Increased ornithine decarboxylase activity and protein level in the cortex following traumatic brain injury in rats
There is increasing evidence that the elevated levels of polyamines play an important role in the secondary injury following traumatic brain injury (TBI). Ornithine decarboxylase (ODC) is the rate-limiting enzyme of polyamine biosynthesis. Presently, we measured the ODC protein levels by Western blo...
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| Veröffentlicht in: | Brain research 1998-02, Vol.783 (1), p.163-166 |
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| creator | Raghavendra Rao, Vemuganti L Başkaya, Mustafa K Muralikrishna Rao, A Doǧan, Aclan Dempsey, Robert J |
| description | There is increasing evidence that the elevated levels of polyamines play an important role in the secondary injury following traumatic brain injury (TBI). Ornithine decarboxylase (ODC) is the rate-limiting enzyme of polyamine biosynthesis. Presently, we measured the ODC protein levels by Western blot analysis in the cerebral cortex of rats sacrificed at 2 h, 6 h, 24 h, 72 h and 168 h after controlled cortical impact injury. TBI resulted in a significant increase in ODC protein levels (2.5 to 5.5 fold,
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| doi_str_mv | 10.1016/S0006-8993(97)01301-2 |
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P<0.05) and enzyme activity (13 to 21 fold,
p<0.01) between 2 and 6 h after the injury. ODC protein levels and enzyme activity returned to normal, control levels by 72 h after the injury. Increased ODC protein and enzyme activity could contribute to vasogenic edema and the pathogenesis of neuronal dysfunction after TBI by stimulating the formation of polyamines.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/S0006-8993(97)01301-2</identifier><identifier>PMID: 9479066</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Blotting, Western ; Brain Injuries - metabolism ; Cerebral Cortex - metabolism ; Injuries of the nervous system and the skull. Diseases due to physical agents ; Medical sciences ; Nerve Tissue Proteins - metabolism ; Ornithine decarboxylase ; Ornithine Decarboxylase - metabolism ; Polyamine ; Rats ; Rats, Sprague-Dawley ; Traumas. Diseases due to physical agents ; Traumatic brain injury ; Western blotting</subject><ispartof>Brain research, 1998-02, Vol.783 (1), p.163-166</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-4e908106f3f25ae9456de99d6b0500a180c13b266d53130d9ff1abf9d25505c33</citedby><cites>FETCH-LOGICAL-c420t-4e908106f3f25ae9456de99d6b0500a180c13b266d53130d9ff1abf9d25505c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-8993(97)01301-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2176599$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9479066$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Raghavendra Rao, Vemuganti L</creatorcontrib><creatorcontrib>Başkaya, Mustafa K</creatorcontrib><creatorcontrib>Muralikrishna Rao, A</creatorcontrib><creatorcontrib>Doǧan, Aclan</creatorcontrib><creatorcontrib>Dempsey, Robert J</creatorcontrib><title>Increased ornithine decarboxylase activity and protein level in the cortex following traumatic brain injury in rats</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>There is increasing evidence that the elevated levels of polyamines play an important role in the secondary injury following traumatic brain injury (TBI). Ornithine decarboxylase (ODC) is the rate-limiting enzyme of polyamine biosynthesis. Presently, we measured the ODC protein levels by Western blot analysis in the cerebral cortex of rats sacrificed at 2 h, 6 h, 24 h, 72 h and 168 h after controlled cortical impact injury. TBI resulted in a significant increase in ODC protein levels (2.5 to 5.5 fold,
P<0.05) and enzyme activity (13 to 21 fold,
p<0.01) between 2 and 6 h after the injury. ODC protein levels and enzyme activity returned to normal, control levels by 72 h after the injury. Increased ODC protein and enzyme activity could contribute to vasogenic edema and the pathogenesis of neuronal dysfunction after TBI by stimulating the formation of polyamines.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Brain Injuries - metabolism</subject><subject>Cerebral Cortex - metabolism</subject><subject>Injuries of the nervous system and the skull. Diseases due to physical agents</subject><subject>Medical sciences</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Ornithine decarboxylase</subject><subject>Ornithine Decarboxylase - metabolism</subject><subject>Polyamine</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Traumas. Diseases due to physical agents</subject><subject>Traumatic brain injury</subject><subject>Western blotting</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v3CAQhlHVKt1s-xMicaiq5uB2wDY2pyqK-hEpUg9tzwjD0BB5IQW8zf77stnVXiMOA3qfGWbmJeSCwUcGTHz6CQCiGaVsP8jhElgLrOEvyIqNA28E7-AlWZ2Q1-Q85_v6bFsJZ-RMdoMEIVYk3wSTUGe0NKbgy50PSC0anab4uJurQLUpfuvLjupg6UOKBX2gM25xpvVS7pCamAo-UhfnOf7z4Q8tSS8bXbyhU9IV8uF-Sbs9nnTJb8grp-eMb49xTX5__fLr-ntz--PbzfXVbWM6DqXpUMLIQLjW8V6j7HphUUorJugBNBvBsHbiQti-rdNb6RzTk5OW9z30pm3X5P2hbm3674K5qI3PBudZB4xLVoMcYH-eBZngoxg6VsH-AJoUc07o1EPyG512ioHau6KeXFH7lSs5qCdXFK95F8cPlmmD9pR1tKHq7466zkbPLulgfD5hnA2iryXX5PMBw7q1rceksvEYDFqf0BRlo3-mkf_yOqpW</recordid><startdate>19980202</startdate><enddate>19980202</enddate><creator>Raghavendra Rao, Vemuganti L</creator><creator>Başkaya, Mustafa K</creator><creator>Muralikrishna Rao, A</creator><creator>Doǧan, Aclan</creator><creator>Dempsey, Robert J</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19980202</creationdate><title>Increased ornithine decarboxylase activity and protein level in the cortex following traumatic brain injury in rats</title><author>Raghavendra Rao, Vemuganti L ; Başkaya, Mustafa K ; Muralikrishna Rao, A ; Doǧan, Aclan ; Dempsey, Robert J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-4e908106f3f25ae9456de99d6b0500a180c13b266d53130d9ff1abf9d25505c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Brain Injuries - metabolism</topic><topic>Cerebral Cortex - metabolism</topic><topic>Injuries of the nervous system and the skull. Diseases due to physical agents</topic><topic>Medical sciences</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Ornithine decarboxylase</topic><topic>Ornithine Decarboxylase - metabolism</topic><topic>Polyamine</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Traumas. Diseases due to physical agents</topic><topic>Traumatic brain injury</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raghavendra Rao, Vemuganti L</creatorcontrib><creatorcontrib>Başkaya, Mustafa K</creatorcontrib><creatorcontrib>Muralikrishna Rao, A</creatorcontrib><creatorcontrib>Doǧan, Aclan</creatorcontrib><creatorcontrib>Dempsey, Robert J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raghavendra Rao, Vemuganti L</au><au>Başkaya, Mustafa K</au><au>Muralikrishna Rao, A</au><au>Doǧan, Aclan</au><au>Dempsey, Robert J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased ornithine decarboxylase activity and protein level in the cortex following traumatic brain injury in rats</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1998-02-02</date><risdate>1998</risdate><volume>783</volume><issue>1</issue><spage>163</spage><epage>166</epage><pages>163-166</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>There is increasing evidence that the elevated levels of polyamines play an important role in the secondary injury following traumatic brain injury (TBI). Ornithine decarboxylase (ODC) is the rate-limiting enzyme of polyamine biosynthesis. Presently, we measured the ODC protein levels by Western blot analysis in the cerebral cortex of rats sacrificed at 2 h, 6 h, 24 h, 72 h and 168 h after controlled cortical impact injury. TBI resulted in a significant increase in ODC protein levels (2.5 to 5.5 fold,
P<0.05) and enzyme activity (13 to 21 fold,
p<0.01) between 2 and 6 h after the injury. ODC protein levels and enzyme activity returned to normal, control levels by 72 h after the injury. Increased ODC protein and enzyme activity could contribute to vasogenic edema and the pathogenesis of neuronal dysfunction after TBI by stimulating the formation of polyamines.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>9479066</pmid><doi>10.1016/S0006-8993(97)01301-2</doi><tpages>4</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Blotting, Western Brain Injuries - metabolism Cerebral Cortex - metabolism Injuries of the nervous system and the skull. Diseases due to physical agents Medical sciences Nerve Tissue Proteins - metabolism Ornithine decarboxylase Ornithine Decarboxylase - metabolism Polyamine Rats Rats, Sprague-Dawley Traumas. Diseases due to physical agents Traumatic brain injury Western blotting |
| title | Increased ornithine decarboxylase activity and protein level in the cortex following traumatic brain injury in rats |
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