Production and characterization of human monoclonal antibodies against core protein p25 and transmembrane glycoprotein gp41 of HIV-1

With a view to obtaining human monoclonal antibodies against HIV-1 antigens we used the Epstein-Barr virus immortalization technique to induce lymphoblastoid cell lines from peripheral blood lymphocytes of 10 people who were seropositive for HIV-1 and had no clinical symptoms. A number of polyclonal...

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Veröffentlicht in:	AIDS (London) 1990-01, Vol.4 (1), p.11-19
Hauptverfasser:	Prigent, S, Goossens, D, Clerget-Raslain, B, Bahraoui, E, Roussel, M, Tsikas, G, Laurent, A, Montagnier, L, Salmon, C, Gluckman, J C
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Amino Acid Sequence Antibodies, Monoclonal - biosynthesis Antibody Specificity B-Lymphocytes - immunology Blotting, Western Cell Line, Transformed Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique Herpesvirus 4, Human - physiology HIV Antibodies - analysis HIV Antibodies - biosynthesis HIV Antibodies - immunology HIV Antigens - immunology HIV Envelope Protein gp41 - immunology HIV Seropositivity - immunology HIV-1 - immunology Humans Immunoglobulin G - biosynthesis Immunoglobulin G - immunology Molecular Sequence Data Radioimmunoprecipitation Assay Viral Core Proteins - immunology
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