZ041EpojfzGFobV4aBWVs3a6VmrdRoMBvrRoxjx7v-RdVi_Tm01Tzmv3a5Tc42PtrOhbTHpCwLAWvscovh6GwZMJrkAnYO6xDRDabuw7_3-ACP1J2F</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Rackley, Raymond R.</creator><creator>Lewis, Terrence J.</creator><creator>Bradley, Edwin L.</creator><creator>Levin, Howard S.</creator><creator>Resnick, Martin I.</creator><creator>Pretlow, Theresa P.</creator><creator>Pretlow, Thomas G.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>Enzymatic activities in extracts of small (20 ± 5 mg) samples of prostatic carcinoma</title><author>Rackley, Raymond R. ; Lewis, Terrence J. ; Bradley, Edwin L. ; Levin, Howard S. ; Resnick, Martin I. ; Pretlow, Theresa P. ; Pretlow, Thomas G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-822c0e74e603e5d9df39fdeb7d619bcef53b5c876bb27edbf1931f86623f1f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Arginase - metabolism</topic><topic>beta-N-Acetylhexosaminidases - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biopsy, Needle</topic><topic>Buffers</topic><topic>carcinoma</topic><topic>Enzyme Stability</topic><topic>Glucosephosphate Dehydrogenase - metabolism</topic><topic>Gynecology. 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Obstetrics</topic><topic>Hexosaminidases - metabolism</topic><topic>Humans</topic><topic>Male</topic><topic>Male genital diseases</topic><topic>Medical sciences</topic><topic>Microchemistry - methods</topic><topic>Prostatic Hyperplasia - enzymology</topic><topic>Prostatic Hyperplasia - pathology</topic><topic>Prostatic Neoplasms - enzymology</topic><topic>Sonication</topic><topic>Tissue and Organ Procurement</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rackley, Raymond R.</creatorcontrib><creatorcontrib>Lewis, Terrence J.</creatorcontrib><creatorcontrib>Bradley, Edwin L.</creatorcontrib><creatorcontrib>Levin, Howard S.</creatorcontrib><creatorcontrib>Resnick, Martin I.</creatorcontrib><creatorcontrib>Pretlow, Theresa P.</creatorcontrib><creatorcontrib>Pretlow, Thomas G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rackley, Raymond R.</au><au>Lewis, Terrence J.</au><au>Bradley, Edwin L.</au><au>Levin, Howard S.</au><au>Resnick, Martin I.</au><au>Pretlow, Theresa P.</au><au>Pretlow, Thomas G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic activities in extracts of small (20 ± 5 mg) samples of prostatic carcinoma</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1990</date><risdate>1990</risdate><volume>184</volume><issue>1</issue><spage>128</spage><epage>134</epage><pages>128-134</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>Previously, we reported that the activities of several enzymes extracted from prostatic cancers were closely related to the histologic grades (Gleason's grades) of the cancers. 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| ispartof | Analytical biochemistry, 1990, Vol.184 (1), p.128-134 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Arginase - metabolism beta-N-Acetylhexosaminidases - metabolism Biological and medical sciences Biopsy, Needle Buffers carcinoma Enzyme Stability Glucosephosphate Dehydrogenase - metabolism Gynecology. Andrology. Obstetrics Hexosaminidases - metabolism Humans Male Male genital diseases Medical sciences Microchemistry - methods Prostatic Hyperplasia - enzymology Prostatic Hyperplasia - pathology Prostatic Neoplasms - enzymology Sonication Tissue and Organ Procurement Tumors |
| title | Enzymatic activities in extracts of small (20 ± 5 mg) samples of prostatic carcinoma |
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