Assessment of simple colorimetric procedures to determine smoking status of diabetic subjects
The performance of a simple colorimetric assay for urinary nicotine metabolites to assess smoking status in diabetic subjects (n = 251) was investigated. Several variations of the colorimetric assay and a qualitative extraction procedure were evaluated in comparison with a cotinine immunoassay as th...
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| Veröffentlicht in: | Clinical chemistry (Baltimore, Md.) Md.), 1998-02, Vol.44 (2), p.275-280 |
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| container_title | Clinical chemistry (Baltimore, Md.) |
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| creator | Smith, Robert F Mather, Hugh M Ellard, Gordon A |
| description | The performance of a simple colorimetric assay for urinary nicotine metabolites to assess smoking status in diabetic subjects (n = 251) was investigated. Several variations of the colorimetric assay and a qualitative extraction procedure were evaluated in comparison with a cotinine immunoassay as the "gold standard." Among these, the best overall performance was achieved with the qualitative test (sensitivity 95%; specificity 100%). The quantitative measurement of total nicotine metabolites performed less well (sensitivity 92%; specificity 97%) but could be improved by incorporating a blank extraction (sensitivity 98%; specificity 98%). Allowance for diuresis appeared to offer no advantage over the other methods. These results support previous findings regarding the use of these colorimetric procedures in nondiabetic subjects and, contrary to other recent observations, their performance was not impaired in diabetic patients. |
| doi_str_mv | 10.1093/clinchem/44.2.275 |
| format | Article |
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Several variations of the colorimetric assay and a qualitative extraction procedure were evaluated in comparison with a cotinine immunoassay as the "gold standard." Among these, the best overall performance was achieved with the qualitative test (sensitivity 95%; specificity 100%). The quantitative measurement of total nicotine metabolites performed less well (sensitivity 92%; specificity 97%) but could be improved by incorporating a blank extraction (sensitivity 98%; specificity 98%). Allowance for diuresis appeared to offer no advantage over the other methods. These results support previous findings regarding the use of these colorimetric procedures in nondiabetic subjects and, contrary to other recent observations, their performance was not impaired in diabetic patients.</description><identifier>ISSN: 0009-9147</identifier><identifier>EISSN: 1530-8561</identifier><identifier>DOI: 10.1093/clinchem/44.2.275</identifier><identifier>PMID: 9474024</identifier><identifier>CODEN: CLCHAU</identifier><language>eng</language><publisher>Washington, DC: Am Assoc Clin Chem</publisher><subject>Biological and medical sciences ; Colorimetry - methods ; Creatinine - urine ; Diabetes Mellitus - urine ; Humans ; Medical sciences ; Nicotine - urine ; Regression Analysis ; Sensitivity and Specificity ; Smoking - urine ; Thiobarbiturates - urine ; Tobacco, tobacco smoking ; Toxicology</subject><ispartof>Clinical chemistry (Baltimore, Md.), 1998-02, Vol.44 (2), p.275-280</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-ad47bd6404a3b65aa545b6d73c8afe08b66ec4aaf115dbe5b9172697eaf41f063</citedby><cites>FETCH-LOGICAL-c398t-ad47bd6404a3b65aa545b6d73c8afe08b66ec4aaf115dbe5b9172697eaf41f063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,777,781,786,787,23911,23912,25121,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2165803$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9474024$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smith, Robert F</creatorcontrib><creatorcontrib>Mather, Hugh M</creatorcontrib><creatorcontrib>Ellard, Gordon A</creatorcontrib><title>Assessment of simple colorimetric procedures to determine smoking status of diabetic subjects</title><title>Clinical chemistry (Baltimore, Md.)</title><addtitle>Clin Chem</addtitle><description>The performance of a simple colorimetric assay for urinary nicotine metabolites to assess smoking status in diabetic subjects (n = 251) was investigated. 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These results support previous findings regarding the use of these colorimetric procedures in nondiabetic subjects and, contrary to other recent observations, their performance was not impaired in diabetic patients.</description><subject>Biological and medical sciences</subject><subject>Colorimetry - methods</subject><subject>Creatinine - urine</subject><subject>Diabetes Mellitus - urine</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Nicotine - urine</subject><subject>Regression Analysis</subject><subject>Sensitivity and Specificity</subject><subject>Smoking - urine</subject><subject>Thiobarbiturates - urine</subject><subject>Tobacco, tobacco smoking</subject><subject>Toxicology</subject><issn>0009-9147</issn><issn>1530-8561</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE9v1DAQxS0EKkvhA3BAygG4ZWsn_hMfq4oWpEpcyhFZtjPputjJ4nG06rfH1S57Go3m997MPEI-MrplVPdXPobZ7yBdcb7ttp0Sr8iGiZ62g5DsNdlQSnWrGVdvyTvEp9pyNcgLcqG54rTjG_L7GhEQE8ylWaYGQ9pHaPwSlxwSlBx8s8-Lh3HNgE1ZmhEK5BRmaDAtf8L82GCxZcUX9Risg1IluLon8AXfkzeTjQgfTvWS_Lr99nDzvb3_effj5vq-9b0eSmtHrtwoOeW2d1JYK7hwclS9H-wEdHBSgufWToyJ0YFwmqlOagV24myisr8kX4--9da_K2AxKaCHGO0My4pGaal7qYYKsiPo84KYYTL7-qfNz4ZR8xKp-R-p4dx0pkZaNZ9O5qtLMJ4Vpwzr_PNpbtHbOGU7-4BnrGNSDLSv2JcjtguPu0PIYDDZGKspM4fD4bzuHyI8kHs</recordid><startdate>19980201</startdate><enddate>19980201</enddate><creator>Smith, Robert F</creator><creator>Mather, Hugh M</creator><creator>Ellard, Gordon A</creator><general>Am Assoc Clin Chem</general><general>American Association for Clinical Chemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980201</creationdate><title>Assessment of simple colorimetric procedures to determine smoking status of diabetic subjects</title><author>Smith, Robert F ; Mather, Hugh M ; Ellard, Gordon A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-ad47bd6404a3b65aa545b6d73c8afe08b66ec4aaf115dbe5b9172697eaf41f063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biological and medical sciences</topic><topic>Colorimetry - methods</topic><topic>Creatinine - urine</topic><topic>Diabetes Mellitus - urine</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Nicotine - urine</topic><topic>Regression Analysis</topic><topic>Sensitivity and Specificity</topic><topic>Smoking - urine</topic><topic>Thiobarbiturates - urine</topic><topic>Tobacco, tobacco smoking</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith, Robert F</creatorcontrib><creatorcontrib>Mather, Hugh M</creatorcontrib><creatorcontrib>Ellard, Gordon A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith, Robert F</au><au>Mather, Hugh M</au><au>Ellard, Gordon A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of simple colorimetric procedures to determine smoking status of diabetic subjects</atitle><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle><addtitle>Clin Chem</addtitle><date>1998-02-01</date><risdate>1998</risdate><volume>44</volume><issue>2</issue><spage>275</spage><epage>280</epage><pages>275-280</pages><issn>0009-9147</issn><eissn>1530-8561</eissn><coden>CLCHAU</coden><abstract>The performance of a simple colorimetric assay for urinary nicotine metabolites to assess smoking status in diabetic subjects (n = 251) was investigated. Several variations of the colorimetric assay and a qualitative extraction procedure were evaluated in comparison with a cotinine immunoassay as the "gold standard." Among these, the best overall performance was achieved with the qualitative test (sensitivity 95%; specificity 100%). The quantitative measurement of total nicotine metabolites performed less well (sensitivity 92%; specificity 97%) but could be improved by incorporating a blank extraction (sensitivity 98%; specificity 98%). Allowance for diuresis appeared to offer no advantage over the other methods. These results support previous findings regarding the use of these colorimetric procedures in nondiabetic subjects and, contrary to other recent observations, their performance was not impaired in diabetic patients.</abstract><cop>Washington, DC</cop><pub>Am Assoc Clin Chem</pub><pmid>9474024</pmid><doi>10.1093/clinchem/44.2.275</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical chemistry (Baltimore, Md.), 1998-02, Vol.44 (2), p.275-280 |
| issn | 0009-9147 1530-8561 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79693678 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Biological and medical sciences Colorimetry - methods Creatinine - urine Diabetes Mellitus - urine Humans Medical sciences Nicotine - urine Regression Analysis Sensitivity and Specificity Smoking - urine Thiobarbiturates - urine Tobacco, tobacco smoking Toxicology |
| title | Assessment of simple colorimetric procedures to determine smoking status of diabetic subjects |
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