Trophic and tropic effects of striatal astrocytes on cografted mesencephalic dopamine neurons and their axons

Astrocytes from the ventral mesencephalon and from the striatum respectively promote the dendritic and axonal arborization of dopamine (DA) neurons in vitro. To test this response in vivo, astrocytes in primary cultures from the neonatal cerebral cortex, ventral mesencephalon, or striatum were coimp...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of neuroscience research 1998-01, Vol.51 (1), p.23-40
Hauptverfasser: Pierret, P., Quenneville, N., Vandaele, S., Abbaszadeh, R., Doucet, G., Lanctǒt, C., Crine, P.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 40
container_issue 1
container_start_page 23
container_title Journal of neuroscience research
container_volume 51
creator Pierret, P.
Quenneville, N.
Vandaele, S.
Abbaszadeh, R.
Doucet, G.
Lanctǒt, C.
Crine, P.
description Astrocytes from the ventral mesencephalon and from the striatum respectively promote the dendritic and axonal arborization of dopamine (DA) neurons in vitro. To test this response in vivo, astrocytes in primary cultures from the neonatal cerebral cortex, ventral mesencephalon, or striatum were coimplanted with fetal ventral mesencephalic tissue into the intact or DA‐denervated striatum of adult rats and these cografts examined after 3–6 months by tyrosine hydroxylase (TH) immunohistochemistry (intact recipients) or after 5–6 months by in vitro [H]DA‐uptake autoradiography (DA‐denervated recipients). In contrast with single ventral mesencephalic grafts, all types of cograft displayed a rather uniform distribution of TH‐immunoreactive perikarya. The average size of TH‐immunoreactive cell bodies was not significantly different in cografts containing cortical or mesencephalic astrocytes and in single ventral mesencephalic grafts, but it was significantly larger in cografts containing striatal astrocytes. Nevertheless, the number of [H]DA‐labeled terminals in the DA‐lesioned host striatum was clearly smaller with cografts of striatal astrocytes than with single mesencephalic grafts or with cografts containing cortical astrocytes. On the other hand, cografts of striatal astrocytes contained much higher numbers of [H]DA‐labeled terminals than the other types of graft or cograft. Thus, while cografted astrocytes in general influence the distribution of DA neurons within the graft, astrocytes from the neonatal striatum have a trophic effect on DA perikarya and a tropic effect on DA axons, keeping the latter within the graft.
doi_str_mv 10.1002/(SICI)1097-4547(19980101)51:1<23::AID-JNR3>3.0.CO;2-N
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_79669456</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79669456</sourcerecordid><originalsourceid>FETCH-LOGICAL-i2763-fb123890d09ab6c73a6f1d2d4473f04a15539e21cd5dc5e0a77792ada5eff4ef3</originalsourceid><addsrcrecordid>eNqFkVtvEzEQhS0EKiHwE5D2CbUPG8b3OlykKpAS1CZSKPQJjZy1lyzsjfVGNP8erxLyypPPeM4cW_MR8o7ChAKw1-dfFrPFBQWjUyGFPqfGXAIFeiHplL5lfDq9WnxIPy_X_D2fwGS2esPS5SMyOk08JiPgClIBlD0lz0L4CQDGSH5GzoyQjIMakequa9ptkSW2dkkfdZQ-z33Wh6TJk9B3he1tmdiommzf-3hdJ1nzo7N5711S-eDrzLdbW8ZJ17S2Kmqf1H7XNXU4pG590SX2IdbPyZPclsG_OJ5j8nX-8W72Kb1ZXS9mVzdpwbTiab6hjF8acGDsRmWaW5VTx5wQmucgLJWSG89o5qTLpAertTbMOivj14XP-Zi8OuS2XfN750OPVREyX5a29s0uoDZKxRWo_xqpElqY-NyYvDwad5vKO2y7orLdHo-LjP3vh_6fovT7U5sCDjBxYIkDGRzI4D-WKClSZBwjShxQIkfA2QoZLodaGJDRBjzmp4f8IvT-4ZRvu1-oNNcS75fXeLue336b30tc87_ZeKmZ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16474955</pqid></control><display><type>article</type><title>Trophic and tropic effects of striatal astrocytes on cografted mesencephalic dopamine neurons and their axons</title><source>MEDLINE</source><source>Wiley Online Library</source><creator>Pierret, P. ; Quenneville, N. ; Vandaele, S. ; Abbaszadeh, R. ; Doucet, G. ; Lanctǒt, C. ; Crine, P.</creator><creatorcontrib>Pierret, P. ; Quenneville, N. ; Vandaele, S. ; Abbaszadeh, R. ; Doucet, G. ; Lanctǒt, C. ; Crine, P.</creatorcontrib><description>Astrocytes from the ventral mesencephalon and from the striatum respectively promote the dendritic and axonal arborization of dopamine (DA) neurons in vitro. To test this response in vivo, astrocytes in primary cultures from the neonatal cerebral cortex, ventral mesencephalon, or striatum were coimplanted with fetal ventral mesencephalic tissue into the intact or DA‐denervated striatum of adult rats and these cografts examined after 3–6 months by tyrosine hydroxylase (TH) immunohistochemistry (intact recipients) or after 5–6 months by in vitro [H]DA‐uptake autoradiography (DA‐denervated recipients). In contrast with single ventral mesencephalic grafts, all types of cograft displayed a rather uniform distribution of TH‐immunoreactive perikarya. The average size of TH‐immunoreactive cell bodies was not significantly different in cografts containing cortical or mesencephalic astrocytes and in single ventral mesencephalic grafts, but it was significantly larger in cografts containing striatal astrocytes. Nevertheless, the number of [H]DA‐labeled terminals in the DA‐lesioned host striatum was clearly smaller with cografts of striatal astrocytes than with single mesencephalic grafts or with cografts containing cortical astrocytes. On the other hand, cografts of striatal astrocytes contained much higher numbers of [H]DA‐labeled terminals than the other types of graft or cograft. Thus, while cografted astrocytes in general influence the distribution of DA neurons within the graft, astrocytes from the neonatal striatum have a trophic effect on DA perikarya and a tropic effect on DA axons, keeping the latter within the graft.</description><identifier>ISSN: 0360-4012</identifier><identifier>EISSN: 1097-4547</identifier><identifier>DOI: 10.1002/(SICI)1097-4547(19980101)51:1&lt;23::AID-JNR3&gt;3.0.CO;2-N</identifier><identifier>PMID: 9452306</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Astrocytes - chemistry ; Astrocytes - transplantation ; Autoradiography ; Axons - transplantation ; Cells, Cultured ; Corpus Striatum - cytology ; Corpus Striatum - transplantation ; Denervation ; Dopamine - analysis ; Mesencephalon - transplantation ; Nerve Regeneration - physiology ; neural transplantation ; Neurons - chemistry ; Neurons - transplantation ; Neurons - ultrastructure ; Oxidopamine ; Parkinson's disease ; primary culture ; Rats</subject><ispartof>Journal of neuroscience research, 1998-01, Vol.51 (1), p.23-40</ispartof><rights>Copyright © 1998 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291097-4547%2819980101%2951%3A1%3C23%3A%3AAID-JNR3%3E3.0.CO%3B2-N$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291097-4547%2819980101%2951%3A1%3C23%3A%3AAID-JNR3%3E3.0.CO%3B2-N$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9452306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pierret, P.</creatorcontrib><creatorcontrib>Quenneville, N.</creatorcontrib><creatorcontrib>Vandaele, S.</creatorcontrib><creatorcontrib>Abbaszadeh, R.</creatorcontrib><creatorcontrib>Doucet, G.</creatorcontrib><creatorcontrib>Lanctǒt, C.</creatorcontrib><creatorcontrib>Crine, P.</creatorcontrib><title>Trophic and tropic effects of striatal astrocytes on cografted mesencephalic dopamine neurons and their axons</title><title>Journal of neuroscience research</title><addtitle>J. Neurosci. Res</addtitle><description>Astrocytes from the ventral mesencephalon and from the striatum respectively promote the dendritic and axonal arborization of dopamine (DA) neurons in vitro. To test this response in vivo, astrocytes in primary cultures from the neonatal cerebral cortex, ventral mesencephalon, or striatum were coimplanted with fetal ventral mesencephalic tissue into the intact or DA‐denervated striatum of adult rats and these cografts examined after 3–6 months by tyrosine hydroxylase (TH) immunohistochemistry (intact recipients) or after 5–6 months by in vitro [H]DA‐uptake autoradiography (DA‐denervated recipients). In contrast with single ventral mesencephalic grafts, all types of cograft displayed a rather uniform distribution of TH‐immunoreactive perikarya. The average size of TH‐immunoreactive cell bodies was not significantly different in cografts containing cortical or mesencephalic astrocytes and in single ventral mesencephalic grafts, but it was significantly larger in cografts containing striatal astrocytes. Nevertheless, the number of [H]DA‐labeled terminals in the DA‐lesioned host striatum was clearly smaller with cografts of striatal astrocytes than with single mesencephalic grafts or with cografts containing cortical astrocytes. On the other hand, cografts of striatal astrocytes contained much higher numbers of [H]DA‐labeled terminals than the other types of graft or cograft. Thus, while cografted astrocytes in general influence the distribution of DA neurons within the graft, astrocytes from the neonatal striatum have a trophic effect on DA perikarya and a tropic effect on DA axons, keeping the latter within the graft.</description><subject>Animals</subject><subject>Astrocytes - chemistry</subject><subject>Astrocytes - transplantation</subject><subject>Autoradiography</subject><subject>Axons - transplantation</subject><subject>Cells, Cultured</subject><subject>Corpus Striatum - cytology</subject><subject>Corpus Striatum - transplantation</subject><subject>Denervation</subject><subject>Dopamine - analysis</subject><subject>Mesencephalon - transplantation</subject><subject>Nerve Regeneration - physiology</subject><subject>neural transplantation</subject><subject>Neurons - chemistry</subject><subject>Neurons - transplantation</subject><subject>Neurons - ultrastructure</subject><subject>Oxidopamine</subject><subject>Parkinson's disease</subject><subject>primary culture</subject><subject>Rats</subject><issn>0360-4012</issn><issn>1097-4547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVtvEzEQhS0EKiHwE5D2CbUPG8b3OlykKpAS1CZSKPQJjZy1lyzsjfVGNP8erxLyypPPeM4cW_MR8o7ChAKw1-dfFrPFBQWjUyGFPqfGXAIFeiHplL5lfDq9WnxIPy_X_D2fwGS2esPS5SMyOk08JiPgClIBlD0lz0L4CQDGSH5GzoyQjIMakequa9ptkSW2dkkfdZQ-z33Wh6TJk9B3he1tmdiommzf-3hdJ1nzo7N5711S-eDrzLdbW8ZJ17S2Kmqf1H7XNXU4pG590SX2IdbPyZPclsG_OJ5j8nX-8W72Kb1ZXS9mVzdpwbTiab6hjF8acGDsRmWaW5VTx5wQmucgLJWSG89o5qTLpAertTbMOivj14XP-Zi8OuS2XfN750OPVREyX5a29s0uoDZKxRWo_xqpElqY-NyYvDwad5vKO2y7orLdHo-LjP3vh_6fovT7U5sCDjBxYIkDGRzI4D-WKClSZBwjShxQIkfA2QoZLodaGJDRBjzmp4f8IvT-4ZRvu1-oNNcS75fXeLue336b30tc87_ZeKmZ</recordid><startdate>199801</startdate><enddate>199801</enddate><creator>Pierret, P.</creator><creator>Quenneville, N.</creator><creator>Vandaele, S.</creator><creator>Abbaszadeh, R.</creator><creator>Doucet, G.</creator><creator>Lanctǒt, C.</creator><creator>Crine, P.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>199801</creationdate><title>Trophic and tropic effects of striatal astrocytes on cografted mesencephalic dopamine neurons and their axons</title><author>Pierret, P. ; Quenneville, N. ; Vandaele, S. ; Abbaszadeh, R. ; Doucet, G. ; Lanctǒt, C. ; Crine, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i2763-fb123890d09ab6c73a6f1d2d4473f04a15539e21cd5dc5e0a77792ada5eff4ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Astrocytes - chemistry</topic><topic>Astrocytes - transplantation</topic><topic>Autoradiography</topic><topic>Axons - transplantation</topic><topic>Cells, Cultured</topic><topic>Corpus Striatum - cytology</topic><topic>Corpus Striatum - transplantation</topic><topic>Denervation</topic><topic>Dopamine - analysis</topic><topic>Mesencephalon - transplantation</topic><topic>Nerve Regeneration - physiology</topic><topic>neural transplantation</topic><topic>Neurons - chemistry</topic><topic>Neurons - transplantation</topic><topic>Neurons - ultrastructure</topic><topic>Oxidopamine</topic><topic>Parkinson's disease</topic><topic>primary culture</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pierret, P.</creatorcontrib><creatorcontrib>Quenneville, N.</creatorcontrib><creatorcontrib>Vandaele, S.</creatorcontrib><creatorcontrib>Abbaszadeh, R.</creatorcontrib><creatorcontrib>Doucet, G.</creatorcontrib><creatorcontrib>Lanctǒt, C.</creatorcontrib><creatorcontrib>Crine, P.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pierret, P.</au><au>Quenneville, N.</au><au>Vandaele, S.</au><au>Abbaszadeh, R.</au><au>Doucet, G.</au><au>Lanctǒt, C.</au><au>Crine, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Trophic and tropic effects of striatal astrocytes on cografted mesencephalic dopamine neurons and their axons</atitle><jtitle>Journal of neuroscience research</jtitle><addtitle>J. Neurosci. Res</addtitle><date>1998-01</date><risdate>1998</risdate><volume>51</volume><issue>1</issue><spage>23</spage><epage>40</epage><pages>23-40</pages><issn>0360-4012</issn><eissn>1097-4547</eissn><abstract>Astrocytes from the ventral mesencephalon and from the striatum respectively promote the dendritic and axonal arborization of dopamine (DA) neurons in vitro. To test this response in vivo, astrocytes in primary cultures from the neonatal cerebral cortex, ventral mesencephalon, or striatum were coimplanted with fetal ventral mesencephalic tissue into the intact or DA‐denervated striatum of adult rats and these cografts examined after 3–6 months by tyrosine hydroxylase (TH) immunohistochemistry (intact recipients) or after 5–6 months by in vitro [H]DA‐uptake autoradiography (DA‐denervated recipients). In contrast with single ventral mesencephalic grafts, all types of cograft displayed a rather uniform distribution of TH‐immunoreactive perikarya. The average size of TH‐immunoreactive cell bodies was not significantly different in cografts containing cortical or mesencephalic astrocytes and in single ventral mesencephalic grafts, but it was significantly larger in cografts containing striatal astrocytes. Nevertheless, the number of [H]DA‐labeled terminals in the DA‐lesioned host striatum was clearly smaller with cografts of striatal astrocytes than with single mesencephalic grafts or with cografts containing cortical astrocytes. On the other hand, cografts of striatal astrocytes contained much higher numbers of [H]DA‐labeled terminals than the other types of graft or cograft. Thus, while cografted astrocytes in general influence the distribution of DA neurons within the graft, astrocytes from the neonatal striatum have a trophic effect on DA perikarya and a tropic effect on DA axons, keeping the latter within the graft.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>9452306</pmid><doi>10.1002/(SICI)1097-4547(19980101)51:1&lt;23::AID-JNR3&gt;3.0.CO;2-N</doi><tpages>18</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0360-4012
ispartof Journal of neuroscience research, 1998-01, Vol.51 (1), p.23-40
issn 0360-4012
1097-4547
language eng
recordid cdi_proquest_miscellaneous_79669456
source MEDLINE; Wiley Online Library
subjects Animals
Astrocytes - chemistry
Astrocytes - transplantation
Autoradiography
Axons - transplantation
Cells, Cultured
Corpus Striatum - cytology
Corpus Striatum - transplantation
Denervation
Dopamine - analysis
Mesencephalon - transplantation
Nerve Regeneration - physiology
neural transplantation
Neurons - chemistry
Neurons - transplantation
Neurons - ultrastructure
Oxidopamine
Parkinson's disease
primary culture
Rats
title Trophic and tropic effects of striatal astrocytes on cografted mesencephalic dopamine neurons and their axons
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T12%3A41%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Trophic%20and%20tropic%20effects%20of%20striatal%20astrocytes%20on%20cografted%20mesencephalic%20dopamine%20neurons%20and%20their%20axons&rft.jtitle=Journal%20of%20neuroscience%20research&rft.au=Pierret,%20P.&rft.date=1998-01&rft.volume=51&rft.issue=1&rft.spage=23&rft.epage=40&rft.pages=23-40&rft.issn=0360-4012&rft.eissn=1097-4547&rft_id=info:doi/10.1002/(SICI)1097-4547(19980101)51:1%3C23::AID-JNR3%3E3.0.CO;2-N&rft_dat=%3Cproquest_pubme%3E79669456%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16474955&rft_id=info:pmid/9452306&rfr_iscdi=true