The Effect of Whole Ejaculate Filtration on the Morphology and the Fertility of Bovine Semen
A Sephadex G-15 filtration method was developed to remove abnormal and nonmotile bull sperm from an entire ejaculate. The efficiency of filtration was determined by adding freeze-killed sperm to the ejaculate or using ejaculates with elevated numbers of abnormal cells induced by scrotal insulation....
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| Veröffentlicht in: | Journal of dairy science 1990-01, Vol.73 (1), p.91-97 |
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| creator | Graham, E.F. Graham, J.K. |
| description | A Sephadex G-15 filtration method was developed to remove abnormal and nonmotile bull sperm from an entire ejaculate. The efficiency of filtration was determined by adding freeze-killed sperm to the ejaculate or using ejaculates with elevated numbers of abnormal cells induced by scrotal insulation. A fertility trial, using split ejaculates, compared fertility of filtered and unfiltered semen.
After filtration, samples with 0, 25, and 50% killed cells added contained 77 to 81% motile cells. Addition of 75% killed sperm resulted in significant decrease (52%) of motile cells following filtration. Morphologic examination of semen with elevated numbers of abnormal cells revealed higher percentages of sperm with normal shaped heads and normal or swollen acrosomes after filtration than in unfiltered samples. Percentage of pear-shaped heads, lifted acrosomes, and bent tails decreased after filtration. Six high fertility and six low fertility bulls were used to evaluate fertility of filtered semen. Filtering increased motile sperm from 51 to 57% and from 36 to 50% for the high and low fertility bulls, respectively. The 60- to 90-d nonreturn rates for high fertility bulls were not increased by filtering (73 vs. 72%). However, filtering significantly improved the nonreturn rates for the low fertility bulls (61 vs. 67%). |
| doi_str_mv | 10.3168/jds.S0022-0302(90)78650-X |
| format | Article |
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After filtration, samples with 0, 25, and 50% killed cells added contained 77 to 81% motile cells. Addition of 75% killed sperm resulted in significant decrease (52%) of motile cells following filtration. Morphologic examination of semen with elevated numbers of abnormal cells revealed higher percentages of sperm with normal shaped heads and normal or swollen acrosomes after filtration than in unfiltered samples. Percentage of pear-shaped heads, lifted acrosomes, and bent tails decreased after filtration. Six high fertility and six low fertility bulls were used to evaluate fertility of filtered semen. Filtering increased motile sperm from 51 to 57% and from 36 to 50% for the high and low fertility bulls, respectively. The 60- to 90-d nonreturn rates for high fertility bulls were not increased by filtering (73 vs. 72%). However, filtering significantly improved the nonreturn rates for the low fertility bulls (61 vs. 67%).</description><identifier>ISSN: 0022-0302</identifier><identifier>EISSN: 1525-3198</identifier><identifier>DOI: 10.3168/jds.S0022-0302(90)78650-X</identifier><identifier>PMID: 2312886</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>anatomia animal ; anatomie animale ; animal anatomy ; Animals ; bulls ; Cattle - physiology ; Chromatography, Gel ; Cryopreservation ; fertilidad ; fertilite ; Fertility ; filtracion ; filtration ; Male ; semen ; Semen - physiology ; Sperm Motility ; spermatozoa ; Spermatozoa - abnormalities ; sperme ; taureau ; toro</subject><ispartof>Journal of dairy science, 1990-01, Vol.73 (1), p.91-97</ispartof><rights>1990 American Dairy Science Association</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-a72baf863b614b452bf11774d158f563d009c6d62ffc7754b68bdb2f8e3e2db13</citedby><cites>FETCH-LOGICAL-c503t-a72baf863b614b452bf11774d158f563d009c6d62ffc7754b68bdb2f8e3e2db13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.3168/jds.S0022-0302(90)78650-X$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,4024,27869,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2312886$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Graham, E.F.</creatorcontrib><creatorcontrib>Graham, J.K.</creatorcontrib><creatorcontrib>University of Minnesota, St. Paul</creatorcontrib><title>The Effect of Whole Ejaculate Filtration on the Morphology and the Fertility of Bovine Semen</title><title>Journal of dairy science</title><addtitle>J Dairy Sci</addtitle><description>A Sephadex G-15 filtration method was developed to remove abnormal and nonmotile bull sperm from an entire ejaculate. The efficiency of filtration was determined by adding freeze-killed sperm to the ejaculate or using ejaculates with elevated numbers of abnormal cells induced by scrotal insulation. A fertility trial, using split ejaculates, compared fertility of filtered and unfiltered semen.
After filtration, samples with 0, 25, and 50% killed cells added contained 77 to 81% motile cells. Addition of 75% killed sperm resulted in significant decrease (52%) of motile cells following filtration. Morphologic examination of semen with elevated numbers of abnormal cells revealed higher percentages of sperm with normal shaped heads and normal or swollen acrosomes after filtration than in unfiltered samples. Percentage of pear-shaped heads, lifted acrosomes, and bent tails decreased after filtration. Six high fertility and six low fertility bulls were used to evaluate fertility of filtered semen. Filtering increased motile sperm from 51 to 57% and from 36 to 50% for the high and low fertility bulls, respectively. The 60- to 90-d nonreturn rates for high fertility bulls were not increased by filtering (73 vs. 72%). However, filtering significantly improved the nonreturn rates for the low fertility bulls (61 vs. 67%).</description><subject>anatomia animal</subject><subject>anatomie animale</subject><subject>animal anatomy</subject><subject>Animals</subject><subject>bulls</subject><subject>Cattle - physiology</subject><subject>Chromatography, Gel</subject><subject>Cryopreservation</subject><subject>fertilidad</subject><subject>fertilite</subject><subject>Fertility</subject><subject>filtracion</subject><subject>filtration</subject><subject>Male</subject><subject>semen</subject><subject>Semen - physiology</subject><subject>Sperm Motility</subject><subject>spermatozoa</subject><subject>Spermatozoa - abnormalities</subject><subject>sperme</subject><subject>taureau</subject><subject>toro</subject><issn>0022-0302</issn><issn>1525-3198</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>K30</sourceid><recordid>eNqNkEtvEzEURi0EKmnhJ4AGUSFYTPEj48cSogaQilikFV0gWR4_EkeTcbA9Rfn3eDJRF6yQLFn2Pffz9QHgDYJXBFH-cWvS1QpCjGtIIH4v4AfGaQPr-ydghhrc1AQJ_hTMHpHn4DylbTkiDJszcIYJwpzTGfh1u7HVtXNW5yq46ucmdOW8VXroVLbV0nc5quxDX5WVC_s9xH2BwvpQqd4cr5Y2Zt_5fBgTPocH39tqZXe2fwGeOdUl-_K0X4C75fXt4mt98-PLt8Wnm1o3kORaMdwqxylpKZq38wa3DiHG5gY13DWUGAiFpoZi5zRjzbylvDUtdtwSi02LyAV4N-XuY_g92JTlzidtu071NgxJMkEZpgQX8O0_4DYMsS-zScQZg5RjJAolJkrHkFK0Tu6j36l4kAjK0b8s_uXRvxzlSgHl0b-8L72vTi8M7c6ax86T8FK_nOobv9788dHKtFNdV2g0pjIikRTjj15PmFNBqnX0Sd6tkBAQQs4oH2dcTIAtXh-8jTJpb3ttTcnUWZrg_2Pav2E0rOo</recordid><startdate>199001</startdate><enddate>199001</enddate><creator>Graham, E.F.</creator><creator>Graham, J.K.</creator><general>Elsevier Inc</general><general>Am Dairy Sci Assoc</general><general>American Dairy Science Association</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7WH</scope><scope>K30</scope><scope>PAAUG</scope><scope>PAWHS</scope><scope>PAWZZ</scope><scope>PAXOH</scope><scope>PBHAV</scope><scope>PBQSW</scope><scope>PBYQZ</scope><scope>PCIWU</scope><scope>PCMID</scope><scope>PCZJX</scope><scope>PDGRG</scope><scope>PDWWI</scope><scope>PETMR</scope><scope>PFVGT</scope><scope>PGXDX</scope><scope>PIHIL</scope><scope>PISVA</scope><scope>PJCTQ</scope><scope>PJTMS</scope><scope>PLCHJ</scope><scope>PMHAD</scope><scope>PNQDJ</scope><scope>POUND</scope><scope>PPLAD</scope><scope>PQAPC</scope><scope>PQCAN</scope><scope>PQCMW</scope><scope>PQEME</scope><scope>PQHKH</scope><scope>PQMID</scope><scope>PQNCT</scope><scope>PQNET</scope><scope>PQSCT</scope><scope>PQSET</scope><scope>PSVJG</scope><scope>PVMQY</scope><scope>PZGFC</scope><scope>7X8</scope></search><sort><creationdate>199001</creationdate><title>The Effect of Whole Ejaculate Filtration on the Morphology and the Fertility of Bovine Semen</title><author>Graham, E.F. ; Graham, J.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-a72baf863b614b452bf11774d158f563d009c6d62ffc7754b68bdb2f8e3e2db13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>anatomia animal</topic><topic>anatomie animale</topic><topic>animal anatomy</topic><topic>Animals</topic><topic>bulls</topic><topic>Cattle - physiology</topic><topic>Chromatography, Gel</topic><topic>Cryopreservation</topic><topic>fertilidad</topic><topic>fertilite</topic><topic>Fertility</topic><topic>filtracion</topic><topic>filtration</topic><topic>Male</topic><topic>semen</topic><topic>Semen - physiology</topic><topic>Sperm Motility</topic><topic>spermatozoa</topic><topic>Spermatozoa - abnormalities</topic><topic>sperme</topic><topic>taureau</topic><topic>toro</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Graham, E.F.</creatorcontrib><creatorcontrib>Graham, J.K.</creatorcontrib><creatorcontrib>University of Minnesota, St. Paul</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Periodicals Index Online Segment 50</collection><collection>Periodicals Index Online</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - 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The efficiency of filtration was determined by adding freeze-killed sperm to the ejaculate or using ejaculates with elevated numbers of abnormal cells induced by scrotal insulation. A fertility trial, using split ejaculates, compared fertility of filtered and unfiltered semen.
After filtration, samples with 0, 25, and 50% killed cells added contained 77 to 81% motile cells. Addition of 75% killed sperm resulted in significant decrease (52%) of motile cells following filtration. Morphologic examination of semen with elevated numbers of abnormal cells revealed higher percentages of sperm with normal shaped heads and normal or swollen acrosomes after filtration than in unfiltered samples. Percentage of pear-shaped heads, lifted acrosomes, and bent tails decreased after filtration. Six high fertility and six low fertility bulls were used to evaluate fertility of filtered semen. Filtering increased motile sperm from 51 to 57% and from 36 to 50% for the high and low fertility bulls, respectively. The 60- to 90-d nonreturn rates for high fertility bulls were not increased by filtering (73 vs. 72%). However, filtering significantly improved the nonreturn rates for the low fertility bulls (61 vs. 67%).</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>2312886</pmid><doi>10.3168/jds.S0022-0302(90)78650-X</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of dairy science, 1990-01, Vol.73 (1), p.91-97 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB Electronic Journals Library; Periodicals Index Online |
| subjects | anatomia animal anatomie animale animal anatomy Animals bulls Cattle - physiology Chromatography, Gel Cryopreservation fertilidad fertilite Fertility filtracion filtration Male semen Semen - physiology Sperm Motility spermatozoa Spermatozoa - abnormalities sperme taureau toro |
| title | The Effect of Whole Ejaculate Filtration on the Morphology and the Fertility of Bovine Semen |
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