Increased thymocyte differentiation in myasthenia gravis: A dual-color immunofluorescence phenotypic analysis
Thymocytes express multiple, different surface antigens according to their stage of maturation. Surface differentiation antigens have been studied with the technique of simultaneous dual‐color, direct immunofluorescence in the thymuses of 20 patients with myasthenia gravis (MG) and 10 control subjec...
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| Veröffentlicht in: | Annals of neurology 1990-02, Vol.27 (2), p.174-180 |
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| container_title | Annals of neurology |
| container_volume | 27 |
| creator | Durelli, L. Massazza, U. Poccardi, G. Ferrio, M. F. Cavallo, R. Maggi, G. Casadio, C. Di Summa, M. Bergamini, L. |
| description | Thymocytes express multiple, different surface antigens according to their stage of maturation. Surface differentiation antigens have been studied with the technique of simultaneous dual‐color, direct immunofluorescence in the thymuses of 20 patients with myasthenia gravis (MG) and 10 control subjects with cardiac diseases. Fluorescein isothiocyanate‐conjugated and phycoerythrin‐conjugated monoclonal antibodies were used to stain thymic cell suspensions. A significant decrease in the percentage of immature and common thymocyte phenotypes (CD1+,3+ and CD4+,8+) and significant increase in the percentage of mature thymocyte phenotypes (CD1‐,3+; CD4+,8‐; and CD4‐,8+) and of B cells (CD20+) were found in MG thymuses compared with controls. These data, indicating an increased availability of mature, fully immunocompetent T and B cells, indirectly suggest the occurrence of an active immune response in MG thymus. |
| doi_str_mv | 10.1002/ana.410270213 |
| format | Article |
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A significant decrease in the percentage of immature and common thymocyte phenotypes (CD1+,3+ and CD4+,8+) and significant increase in the percentage of mature thymocyte phenotypes (CD1‐,3+; CD4+,8‐; and CD4‐,8+) and of B cells (CD20+) were found in MG thymuses compared with controls. These data, indicating an increased availability of mature, fully immunocompetent T and B cells, indirectly suggest the occurrence of an active immune response in MG thymus.</description><identifier>ISSN: 0364-5134</identifier><identifier>EISSN: 1531-8249</identifier><identifier>DOI: 10.1002/ana.410270213</identifier><identifier>PMID: 2317013</identifier><identifier>CODEN: ANNED3</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adult ; Aged ; Antigens, Differentiation, T-Lymphocyte - immunology ; Biological and medical sciences ; Diseases of striated muscles. Neuromuscular diseases ; Female ; Humans ; Immunohistochemistry ; Lymphocyte Activation ; Male ; Medical sciences ; Middle Aged ; Myasthenia Gravis - immunology ; Myasthenia Gravis - pathology ; Neurology ; Phenotype ; Thymus Gland - immunology ; Thymus Gland - pathology</subject><ispartof>Annals of neurology, 1990-02, Vol.27 (2), p.174-180</ispartof><rights>Copyright © 1990 American Neurological Association</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4983-461000b7aa0e22ff2f804dc6f5d871ae895084cc0420295027fcf75cbff852163</citedby><cites>FETCH-LOGICAL-c4983-461000b7aa0e22ff2f804dc6f5d871ae895084cc0420295027fcf75cbff852163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fana.410270213$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fana.410270213$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6843843$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2317013$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Durelli, L.</creatorcontrib><creatorcontrib>Massazza, U.</creatorcontrib><creatorcontrib>Poccardi, G.</creatorcontrib><creatorcontrib>Ferrio, M. F.</creatorcontrib><creatorcontrib>Cavallo, R.</creatorcontrib><creatorcontrib>Maggi, G.</creatorcontrib><creatorcontrib>Casadio, C.</creatorcontrib><creatorcontrib>Di Summa, M.</creatorcontrib><creatorcontrib>Bergamini, L.</creatorcontrib><title>Increased thymocyte differentiation in myasthenia gravis: A dual-color immunofluorescence phenotypic analysis</title><title>Annals of neurology</title><addtitle>Ann Neurol</addtitle><description>Thymocytes express multiple, different surface antigens according to their stage of maturation. Surface differentiation antigens have been studied with the technique of simultaneous dual‐color, direct immunofluorescence in the thymuses of 20 patients with myasthenia gravis (MG) and 10 control subjects with cardiac diseases. Fluorescein isothiocyanate‐conjugated and phycoerythrin‐conjugated monoclonal antibodies were used to stain thymic cell suspensions. A significant decrease in the percentage of immature and common thymocyte phenotypes (CD1+,3+ and CD4+,8+) and significant increase in the percentage of mature thymocyte phenotypes (CD1‐,3+; CD4+,8‐; and CD4‐,8+) and of B cells (CD20+) were found in MG thymuses compared with controls. These data, indicating an increased availability of mature, fully immunocompetent T and B cells, indirectly suggest the occurrence of an active immune response in MG thymus.</description><subject>Adult</subject><subject>Aged</subject><subject>Antigens, Differentiation, T-Lymphocyte - immunology</subject><subject>Biological and medical sciences</subject><subject>Diseases of striated muscles. Neuromuscular diseases</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Lymphocyte Activation</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Myasthenia Gravis - immunology</subject><subject>Myasthenia Gravis - pathology</subject><subject>Neurology</subject><subject>Phenotype</subject><subject>Thymus Gland - immunology</subject><subject>Thymus Gland - pathology</subject><issn>0364-5134</issn><issn>1531-8249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi0EKtvCkSOSD4hbyvgjccJtqdhStSqXIioultexqcGxFzuB5t_jaqNVT5UsjaV5ZubRi9AbAqcEgH5QQZ1yAlQAJewZWpGakaqlvHuOVsAaXtWE8ZfoOOdfANA1BI7QEWVEAGErNFwEnYzKpsfj3TxEPY8G985ak0wYnRpdDNgFPMwqj3cmOIV_JvXX5Y94jftJ-UpHHxN2wzCFaP0Uk8naBG3wruBxnHdO4-Lo5-zyK_TCKp_N66WeoG-bzzdnX6qrr-cXZ-urSvOuZRUvkgBboRQYSq2ltgXe68bWfSuIMm1XQ8u1Bk6Blj8VVltR6621bU1Jw07Q-_3eXYp_JpNHObhi5b0KJk5Ziq7pGOe8gNUe1CnmnIyVu-QGlWZJQD7EK4u6PMRb-LfL4mk7mP5AL3mW_rulr7JW3iYVtMsHrGk5K69gYo_9c97MT9-U6-v1Y4FF2OXR3B8mVfotG8FELb9fn8vm9vLT5vbHjdyw_7qoo48</recordid><startdate>199002</startdate><enddate>199002</enddate><creator>Durelli, L.</creator><creator>Massazza, U.</creator><creator>Poccardi, G.</creator><creator>Ferrio, M. F.</creator><creator>Cavallo, R.</creator><creator>Maggi, G.</creator><creator>Casadio, C.</creator><creator>Di Summa, M.</creator><creator>Bergamini, L.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Willey-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199002</creationdate><title>Increased thymocyte differentiation in myasthenia gravis: A dual-color immunofluorescence phenotypic analysis</title><author>Durelli, L. ; Massazza, U. ; Poccardi, G. ; Ferrio, M. F. ; Cavallo, R. ; Maggi, G. ; Casadio, C. ; Di Summa, M. ; Bergamini, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4983-461000b7aa0e22ff2f804dc6f5d871ae895084cc0420295027fcf75cbff852163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Antigens, Differentiation, T-Lymphocyte - immunology</topic><topic>Biological and medical sciences</topic><topic>Diseases of striated muscles. Neuromuscular diseases</topic><topic>Female</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Lymphocyte Activation</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Myasthenia Gravis - immunology</topic><topic>Myasthenia Gravis - pathology</topic><topic>Neurology</topic><topic>Phenotype</topic><topic>Thymus Gland - immunology</topic><topic>Thymus Gland - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Durelli, L.</creatorcontrib><creatorcontrib>Massazza, U.</creatorcontrib><creatorcontrib>Poccardi, G.</creatorcontrib><creatorcontrib>Ferrio, M. F.</creatorcontrib><creatorcontrib>Cavallo, R.</creatorcontrib><creatorcontrib>Maggi, G.</creatorcontrib><creatorcontrib>Casadio, C.</creatorcontrib><creatorcontrib>Di Summa, M.</creatorcontrib><creatorcontrib>Bergamini, L.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Durelli, L.</au><au>Massazza, U.</au><au>Poccardi, G.</au><au>Ferrio, M. 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Fluorescein isothiocyanate‐conjugated and phycoerythrin‐conjugated monoclonal antibodies were used to stain thymic cell suspensions. A significant decrease in the percentage of immature and common thymocyte phenotypes (CD1+,3+ and CD4+,8+) and significant increase in the percentage of mature thymocyte phenotypes (CD1‐,3+; CD4+,8‐; and CD4‐,8+) and of B cells (CD20+) were found in MG thymuses compared with controls. These data, indicating an increased availability of mature, fully immunocompetent T and B cells, indirectly suggest the occurrence of an active immune response in MG thymus.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>2317013</pmid><doi>10.1002/ana.410270213</doi><tpages>7</tpages></addata></record> |
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| ispartof | Annals of neurology, 1990-02, Vol.27 (2), p.174-180 |
| issn | 0364-5134 1531-8249 |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | Adult Aged Antigens, Differentiation, T-Lymphocyte - immunology Biological and medical sciences Diseases of striated muscles. Neuromuscular diseases Female Humans Immunohistochemistry Lymphocyte Activation Male Medical sciences Middle Aged Myasthenia Gravis - immunology Myasthenia Gravis - pathology Neurology Phenotype Thymus Gland - immunology Thymus Gland - pathology |
| title | Increased thymocyte differentiation in myasthenia gravis: A dual-color immunofluorescence phenotypic analysis |
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