Immunochemical assays of serum proteins: a European external quality assessment survey and the effects of calibration procedures on interlaboratory agreement
An external quality assessment survey of immunochemical assays of 9 proteins (immunoglobulins G, A and M, complement components C3 and C4, alpha 1 -antitrypsin, orosomucoid, haptoglobin and transferrin) in 5 European countries (Austria, France, Hungary, Italy and UK) showed inter-country differences...
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| Veröffentlicht in: | Clinica chimica acta 1990-01, Vol.187 (1), p.21-35 |
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| container_title | Clinica chimica acta |
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| creator | Bullock, David G. Dumont, Gilles Vassault, Anne Aguzzi, Francesco Chambers, Robin E. Ward, A.Milford Whicher, John T. Bienvenu, Jacques |
| description | An external quality assessment survey of immunochemical assays of 9 proteins (immunoglobulins G, A and M, complement components C3 and C4, alpha
1 -antitrypsin, orosomucoid, haptoglobin and transferrin) in 5 European countries (Austria, France, Hungary, Italy and UK) showed inter-country differences in the mean values obtained. Reprocessing of the results using one of the two specimens distributed as a ‘calibrant’ effectively eliminated or reduced substantially these differences. Consideration of the methods used by participants confirmed previous indications from national surveys that the differences were due to lack of agreement among commercial calibrants. Such interlaboratory variations were also minimised by the ‘calibration’ in this survey. The role of European working calibration materials in ensuring interlaboratory agreement on an international basis is discussed. |
| doi_str_mv | 10.1016/0009-8981(90)90258-T |
| format | Article |
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1 -antitrypsin, orosomucoid, haptoglobin and transferrin) in 5 European countries (Austria, France, Hungary, Italy and UK) showed inter-country differences in the mean values obtained. Reprocessing of the results using one of the two specimens distributed as a ‘calibrant’ effectively eliminated or reduced substantially these differences. Consideration of the methods used by participants confirmed previous indications from national surveys that the differences were due to lack of agreement among commercial calibrants. Such interlaboratory variations were also minimised by the ‘calibration’ in this survey. The role of European working calibration materials in ensuring interlaboratory agreement on an international basis is discussed.</description><subject>Analysis of complex biological substances</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Blood and biological fluids</subject><subject>Blood Proteins - analysis</subject><subject>Calibration</subject><subject>Calibration - standards</subject><subject>Europe</subject><subject>External quality assessment</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoassay - standards</subject><subject>Immunoassay - statistics & numerical data</subject><subject>Immunochemical protein assay</subject><subject>Interlaboratory agreement</subject><subject>Observer Variation</subject><subject>Quality Control</subject><subject>Reference material</subject><subject>Reference Standards</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcGOFCEURYnRjO3oH2jCwhhdlEJRXYALEzMZdZJJ3PSeUNTDwVRBD6-Y2B_jv0pNd3rpisC99_ByHyGvOfvIGe8_McZ0o7Ti7zX7oFm7Vc3uCdlwJUUjOt0-JZuz5Tl5gfi7XjvW8wty0QouddtvyN-beS4xuTuYg7MTtYj2gDR5ipDLTPc5LRAifqaWXpec9mAjhT8L5Fjd98VOYTmsKUCcIS4US36A-hJHutwBBe_BLY_Aig9DtktIccU6GEuGqkQaYuVNdkhVTbmGf2WAlfaSPPN2Qnh1Oi_J7tv17upHc_vz-83V19vGCdUvjQc5cs-lZLrzctspL3zXSyfaUfZgGbfctX6QTIx66zvhBPNitG5QFmwrxSV5d8TWse4L4GLmgA6myUZIBY3UveaKqWrsjkaXE2IGb_Y5zDYfDGdmXYpZGzdr40Yz87gUs6uxNyd-GWYYz6HTFqr-9qRbrC35bKMLeLbVz7nqebV9OdqgVvEQIBt0AWItMuRashlT-P8c_wB2Ba3L</recordid><startdate>19900131</startdate><enddate>19900131</enddate><creator>Bullock, David G.</creator><creator>Dumont, Gilles</creator><creator>Vassault, Anne</creator><creator>Aguzzi, Francesco</creator><creator>Chambers, Robin E.</creator><creator>Ward, A.Milford</creator><creator>Whicher, John T.</creator><creator>Bienvenu, Jacques</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900131</creationdate><title>Immunochemical assays of serum proteins: a European external quality assessment survey and the effects of calibration procedures on interlaboratory agreement</title><author>Bullock, David G. ; Dumont, Gilles ; Vassault, Anne ; Aguzzi, Francesco ; Chambers, Robin E. ; Ward, A.Milford ; Whicher, John T. ; Bienvenu, Jacques</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-fe7d1f177094f7548f3f467c32d76ea01a1c2fb703d95f43c30f3dacb8aea273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Analysis of complex biological substances</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Blood and biological fluids</topic><topic>Blood Proteins - analysis</topic><topic>Calibration</topic><topic>Calibration - standards</topic><topic>Europe</topic><topic>External quality assessment</topic><topic>Fundamental and applied biological sciences. 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1 -antitrypsin, orosomucoid, haptoglobin and transferrin) in 5 European countries (Austria, France, Hungary, Italy and UK) showed inter-country differences in the mean values obtained. Reprocessing of the results using one of the two specimens distributed as a ‘calibrant’ effectively eliminated or reduced substantially these differences. Consideration of the methods used by participants confirmed previous indications from national surveys that the differences were due to lack of agreement among commercial calibrants. Such interlaboratory variations were also minimised by the ‘calibration’ in this survey. The role of European working calibration materials in ensuring interlaboratory agreement on an international basis is discussed.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>2317926</pmid><doi>10.1016/0009-8981(90)90258-T</doi><tpages>15</tpages></addata></record> |
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| ispartof | Clinica chimica acta, 1990-01, Vol.187 (1), p.21-35 |
| issn | 0009-8981 1873-3492 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Analysis of complex biological substances Analytical, structural and metabolic biochemistry Biological and medical sciences Blood and biological fluids Blood Proteins - analysis Calibration Calibration - standards Europe External quality assessment Fundamental and applied biological sciences. Psychology Humans Immunoassay - standards Immunoassay - statistics & numerical data Immunochemical protein assay Interlaboratory agreement Observer Variation Quality Control Reference material Reference Standards |
| title | Immunochemical assays of serum proteins: a European external quality assessment survey and the effects of calibration procedures on interlaboratory agreement |
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