Direct Gene Transfer into Mouse Muscle in Vivo
RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and β-galactosidase were separately injected into mouse skeletal muscle in vivo. Protein expression was readily detected in all cases, and no special delivery system was required for these effects. The...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1990-03, Vol.247 (4949), p.1465-1468 |
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| container_issue | 4949 |
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| container_title | Science (American Association for the Advancement of Science) |
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| creator | Wolff, Jon A. Malone, Robert W. Williams, Phillip Chong, Wang Acsadi, Gyula Jani, Agnes Felgner, Philip L. |
| description | RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and β-galactosidase were separately injected into mouse skeletal muscle in vivo. Protein expression was readily detected in all cases, and no special delivery system was required for these effects. The extent of expression from both the RNA and DNA constructs was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions. In situ cytochemical staining for β-galactosidase activity was localized to muscle cells following injection of the β-galactosidase DNA vector. After injection of the DNA luciferase expression vector, luciferase activity was present in the muscle for at least 2 months. |
| doi_str_mv | 10.1126/science.1690918 |
| format | Article |
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Protein expression was readily detected in all cases, and no special delivery system was required for these effects. The extent of expression from both the RNA and DNA constructs was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions. In situ cytochemical staining for β-galactosidase activity was localized to muscle cells following injection of the β-galactosidase DNA vector. After injection of the DNA luciferase expression vector, luciferase activity was present in the muscle for at least 2 months.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1690918</identifier><identifier>PMID: 1690918</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Animals ; Avian Sarcoma Viruses - genetics ; beta-Galactosidase - biosynthesis ; beta-Galactosidase - genetics ; Biological and medical sciences ; Biotechnology ; Chloramphenicol O-Acetyltransferase - biosynthesis ; Chloramphenicol O-Acetyltransferase - genetics ; Circles ; Coleoptera - genetics ; DNA ; DNA - genetics ; Escherichia coli - genetics ; Fibroblasts ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene therapy ; Genetic engineering ; Genetic technics ; Genetic Vectors ; Genetics ; Histocytochemistry ; Luciferases - biosynthesis ; Luciferases - genetics ; Luciferin ; Methods. Procedures. Technologies ; Mice ; Muscle cells ; Muscles ; Muscles - enzymology ; Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) ; Plasmids ; Polynucleotides ; RNA ; RNA - genetics ; skeletal muscle ; Soot ; Transfection ; Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><ispartof>Science (American Association for the Advancement of Science), 1990-03, Vol.247 (4949), p.1465-1468</ispartof><rights>Copyright 1990 American Association for the Advancement of Science</rights><rights>1991 INIST-CNRS</rights><rights>COPYRIGHT 1990 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1990 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Mar 23, 1990</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c616t-769b33c99255902bcfceb69fda8c5a4221043392bcca98a9de161be3153b0203</citedby><cites>FETCH-LOGICAL-c616t-769b33c99255902bcfceb69fda8c5a4221043392bcca98a9de161be3153b0203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2874228$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2874228$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19391483$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1690918$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wolff, Jon A.</creatorcontrib><creatorcontrib>Malone, Robert W.</creatorcontrib><creatorcontrib>Williams, Phillip</creatorcontrib><creatorcontrib>Chong, Wang</creatorcontrib><creatorcontrib>Acsadi, Gyula</creatorcontrib><creatorcontrib>Jani, Agnes</creatorcontrib><creatorcontrib>Felgner, Philip L.</creatorcontrib><title>Direct Gene Transfer into Mouse Muscle in Vivo</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and β-galactosidase were separately injected into mouse skeletal muscle in vivo. Protein expression was readily detected in all cases, and no special delivery system was required for these effects. The extent of expression from both the RNA and DNA constructs was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions. In situ cytochemical staining for β-galactosidase activity was localized to muscle cells following injection of the β-galactosidase DNA vector. After injection of the DNA luciferase expression vector, luciferase activity was present in the muscle for at least 2 months.</description><subject>Animals</subject><subject>Avian Sarcoma Viruses - genetics</subject><subject>beta-Galactosidase - biosynthesis</subject><subject>beta-Galactosidase - genetics</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chloramphenicol O-Acetyltransferase - biosynthesis</subject><subject>Chloramphenicol O-Acetyltransferase - genetics</subject><subject>Circles</subject><subject>Coleoptera - genetics</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>Escherichia coli - genetics</subject><subject>Fibroblasts</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene therapy</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Vectors</subject><subject>Genetics</subject><subject>Histocytochemistry</subject><subject>Luciferases - biosynthesis</subject><subject>Luciferases - genetics</subject><subject>Luciferin</subject><subject>Methods. Procedures. Technologies</subject><subject>Mice</subject><subject>Muscle cells</subject><subject>Muscles</subject><subject>Muscles - enzymology</subject><subject>Photinus-luciferin 4-monooxygenase (ATP-hydrolysing)</subject><subject>Plasmids</subject><subject>Polynucleotides</subject><subject>RNA</subject><subject>RNA - genetics</subject><subject>skeletal muscle</subject><subject>Soot</subject><subject>Transfection</subject><subject>Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqN0sFv0zAUBnALgUYZnLmAFCHBDqOdHceOfRwFyqSOHqh2tRz3pXKVxpudoO2_56FG20AVVDlE8veL9ex8hLxmdMJYLs-S89A6mDCpqWbqCRkxqsVY55Q_JSNKuRwrWorn5EVKG0ox0_yIHA18RCaffQTXZTNoIVtG26YaYubbLmSXoU-QXfbJNYAr2ZX_GV6SZ7VtErwa3sdk-fXLcvptPF_MLqbn87GTTHbjUuqKc6d1LoSmeeVqB5XU9coqJ2yR54wWnGsMnNXK6hUwySrgTPCK4ujH5MNu2-sYbnpIndn65KBpbAs4lSl1SYUsFMKTf8OCs7LQQvx3SyaEpIIVCN_9BTehjy2e1uSMi0LmXCI63aG1bcD4tg5dtG6NlxhtE1qoPS6fKy2kEgz1xz0anxVsvdvDT_7gKDq47da2T8lc_Ph-qFxcHSo_zQ6UajZ_LE_3SReaBtZgsBDTxWN9ttMuhpQi1OY6-q2Nd4ZR87vLZuiyGcqJX7wd_kRfbWH14O_z90Nuk7NNje11Pj0wzTXDjqB7s3Ob1IV4n-eqxCYq_guII_-d</recordid><startdate>19900323</startdate><enddate>19900323</enddate><creator>Wolff, Jon A.</creator><creator>Malone, Robert W.</creator><creator>Williams, Phillip</creator><creator>Chong, Wang</creator><creator>Acsadi, Gyula</creator><creator>Jani, Agnes</creator><creator>Felgner, Philip L.</creator><general>American Society for the Advancement of Science</general><general>American Association for the Advancement of Science</general><general>The American Association for the Advancement of Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8GL</scope><scope>IBG</scope><scope>IOV</scope><scope>ISN</scope><scope>0-V</scope><scope>3V.</scope><scope>7QF</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88B</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CJNVE</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9-</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0K</scope><scope>M0P</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEDU</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>R05</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>19900323</creationdate><title>Direct Gene Transfer into Mouse Muscle in Vivo</title><author>Wolff, Jon A. ; Malone, Robert W. ; Williams, Phillip ; Chong, Wang ; Acsadi, Gyula ; Jani, Agnes ; Felgner, Philip L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c616t-769b33c99255902bcfceb69fda8c5a4221043392bcca98a9de161be3153b0203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Avian Sarcoma Viruses - genetics</topic><topic>beta-Galactosidase - biosynthesis</topic><topic>beta-Galactosidase - genetics</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Chloramphenicol O-Acetyltransferase - biosynthesis</topic><topic>Chloramphenicol O-Acetyltransferase - genetics</topic><topic>Circles</topic><topic>Coleoptera - genetics</topic><topic>DNA</topic><topic>DNA - genetics</topic><topic>Escherichia coli - genetics</topic><topic>Fibroblasts</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene therapy</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic Vectors</topic><topic>Genetics</topic><topic>Histocytochemistry</topic><topic>Luciferases - biosynthesis</topic><topic>Luciferases - genetics</topic><topic>Luciferin</topic><topic>Methods. Procedures. Technologies</topic><topic>Mice</topic><topic>Muscle cells</topic><topic>Muscles</topic><topic>Muscles - enzymology</topic><topic>Photinus-luciferin 4-monooxygenase (ATP-hydrolysing)</topic><topic>Plasmids</topic><topic>Polynucleotides</topic><topic>RNA</topic><topic>RNA - genetics</topic><topic>skeletal muscle</topic><topic>Soot</topic><topic>Transfection</topic><topic>Vectors (cloning, transfer, expression). 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chloramphenicol acetyltransferase, luciferase, and β-galactosidase were separately injected into mouse skeletal muscle in vivo. Protein expression was readily detected in all cases, and no special delivery system was required for these effects. The extent of expression from both the RNA and DNA constructs was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions. In situ cytochemical staining for β-galactosidase activity was localized to muscle cells following injection of the β-galactosidase DNA vector. After injection of the DNA luciferase expression vector, luciferase activity was present in the muscle for at least 2 months.</abstract><cop>Washington, DC</cop><pub>American Society for the Advancement of Science</pub><pmid>1690918</pmid><doi>10.1126/science.1690918</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1990-03, Vol.247 (4949), p.1465-1468 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79705648 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; American Association for the Advancement of Science |
| subjects | Animals Avian Sarcoma Viruses - genetics beta-Galactosidase - biosynthesis beta-Galactosidase - genetics Biological and medical sciences Biotechnology Chloramphenicol O-Acetyltransferase - biosynthesis Chloramphenicol O-Acetyltransferase - genetics Circles Coleoptera - genetics DNA DNA - genetics Escherichia coli - genetics Fibroblasts Fundamental and applied biological sciences. Psychology Gene Expression Gene therapy Genetic engineering Genetic technics Genetic Vectors Genetics Histocytochemistry Luciferases - biosynthesis Luciferases - genetics Luciferin Methods. Procedures. Technologies Mice Muscle cells Muscles Muscles - enzymology Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) Plasmids Polynucleotides RNA RNA - genetics skeletal muscle Soot Transfection Vectors (cloning, transfer, expression). Insertion sequences and transposons |
| title | Direct Gene Transfer into Mouse Muscle in Vivo |
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