Dictyostelium discoideum nuclear plasmid Ddp6 is a new member of the Ddp2 plasmid family
Ddp6 is a high-copy number, circular plasmid found in the nucleus of the simple eukaryote Dictyostelium discoideum wild isolate NC47.2. The complete nucleotide sequence, 5257 bp, shows that Ddp6 has a structure similar to that of other members of the Ddp2 plasmid family: a single long 2.8-kb open re...
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| Veröffentlicht in: | Current genetics 1998-01, Vol.33 (1), p.77-82 |
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| creator | Shammat, I M Gonzales, C M Welker, D L |
| description | Ddp6 is a high-copy number, circular plasmid found in the nucleus of the simple eukaryote Dictyostelium discoideum wild isolate NC47.2. The complete nucleotide sequence, 5257 bp, shows that Ddp6 has a structure similar to that of other members of the Ddp2 plasmid family: a single long 2.8-kb open reading frame (rep gene) and an inverted repeat containing a pair of 654-bp elements. A single constitutively expressed 3.3-kb transcript of the rep gene was detected in RNA prepared from vegetative and developmental cells. Maintenance assays revealed that sequences within the inverted repeat and the intact Ddp6 ORF are essential for maintenance of the plasmid. Mutation of the inverted repeat, or of the rep gene, lowered the plasmid copy number but did not affect autonomous replication of shuttle-vector constructs. Comparisons of the predicted protein products of the rep genes of five members of the Ddp2 plasmid family identified a set of ten conserved features distributed throughout the peptides. |
| doi_str_mv | 10.1007/s002940050311 |
| format | Article |
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The complete nucleotide sequence, 5257 bp, shows that Ddp6 has a structure similar to that of other members of the Ddp2 plasmid family: a single long 2.8-kb open reading frame (rep gene) and an inverted repeat containing a pair of 654-bp elements. A single constitutively expressed 3.3-kb transcript of the rep gene was detected in RNA prepared from vegetative and developmental cells. Maintenance assays revealed that sequences within the inverted repeat and the intact Ddp6 ORF are essential for maintenance of the plasmid. Mutation of the inverted repeat, or of the rep gene, lowered the plasmid copy number but did not affect autonomous replication of shuttle-vector constructs. 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Comparisons of the predicted protein products of the rep genes of five members of the Ddp2 plasmid family identified a set of ten conserved features distributed throughout the peptides.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Dictyostelium - genetics</subject><subject>DNA Replication</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - isolation & purification</subject><subject>Genetic Vectors</subject><subject>Molecular Sequence Data</subject><subject>Plasmids - genetics</subject><subject>Plasmids - isolation & purification</subject><subject>Protozoan Proteins</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Sequence Analysis, DNA</subject><issn>0172-8083</issn><issn>1432-0983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1Lw0AQhhdRaq0ePQp78had2U2z2aO0fkHBi4K3sNmdxZWkidkE6b83paXgSU8zzDy8w_AwdolwgwDqNgIInQLMQSIesSmmUiSgc3nMpoBKJDnk8pSdxfgJgCLXasImOlViHE_Z-zLYftPEnqow1NyFaJvgaGzXg63IdLytTKyD40vXZjxEbviavnlNdUkdbzzvP2i7EwfQmzpUm3N24k0V6WJfZ-zt4f518ZSsXh6fF3erxMo59InyUmU6R0LAXHjUaSktOrRgLAGh1wRgnMk06oysd1iCdj5TNjVOCCVn7HqX23bN10CxL-rxB6oqs6ZmiIXSClJM53-CmM0hV_AfMBUS9fZ0sgNt18TYkS_aLtSm2xQIxdZN8cvNyF_tg4eyJneg9zLkD3N5iFo</recordid><startdate>19980101</startdate><enddate>19980101</enddate><creator>Shammat, I M</creator><creator>Gonzales, C M</creator><creator>Welker, D L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19980101</creationdate><title>Dictyostelium discoideum nuclear plasmid Ddp6 is a new member of the Ddp2 plasmid family</title><author>Shammat, I M ; Gonzales, C M ; Welker, D L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-7f376981e10182f194b3c1d1c0ace0e1f9e00ada69196ecfd1b09df67c4ad2273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Dictyostelium - genetics</topic><topic>DNA Replication</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - isolation & purification</topic><topic>Genetic Vectors</topic><topic>Molecular Sequence Data</topic><topic>Plasmids - genetics</topic><topic>Plasmids - isolation & purification</topic><topic>Protozoan Proteins</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shammat, I M</creatorcontrib><creatorcontrib>Gonzales, C M</creatorcontrib><creatorcontrib>Welker, D L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shammat, I M</au><au>Gonzales, C M</au><au>Welker, D L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dictyostelium discoideum nuclear plasmid Ddp6 is a new member of the Ddp2 plasmid family</atitle><jtitle>Current genetics</jtitle><addtitle>Curr Genet</addtitle><date>1998-01-01</date><risdate>1998</risdate><volume>33</volume><issue>1</issue><spage>77</spage><epage>82</epage><pages>77-82</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><abstract>Ddp6 is a high-copy number, circular plasmid found in the nucleus of the simple eukaryote Dictyostelium discoideum wild isolate NC47.2. 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| fulltext | fulltext |
| identifier | ISSN: 0172-8083 |
| ispartof | Current genetics, 1998-01, Vol.33 (1), p.77-82 |
| issn | 0172-8083 1432-0983 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Amino Acid Sequence Animals Dictyostelium - genetics DNA Replication Fungal Proteins - genetics Fungal Proteins - isolation & purification Genetic Vectors Molecular Sequence Data Plasmids - genetics Plasmids - isolation & purification Protozoan Proteins Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA |
| title | Dictyostelium discoideum nuclear plasmid Ddp6 is a new member of the Ddp2 plasmid family |
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