Purification and characterization of a 26‐kDa competitive inhibitor of interleukin 1
The urine of patients with fever above 39 °C contains an inhibitor of interleukin 1. Herein we describe the purification of the interleukin 1 inhibitor by ion‐exchange chromatography, hydrophobic chromatography, gel filtration and negative immunosorption. The purified protein has a molecular weight...
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| Veröffentlicht in: | European journal of immunology 1990-03, Vol.20 (3), p.683-689 |
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| container_title | European journal of immunology |
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| creator | Mazzei, Gonzalo J. Seckinger, Philippe L. Dayer, Jean‐Michel Shaw, Alan R. |
| description | The urine of patients with fever above 39 °C contains an inhibitor of interleukin 1. Herein we describe the purification of the interleukin 1 inhibitor by ion‐exchange chromatography, hydrophobic chromatography, gel filtration and negative immunosorption. The purified protein has a molecular weight of 26000; its size is reduced to 24000 upon treatment with endoglycosidase F. The IL 1 inhibitor is a competitive inhibitor and acts by binding to the IL 1 receptor. The inhibitor recognizes the murine and human IL 1 receptor with similar affinity. The purified IL 1 inhibitor has a specific activity of 3 × 107 to 4.5 × 107 units/mg as tested on the human astrocytoma (U‐373) cell proliferation bioassay and murine EL4.6.1 cell IL 1 receptor‐binding assay. |
| doi_str_mv | 10.1002/eji.1830200332 |
| format | Article |
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Herein we describe the purification of the interleukin 1 inhibitor by ion‐exchange chromatography, hydrophobic chromatography, gel filtration and negative immunosorption. The purified protein has a molecular weight of 26000; its size is reduced to 24000 upon treatment with endoglycosidase F. The IL 1 inhibitor is a competitive inhibitor and acts by binding to the IL 1 receptor. The inhibitor recognizes the murine and human IL 1 receptor with similar affinity. The purified IL 1 inhibitor has a specific activity of 3 × 107 to 4.5 × 107 units/mg as tested on the human astrocytoma (U‐373) cell proliferation bioassay and murine EL4.6.1 cell IL 1 receptor‐binding assay.</description><identifier>ISSN: 0014-2980</identifier><identifier>EISSN: 1521-4141</identifier><identifier>DOI: 10.1002/eji.1830200332</identifier><identifier>PMID: 2138562</identifier><identifier>CODEN: EJIMAF</identifier><language>eng</language><publisher>Weinheim: WILEY‐VCH Verlag GmbH</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Binding, Competitive ; Biological and medical sciences ; Chromatography ; Chromatography, High Pressure Liquid ; Fever - urine ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Glycoproteins - metabolism ; Glycoproteins - urine ; Glycosylation ; Humans ; Immunobiology ; Immunosorbent Techniques ; Interleukin-1 - antagonists & inhibitors ; Lymphocyte Activation ; Lymphokines, interleukins ( function, expression) ; Molecular Weight ; Receptors, Immunologic - metabolism ; Receptors, Interleukin-1 ; Regulatory factors and their cellular receptors</subject><ispartof>European journal of immunology, 1990-03, Vol.20 (3), p.683-689</ispartof><rights>Copyright © 1990 Wiley‐VCH Verlag GmbH & Co. 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Herein we describe the purification of the interleukin 1 inhibitor by ion‐exchange chromatography, hydrophobic chromatography, gel filtration and negative immunosorption. The purified protein has a molecular weight of 26000; its size is reduced to 24000 upon treatment with endoglycosidase F. The IL 1 inhibitor is a competitive inhibitor and acts by binding to the IL 1 receptor. The inhibitor recognizes the murine and human IL 1 receptor with similar affinity. The purified IL 1 inhibitor has a specific activity of 3 × 107 to 4.5 × 107 units/mg as tested on the human astrocytoma (U‐373) cell proliferation bioassay and murine EL4.6.1 cell IL 1 receptor‐binding assay.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Fever - urine</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Glycoproteins - metabolism</subject><subject>Glycoproteins - urine</subject><subject>Glycosylation</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>Immunosorbent Techniques</subject><subject>Interleukin-1 - antagonists & inhibitors</subject><subject>Lymphocyte Activation</subject><subject>Lymphokines, interleukins ( function, expression)</subject><subject>Molecular Weight</subject><subject>Receptors, Immunologic - metabolism</subject><subject>Receptors, Interleukin-1</subject><subject>Regulatory factors and their cellular receptors</subject><issn>0014-2980</issn><issn>1521-4141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL9OwzAQxi0EKqWwsiFlQGwp_pO49ohKgaJKMABrdHVs1W2aFDsBlYlH4Bl5EhIlArZOp7vvd9-dPoROCR4SjOmlXtohEQxTjBmje6hPYkrCiERkH_UxJlFIpcCH6Mj7JcZY8lj2UI8SJmJO--jlsXLWWAWlLfIA8jRQC3CgSu3sRzssTAAB5d-fX6trCFSx3ujSlvZNBzZf2LktC9cwNq93Ml2tbB6QY3RgIPP6pKsD9HwzeRrfhbOH2-n4ahaqqH4-ZExhrUXTpJwxQ9gICzoCwVNjFAXNqZxjNk8VGCqBUiEVBwWjSJmIUcEG6KL13bjitdK-TNbWK51lkOui8slIchEzwneCJOaS8ahxHLagcoX3Tptk4-wa3DYhOGkST-rEk7_E64Wzzrmar3X6i3cR1_p5p4NXkBkHubL-F-OCM8miGpMt9m4zvd1xNJncT_-98AMiZ5nN</recordid><startdate>199003</startdate><enddate>199003</enddate><creator>Mazzei, Gonzalo J.</creator><creator>Seckinger, Philippe L.</creator><creator>Dayer, Jean‐Michel</creator><creator>Shaw, Alan R.</creator><general>WILEY‐VCH Verlag GmbH</general><general>Wiley-VCH</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>199003</creationdate><title>Purification and characterization of a 26‐kDa competitive inhibitor of interleukin 1</title><author>Mazzei, Gonzalo J. ; Seckinger, Philippe L. ; Dayer, Jean‐Michel ; Shaw, Alan R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4002-33c0ee8c400d633f1370827a86dffc2ae629b03bdcaf29a2289c6aca74cf43283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Fever - urine</topic><topic>Fundamental and applied biological sciences. 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Herein we describe the purification of the interleukin 1 inhibitor by ion‐exchange chromatography, hydrophobic chromatography, gel filtration and negative immunosorption. The purified protein has a molecular weight of 26000; its size is reduced to 24000 upon treatment with endoglycosidase F. The IL 1 inhibitor is a competitive inhibitor and acts by binding to the IL 1 receptor. The inhibitor recognizes the murine and human IL 1 receptor with similar affinity. The purified IL 1 inhibitor has a specific activity of 3 × 107 to 4.5 × 107 units/mg as tested on the human astrocytoma (U‐373) cell proliferation bioassay and murine EL4.6.1 cell IL 1 receptor‐binding assay.</abstract><cop>Weinheim</cop><pub>WILEY‐VCH Verlag GmbH</pub><pmid>2138562</pmid><doi>10.1002/eji.1830200332</doi><tpages>7</tpages></addata></record> |
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| subjects | Analysis of the immune response. Humoral and cellular immunity Binding, Competitive Biological and medical sciences Chromatography Chromatography, High Pressure Liquid Fever - urine Fundamental and applied biological sciences. Psychology Fundamental immunology Glycoproteins - metabolism Glycoproteins - urine Glycosylation Humans Immunobiology Immunosorbent Techniques Interleukin-1 - antagonists & inhibitors Lymphocyte Activation Lymphokines, interleukins ( function, expression) Molecular Weight Receptors, Immunologic - metabolism Receptors, Interleukin-1 Regulatory factors and their cellular receptors |
| title | Purification and characterization of a 26‐kDa competitive inhibitor of interleukin 1 |
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