Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays

We have used high-density oligonucleotide probe arrays (chips) for bacterial transcript imaging. We designed a chip containing probes representing 106 Hemophilus influenzae genes and 100 Streptococcus pneumoniae genes. The apparent lack of polyadenylated transcripts excludes enrichment of mRNA by af...

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Veröffentlicht in:	Nature biotechnology 1998-01, Vol.16 (1), p.45-48
Hauptverfasser:	Saizieu, Antoine de, Certa, Ulrich, Warrington, Janet, Gray, Christopher, Keck, Wolfgang, Mous, Jan
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biosensors Biotechnology Biotinylation Blotting, Northern Fluorescent Dyes Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genome, Bacterial Haemophilus influenzae - genetics Life Sciences Methods. Procedures. Technologies Miscellaneous Nucleic Acid Hybridization Oligonucleotide Probes research-article RNA, Bacterial Streptococcus pneumoniae - genetics Synthetic digonucleotides and genes. Sequencing Transcription, Genetic - genetics Various methods and equipments
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