Rapid detection of proteins by enzyme-linked immunofiltration assay after transfer onto nitrocellulose membranes

Enzyme‐linked immunofiltration assay (ELIFA) for labeling transferred proteins is an interesting and powerful technique for the rapid specific detection (15 min) of proteins immobilized on nitrocellulose or nylon membranes (0.20 and 0.45 μm). ELIFA does not require fastidious handling of the membran...

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