Production and characterization of human monoclonal antibodies against core protein p25 and transmembrane glycoprotein gp41 of HIV-1
With a view to obtaining human monoclonal antibodies against HIV-1 antigens we used the Epstein-Barr virus immortalization technique to induce lymphoblastoid cell lines from peripheral blood lymphocytes of 10 people who were seropositive for HIV-1 and had no clinical symptoms. A number of polyclonal...
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| Veröffentlicht in: | AIDS (London) 1990-01, Vol.4 (1), p.11-19 |
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| container_title | AIDS (London) |
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| creator | Prigent, S Goossens, D Clerget-Raslain, B Bahraoui, E Roussel, M Tsikas, G Laurent, A Montagnier, L Salmon, C Gluckman, J C |
| description | With a view to obtaining human monoclonal antibodies against HIV-1 antigens we used the Epstein-Barr virus immortalization technique to induce lymphoblastoid cell lines from peripheral blood lymphocytes of 10 people who were seropositive for HIV-1 and had no clinical symptoms. A number of polyclonal lines were obtained which synthesized antibodies against most of the major proteins and glycoproteins of HIV-1. Three stable clones were characterized for class, secretion characteristics and specificity. Two of these clones produce antibodies which react with gp41, and the third reacts with p25. One of the anti-gp41 antibodies was found to have neutralizing activity. |
| doi_str_mv | 10.1097/00002030-199001000-00002 |
| format | Article |
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| identifier | ISSN: 0269-9370 |
| ispartof | AIDS (London), 1990-01, Vol.4 (1), p.11-19 |
| issn | 0269-9370 |
| language | eng |
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| source | MEDLINE; Journals@Ovid Ovid Autoload |
| subjects | AIDS/HIV Amino Acid Sequence Antibodies, Monoclonal - biosynthesis Antibody Specificity B-Lymphocytes - immunology Blotting, Western Cell Line, Transformed Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique Herpesvirus 4, Human - physiology HIV Antibodies - analysis HIV Antibodies - biosynthesis HIV Antibodies - immunology HIV Antigens - immunology HIV Envelope Protein gp41 - immunology HIV Seropositivity - immunology HIV-1 - immunology Humans Immunoglobulin G - biosynthesis Immunoglobulin G - immunology Molecular Sequence Data Radioimmunoprecipitation Assay Viral Core Proteins - immunology |
| title | Production and characterization of human monoclonal antibodies against core protein p25 and transmembrane glycoprotein gp41 of HIV-1 |
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