Strategies for optimizing heterologous protein expression in Escherichia coli

The many advantages of Escherichia coli have ensured that it remains a valuable host for the efficient, cost-effective and high-level production of heterologous proteins. Here, we describe the current status of this prokaryotic expression system and focus on strategies designed to maximize the yield...

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Veröffentlicht in:	Trends in biotechnology (Regular ed.) 1998-02, Vol.16 (2), p.54-60
Hauptverfasser:	Hannig, Gerhard, Makrides, Savvas C
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Codon Cytoplasm - metabolism Escherichia coli Escherichia coli - genetics expression systems Fermentation Fundamental and applied biological sciences. Psychology Gene expression Genetic engineering Genetic technics heterologous proteins Methods. Procedures. Technologies Modification of gene expression level Molecular and cellular biology Molecular genetics Promoter Regions, Genetic promoters recombinant proteins Recombinant Proteins - biosynthesis
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