Stimulation of lymphocyte migration by a novel low‐molecular weight compound in normal human skin and plasma
Normal human skin contains an extravascular population of lymphocytes which have been proposed to play a central role in immunosurveillance. The hypothesis that physiologic lymphocyte chemoattractants induce the migration of these cells has been tested by analysis of samples from normal human skin b...
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| Veröffentlicht in: | European journal of immunology 1990-03, Vol.20 (3), p.565-571 |
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| container_title | European journal of immunology |
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| creator | Bacon, Kevin B. Fincham, Nicholas J. Camp, Richard D. R. |
| description | Normal human skin contains an extravascular population of lymphocytes which have been proposed to play a central role in immunosurveillance. The hypothesis that physiologic lymphocyte chemoattractants induce the migration of these cells has been tested by analysis of samples from normal human skin by an in vitro lymphocyte migration assay in combination with high‐performance liquid chromatography (HPLC). Dilution‐related lymphocyte migration was seen on assay of aqueous extracts of stratum corneum, analysis after ultrafiltration consistently demonstrating activity in < 1‐kDa fractions. Similar results were obtained with chamber fluid from abraded normal skin and with normal plasma. A novel, polar, low‐molecular weight component in plasma and chamber fluid was purified to homogeneity by reverse‐phase HPLC. Material from the two sources possessed identical retention times on reverse‐phase HPLC and similar ultraviolet (UV) absorbance spectra (UV maximum 274 nm). The factor has been termed plasma‐associated lymphocyte chemoattractant (PALC). When purified to homogeneity, PALC induced concentration‐related lymphocyte migration, was extractable in lipid solvents and was not peptide in nature, as determined by mass spectrometric and amino acid analyses and proteolytic enzyme digestion. It is proposed that PALC may play a role in mediating physiologic margination, adherence and migration of lymphocytes in normal human skin. |
| doi_str_mv | 10.1002/eji.1830200316 |
| format | Article |
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R.</creator><creatorcontrib>Bacon, Kevin B. ; Fincham, Nicholas J. ; Camp, Richard D. R.</creatorcontrib><description>Normal human skin contains an extravascular population of lymphocytes which have been proposed to play a central role in immunosurveillance. The hypothesis that physiologic lymphocyte chemoattractants induce the migration of these cells has been tested by analysis of samples from normal human skin by an in vitro lymphocyte migration assay in combination with high‐performance liquid chromatography (HPLC). Dilution‐related lymphocyte migration was seen on assay of aqueous extracts of stratum corneum, analysis after ultrafiltration consistently demonstrating activity in < 1‐kDa fractions. Similar results were obtained with chamber fluid from abraded normal skin and with normal plasma. A novel, polar, low‐molecular weight component in plasma and chamber fluid was purified to homogeneity by reverse‐phase HPLC. Material from the two sources possessed identical retention times on reverse‐phase HPLC and similar ultraviolet (UV) absorbance spectra (UV maximum 274 nm). The factor has been termed plasma‐associated lymphocyte chemoattractant (PALC). When purified to homogeneity, PALC induced concentration‐related lymphocyte migration, was extractable in lipid solvents and was not peptide in nature, as determined by mass spectrometric and amino acid analyses and proteolytic enzyme digestion. It is proposed that PALC may play a role in mediating physiologic margination, adherence and migration of lymphocytes in normal human skin.</description><identifier>ISSN: 0014-2980</identifier><identifier>EISSN: 1521-4141</identifier><identifier>DOI: 10.1002/eji.1830200316</identifier><identifier>PMID: 2180725</identifier><identifier>CODEN: EJIMAF</identifier><language>eng</language><publisher>Weinheim: WILEY‐VCH Verlag GmbH</publisher><subject>Analysis of the immune response. 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R.</creatorcontrib><title>Stimulation of lymphocyte migration by a novel low‐molecular weight compound in normal human skin and plasma</title><title>European journal of immunology</title><addtitle>Eur J Immunol</addtitle><description>Normal human skin contains an extravascular population of lymphocytes which have been proposed to play a central role in immunosurveillance. The hypothesis that physiologic lymphocyte chemoattractants induce the migration of these cells has been tested by analysis of samples from normal human skin by an in vitro lymphocyte migration assay in combination with high‐performance liquid chromatography (HPLC). Dilution‐related lymphocyte migration was seen on assay of aqueous extracts of stratum corneum, analysis after ultrafiltration consistently demonstrating activity in < 1‐kDa fractions. Similar results were obtained with chamber fluid from abraded normal skin and with normal plasma. A novel, polar, low‐molecular weight component in plasma and chamber fluid was purified to homogeneity by reverse‐phase HPLC. Material from the two sources possessed identical retention times on reverse‐phase HPLC and similar ultraviolet (UV) absorbance spectra (UV maximum 274 nm). The factor has been termed plasma‐associated lymphocyte chemoattractant (PALC). When purified to homogeneity, PALC induced concentration‐related lymphocyte migration, was extractable in lipid solvents and was not peptide in nature, as determined by mass spectrometric and amino acid analyses and proteolytic enzyme digestion. It is proposed that PALC may play a role in mediating physiologic margination, adherence and migration of lymphocytes in normal human skin.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Biological and medical sciences</subject><subject>Biological Assay</subject><subject>Chemotactic Factors - blood</subject><subject>Chemotactic Factors - isolation & purification</subject><subject>Chemotaxis, Leukocyte</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>Lipids - analysis</subject><subject>Lymphocytes - physiology</subject><subject>Mass Spectrometry</subject><subject>Miscellaneous</subject><subject>Peptide Hydrolases - pharmacology</subject><subject>Regulatory factors and their cellular receptors</subject><subject>Skin Physiological Phenomena</subject><subject>Spectrophotometry, Ultraviolet</subject><issn>0014-2980</issn><issn>1521-4141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EKsvClRuSD4hblhnHTuIjqgotqsQBOEeO43Rd7DjYSVe58Qg8I09So10Vbj2NNP83_4zmJ-Q1wg4B2Htza3fYlMAASqyekA0KhgVHjk_JBgB5wWQDz8mLlG4BQFZCnpEzhg3UTGzI-HW2fnFqtmGkYaBu9dM-6HU21NubeOx3K1V0DHfGURcOf3799sEZnaciPRh7s5-pDn4Ky9hTO2YweuXofvFqpOlH7qgsTE4lr16SZ4Nyybw61S35_vHi2_llcf3l09X5h-tCc4CqGIRmg2x61XFlUMueQ2VMJ8qyl1yrDlE2QgAyVTdSGzRadEKKqpaq7EGzckveHX2nGH4uJs2tt0kb59RowpLaWlYSsMFHQRQ1COA8g7sjqGNIKZqhnaL1Kq4tQvs3iTYn0f5LIg-8OTkvnTf9A356fdbfnnSVtHJDVKO26QGrmqqU2W5L5BE7WGfWR5a2F5-v_jvhHm_Io7k</recordid><startdate>199003</startdate><enddate>199003</enddate><creator>Bacon, Kevin B.</creator><creator>Fincham, Nicholas J.</creator><creator>Camp, Richard D. R.</creator><general>WILEY‐VCH Verlag GmbH</general><general>Wiley-VCH</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199003</creationdate><title>Stimulation of lymphocyte migration by a novel low‐molecular weight compound in normal human skin and plasma</title><author>Bacon, Kevin B. ; Fincham, Nicholas J. ; Camp, Richard D. R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4006-f5c2f98dab4ae1c9d406eeb533d94cab119855012a789ce1ec5b595679a3d0c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Biological and medical sciences</topic><topic>Biological Assay</topic><topic>Chemotactic Factors - blood</topic><topic>Chemotactic Factors - isolation & purification</topic><topic>Chemotaxis, Leukocyte</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>Lipids - analysis</topic><topic>Lymphocytes - physiology</topic><topic>Mass Spectrometry</topic><topic>Miscellaneous</topic><topic>Peptide Hydrolases - pharmacology</topic><topic>Regulatory factors and their cellular receptors</topic><topic>Skin Physiological Phenomena</topic><topic>Spectrophotometry, Ultraviolet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bacon, Kevin B.</creatorcontrib><creatorcontrib>Fincham, Nicholas J.</creatorcontrib><creatorcontrib>Camp, Richard D. R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bacon, Kevin B.</au><au>Fincham, Nicholas J.</au><au>Camp, Richard D. R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stimulation of lymphocyte migration by a novel low‐molecular weight compound in normal human skin and plasma</atitle><jtitle>European journal of immunology</jtitle><addtitle>Eur J Immunol</addtitle><date>1990-03</date><risdate>1990</risdate><volume>20</volume><issue>3</issue><spage>565</spage><epage>571</epage><pages>565-571</pages><issn>0014-2980</issn><eissn>1521-4141</eissn><coden>EJIMAF</coden><abstract>Normal human skin contains an extravascular population of lymphocytes which have been proposed to play a central role in immunosurveillance. The hypothesis that physiologic lymphocyte chemoattractants induce the migration of these cells has been tested by analysis of samples from normal human skin by an in vitro lymphocyte migration assay in combination with high‐performance liquid chromatography (HPLC). Dilution‐related lymphocyte migration was seen on assay of aqueous extracts of stratum corneum, analysis after ultrafiltration consistently demonstrating activity in < 1‐kDa fractions. Similar results were obtained with chamber fluid from abraded normal skin and with normal plasma. A novel, polar, low‐molecular weight component in plasma and chamber fluid was purified to homogeneity by reverse‐phase HPLC. Material from the two sources possessed identical retention times on reverse‐phase HPLC and similar ultraviolet (UV) absorbance spectra (UV maximum 274 nm). The factor has been termed plasma‐associated lymphocyte chemoattractant (PALC). When purified to homogeneity, PALC induced concentration‐related lymphocyte migration, was extractable in lipid solvents and was not peptide in nature, as determined by mass spectrometric and amino acid analyses and proteolytic enzyme digestion. It is proposed that PALC may play a role in mediating physiologic margination, adherence and migration of lymphocytes in normal human skin.</abstract><cop>Weinheim</cop><pub>WILEY‐VCH Verlag GmbH</pub><pmid>2180725</pmid><doi>10.1002/eji.1830200316</doi><tpages>7</tpages></addata></record> |
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| ispartof | European journal of immunology, 1990-03, Vol.20 (3), p.565-571 |
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| language | eng |
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| subjects | Analysis of the immune response. Humoral and cellular immunity Biological and medical sciences Biological Assay Chemotactic Factors - blood Chemotactic Factors - isolation & purification Chemotaxis, Leukocyte Chromatography, High Pressure Liquid Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunobiology Lipids - analysis Lymphocytes - physiology Mass Spectrometry Miscellaneous Peptide Hydrolases - pharmacology Regulatory factors and their cellular receptors Skin Physiological Phenomena Spectrophotometry, Ultraviolet |
| title | Stimulation of lymphocyte migration by a novel low‐molecular weight compound in normal human skin and plasma |
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