Detection of APC mutations in stool DNA of patients with colorectal cancer by HD-PCR

In most cases the analysis of DNA mutations in presence of a high excess of wild type DNA fail because of the low sensitivity of the performed method for mutation detection. Here we describe the new high‐sensitive and non‐radioactive HD‐PCR method (for HeteroDuplex‐PCR). In opposite to the conventio...

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Veröffentlicht in:	Human mutation 1998, Vol.11 (1), p.84-89
Hauptverfasser:	Deuter, Rainer, Müller, Oliver
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoma - genetics Adenomatous Polyposis Coli Protein Colorectal cancer Colorectal Neoplasms - genetics Cytoskeletal Proteins - genetics DNA Mutational Analysis DNA, Neoplasm - analysis Feces - chemistry Genes, APC Humans Mutation Nucleic Acid Heteroduplexes Polymerase Chain Reaction - methods Sensitivity and Specificity
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