Functional evidence for UDP-galactose transporter in Saccharomyces cerevisiae through the in vivo galactosylation and in vitro transport assay

The oligosaccharide profiles in glycoproteins are determined by a series of processing reactions catalyzed by Golgi glycosyltransferases and glycosidases. Recently in vivo galactose incorporation in Saccharomyces cerevisiae has been demonstrated through the expression of human beta-1,4-galactosyltra...

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Veröffentlicht in:	The Journal of biological chemistry 1998-01, Vol.273 (5), p.2583-2590
Hauptverfasser:	Roy, S.K, Yoko-o, T, Ikenaga, H, Jigami, Y
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVE TRANSPORT ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE BINDING PROTEINS BIOCHEMISTRY BIOCHIMIE Biological Transport BIOQUIMICA CELL MEMBRANES CYTOCHEMISTRY ENZYMIC ACTIVITY FOSFATOS (ESTERES) GALACTOMANNANS GALACTOSA GALACTOSE Galactose - metabolism GALACTOSYLTRANSFERASE Galactosyltransferases - genetics Galactosyltransferases - metabolism GDP-MANNOSE GLICOSILTRANSFERASAS GLYCOSYLTRANSFERASE GLYCOSYLTRANSFERASES Golgi Apparatus - enzymology Guanosine Diphosphate Mannose - metabolism MANANOS MANNANE MANNANS MANNOSE MANOSA MEMBRANAS CELULARES MEMBRANE CELLULAIRE Monosaccharide Transport Proteins - metabolism MUTANT MUTANTES MUTANTS Mutation NUCLEOTIDE NUCLEOTIDES NUCLEOTIDOS PHOSPHATE (ESTER) PHOSPHATES (ESTERS) PLASMA MEMBRANES PROTEINAS AGLUTINANTES PROTEINE DE LIAISON Saccharomyces - enzymology Saccharomyces - physiology SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - physiology SCHIZOSACCHAROMYCES POMBE Species Specificity Uridine Diphosphate Galactose - metabolism
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