A newly identified iron binding protein in duodenal mucosa of rats. Purification and characterization of mobilferrin
An iron binding protein with an approximate molecular mass of 56,000 daltons was purified to homogeneity from homogenates of rat duodenal mucosa. The protein was biochemically and immunologically distinct from transferrin and ferritin and competitively bound cobalt, copper, zinc, and lead. Each mole...
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| Veröffentlicht in: | The Journal of biological chemistry 1990-03, Vol.265 (9), p.5273-5279 |
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| container_title | The Journal of biological chemistry |
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| creator | CONRAD, M. E UMBREIT, J. N MOORE, E. G PETERSON, R. D. A JONES, M. B |
| description | An iron binding protein with an approximate molecular mass of 56,000 daltons was purified to homogeneity from homogenates
of rat duodenal mucosa. The protein was biochemically and immunologically distinct from transferrin and ferritin and competitively
bound cobalt, copper, zinc, and lead. Each molecule bound one molecule of iron with a Kd of 9 X 10(-5). Dissociation of iron
and the protein was accelerated at acid pH. Using an immunogold method, the protein was identified in the apical cytoplasm
of proximal small intestinal cells and was not observed elsewhere in the intestinal mucosa and in other body organs. It was
named mobilferrin from its city of origin and to differentiate it from other previously identified iron binding proteins. |
| doi_str_mv | 10.1016/S0021-9258(19)34117-1 |
| format | Article |
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of rat duodenal mucosa. The protein was biochemically and immunologically distinct from transferrin and ferritin and competitively
bound cobalt, copper, zinc, and lead. Each molecule bound one molecule of iron with a Kd of 9 X 10(-5). Dissociation of iron
and the protein was accelerated at acid pH. Using an immunogold method, the protein was identified in the apical cytoplasm
of proximal small intestinal cells and was not observed elsewhere in the intestinal mucosa and in other body organs. It was
named mobilferrin from its city of origin and to differentiate it from other previously identified iron binding proteins.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(19)34117-1</identifier><identifier>PMID: 2318893</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Amino Acids - analysis ; Analytical, structural and metabolic biochemistry ; Animals ; Binding and carrier proteins ; Biological and medical sciences ; Carrier Proteins - analysis ; Carrier Proteins - isolation & purification ; Carrier Proteins - metabolism ; Chromatography, Gel ; Chromatography, Ion Exchange ; duodenum ; Duodenum - metabolism ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; Immunohistochemistry ; Intestinal Mucosa - cytology ; Intestinal Mucosa - metabolism ; Iron - metabolism ; Kinetics ; mobilferrin ; Molecular Weight ; mucosa ; Proteins ; Rats ; Rats, Inbred Strains</subject><ispartof>The Journal of biological chemistry, 1990-03, Vol.265 (9), p.5273-5279</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-e4dadffd355f7b415a5626c3f650039c357cd40110439b3d99c78a4b5f66d9043</citedby><cites>FETCH-LOGICAL-c438t-e4dadffd355f7b415a5626c3f650039c357cd40110439b3d99c78a4b5f66d9043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6950297$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2318893$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CONRAD, M. E</creatorcontrib><creatorcontrib>UMBREIT, J. N</creatorcontrib><creatorcontrib>MOORE, E. G</creatorcontrib><creatorcontrib>PETERSON, R. D. A</creatorcontrib><creatorcontrib>JONES, M. B</creatorcontrib><title>A newly identified iron binding protein in duodenal mucosa of rats. Purification and characterization of mobilferrin</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>An iron binding protein with an approximate molecular mass of 56,000 daltons was purified to homogeneity from homogenates
of rat duodenal mucosa. The protein was biochemically and immunologically distinct from transferrin and ferritin and competitively
bound cobalt, copper, zinc, and lead. Each molecule bound one molecule of iron with a Kd of 9 X 10(-5). Dissociation of iron
and the protein was accelerated at acid pH. Using an immunogold method, the protein was identified in the apical cytoplasm
of proximal small intestinal cells and was not observed elsewhere in the intestinal mucosa and in other body organs. It was
named mobilferrin from its city of origin and to differentiate it from other previously identified iron binding proteins.</description><subject>Amino Acids - analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Binding and carrier proteins</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - analysis</subject><subject>Carrier Proteins - isolation & purification</subject><subject>Carrier Proteins - metabolism</subject><subject>Chromatography, Gel</subject><subject>Chromatography, Ion Exchange</subject><subject>duodenum</subject><subject>Duodenum - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Iron - metabolism</subject><subject>Kinetics</subject><subject>mobilferrin</subject><subject>Molecular Weight</subject><subject>mucosa</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1LHTEQhkNpsUfbnyAEKWIv1mY2m2RzKaJtQWhBBe9CNh-elN1Ek13E_vrmeA7ntmEgMPO8M8m8CB0DOQcC_NstIS00smX9GcivtAMQDbxDKyA9bSiDh_dotUc-osNS_pB6OgkH6KCl0PeSrtB8gaN7GV9xsC7OwQdnccgp4iFEG-IjfsppdiHiGnZJFdIjnhaTisbJ46znco5_L7kqjZ5DFeposVnrrM3scvi7TVZ0SkMYvcs5xE_og9djcZ939xG6v766u_zR3Pz6_vPy4qYxHe3nxnVWW-8tZcyLoQOmGW-5oZ4zQqg0lAljOwJAOioHaqU0otfdwDznVtbkETrd9q2feF5cmdUUinHjqKNLS1FCcikEiP-CUAezlkEF2RY0OZWSnVdPOUw6vyogamOLerNFbXauQKo3W9RGd7wbsAyTs3vVzoda_7Kr62L06LOOJpQ9xiUjrdy882SLrcPj-iVkp4aQzNpNquVMScVaQek_AAqhlg</recordid><startdate>19900325</startdate><enddate>19900325</enddate><creator>CONRAD, M. E</creator><creator>UMBREIT, J. N</creator><creator>MOORE, E. G</creator><creator>PETERSON, R. D. A</creator><creator>JONES, M. B</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19900325</creationdate><title>A newly identified iron binding protein in duodenal mucosa of rats. Purification and characterization of mobilferrin</title><author>CONRAD, M. E ; UMBREIT, J. N ; MOORE, E. G ; PETERSON, R. D. A ; JONES, M. B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-e4dadffd355f7b415a5626c3f650039c357cd40110439b3d99c78a4b5f66d9043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acids - analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Binding and carrier proteins</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - analysis</topic><topic>Carrier Proteins - isolation & purification</topic><topic>Carrier Proteins - metabolism</topic><topic>Chromatography, Gel</topic><topic>Chromatography, Ion Exchange</topic><topic>duodenum</topic><topic>Duodenum - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>Intestinal Mucosa - cytology</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Iron - metabolism</topic><topic>Kinetics</topic><topic>mobilferrin</topic><topic>Molecular Weight</topic><topic>mucosa</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CONRAD, M. E</creatorcontrib><creatorcontrib>UMBREIT, J. N</creatorcontrib><creatorcontrib>MOORE, E. G</creatorcontrib><creatorcontrib>PETERSON, R. D. A</creatorcontrib><creatorcontrib>JONES, M. 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B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A newly identified iron binding protein in duodenal mucosa of rats. Purification and characterization of mobilferrin</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1990-03-25</date><risdate>1990</risdate><volume>265</volume><issue>9</issue><spage>5273</spage><epage>5279</epage><pages>5273-5279</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>An iron binding protein with an approximate molecular mass of 56,000 daltons was purified to homogeneity from homogenates
of rat duodenal mucosa. The protein was biochemically and immunologically distinct from transferrin and ferritin and competitively
bound cobalt, copper, zinc, and lead. Each molecule bound one molecule of iron with a Kd of 9 X 10(-5). Dissociation of iron
and the protein was accelerated at acid pH. Using an immunogold method, the protein was identified in the apical cytoplasm
of proximal small intestinal cells and was not observed elsewhere in the intestinal mucosa and in other body organs. It was
named mobilferrin from its city of origin and to differentiate it from other previously identified iron binding proteins.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>2318893</pmid><doi>10.1016/S0021-9258(19)34117-1</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acids - analysis Analytical, structural and metabolic biochemistry Animals Binding and carrier proteins Biological and medical sciences Carrier Proteins - analysis Carrier Proteins - isolation & purification Carrier Proteins - metabolism Chromatography, Gel Chromatography, Ion Exchange duodenum Duodenum - metabolism Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Immunohistochemistry Intestinal Mucosa - cytology Intestinal Mucosa - metabolism Iron - metabolism Kinetics mobilferrin Molecular Weight mucosa Proteins Rats Rats, Inbred Strains |
| title | A newly identified iron binding protein in duodenal mucosa of rats. Purification and characterization of mobilferrin |
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