Delivery of antigen-encoding plasmid DNA into the cytosol of macrophages by attenuated suicide Listeria monocytogenes

Eukaryotic expression vectors can be delivered to macrophages using attenuated self-destructing Listeria monocytogenes. L. monocytogenes cells are preferentially lysed in the host cell macrophage cytosol by the production of a P acta -dependent Listeria -specific phage lysin. Efficient expression of...

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Veröffentlicht in:	Nature biotechnology 1998-02, Vol.16 (2), p.181-185
Hauptverfasser:	Dietrich, Guido, Bubert, Andreas, Gentschev, Ivaylo, Sokolovic, Zeljka, Simm, Andreas, Catic, Andre, Kaufmann, Stefan H.E., Hess, Jürgen, Szalay, Aladar A., Goebel, Werner
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture Animals Bacterial Vaccines Bacteriolysis Bacteriophages - enzymology Bacteriophages - genetics Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Cytomegalovirus - genetics Cytosol - metabolism Endopeptidases - genetics Endopeptidases - metabolism Genes, Reporter Genetic Vectors Life Sciences Listeria monocytogenes - genetics Listeria monocytogenes - virology Macrophages - metabolism Mice Mice, Inbred BALB C Plasmids - genetics Promoter Regions, Genetic research-article Tumor Cells, Cultured Vaccines, Attenuated Vaccines, DNA
Online-Zugang:	Volltext
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