Targeted disruption of the mouse villin gene does not impair the morphogenesis of microvilli

The small intestine is functionally dependent on the presence of the brush border, a tightly packed array of microvilli that forms the amplified apical surface of absorptive cells. In the core of each microvillus, actin filaments are bundled by two proteins, villin and fimbrin. Previous in vitro stu...

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Veröffentlicht in:Developmental dynamics 1998-01, Vol.211 (1), p.109-121
Hauptverfasser: Pinson, Kathleen I., Dunbar, Laura, Samuelson, Linda, Gumucio, Deborah L.
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container_title Developmental dynamics
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creator Pinson, Kathleen I.
Dunbar, Laura
Samuelson, Linda
Gumucio, Deborah L.
description The small intestine is functionally dependent on the presence of the brush border, a tightly packed array of microvilli that forms the amplified apical surface of absorptive cells. In the core of each microvillus, actin filaments are bundled by two proteins, villin and fimbrin. Previous in vitro studies using antisense approaches indicated that villin plays an important role in the morphogenesis of microvilli. To examine the in vivo consequences of villin deficiency, we disrupted the mouse villin gene by targeted recombination in mouse embryonic stem cells. A β‐galactosidase cDNA was also introduced into the villin locus by the targeting event. Homozygous villin‐deficient mice are viable, fertile, and display no gross abnormalities. Intact microvilli are present in the small intestine, colon, kidney proximal tubules, and liver bile canaliculi. Although subtle ultrastructural abnormalities can be detected in the actin cores of small intestinal microvilli, localization of sucrase isomaltase, brush border myosin I, and zonula occludens I to the microvillar surface of the small intestine is normal. Thus, in vivo, villin plays a minor or redundant role in the generation of microvilli in multiple absorptive tissues. Dev. Dyn. 1998;211:109–121. © 1997 Wiley‐Liss, Inc.
doi_str_mv 10.1002/(SICI)1097-0177(199801)211:1<109::AID-AJA10>3.0.CO;2-7
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In the core of each microvillus, actin filaments are bundled by two proteins, villin and fimbrin. Previous in vitro studies using antisense approaches indicated that villin plays an important role in the morphogenesis of microvilli. To examine the in vivo consequences of villin deficiency, we disrupted the mouse villin gene by targeted recombination in mouse embryonic stem cells. A β‐galactosidase cDNA was also introduced into the villin locus by the targeting event. Homozygous villin‐deficient mice are viable, fertile, and display no gross abnormalities. Intact microvilli are present in the small intestine, colon, kidney proximal tubules, and liver bile canaliculi. Although subtle ultrastructural abnormalities can be detected in the actin cores of small intestinal microvilli, localization of sucrase isomaltase, brush border myosin I, and zonula occludens I to the microvillar surface of the small intestine is normal. 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Dyn. 1998;211:109–121. © 1997 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>brush border development</subject><subject>Carrier Proteins - analysis</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - physiology</subject><subject>embryonic stem cells</subject><subject>Epithelial Cells - ultrastructure</subject><subject>Female</subject><subject>Gene Targeting</subject><subject>Genes - physiology</subject><subject>homologous recombination</subject><subject>Intestine, Small - chemistry</subject><subject>Intestine, Small - embryology</subject><subject>Intestine, Small - ultrastructure</subject><subject>Male</subject><subject>Membrane Proteins - analysis</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Microfilament Proteins - analysis</subject><subject>Microfilament Proteins - genetics</subject><subject>Microfilament Proteins - physiology</subject><subject>Microvilli</subject><subject>Morphogenesis</subject><subject>Myosins - analysis</subject><subject>Phosphoproteins - analysis</subject><subject>Recombinant Fusion Proteins</subject><subject>RNA, Messenger - analysis</subject><subject>small intestine</subject><subject>Sucrase-Isomaltase Complex - analysis</subject><subject>Zonula Occludens-1 Protein</subject><subject>β‐galactosidase</subject><issn>1058-8388</issn><issn>1097-0177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EKqXwE5B8Qu0hiyfxxvZSVVqlfCyqtAcKXJBG3mTSGuULOwvqv6_TrHoBiZPtd-Z9Z_wwdgFiAUKkb0-_bIrNGQijEgFKnYIxWsBZCrCC8yivVuvNZbL-vAZxkS3Eoti-SxP1hB0_Wp5O96VOdKb1c_YihJ9CCJ1LOGJHRmZapvqY_bi2_oZGqnjlgt8Po-s73td8vCXe9vtA_LdrGtfxG-qIVz0F3vUjd-1gnT90-eG2n8rBhcnautL3D66X7Fltm0CvDucJ-_rh_XXxKbnaftwU66uklDITiaokkDWmVCXVtdRLUQEIbXYp1blKla3iC-qdMJKyypYRBVlapqRLCbqW2Ql7M-cOvv-1pzBi60JJTWM7in9AZfIccpXHxm9zY9wwBE81Dt611t8hCJywI07YcWKIE0OcsWPEjjDJiBE7PmDHDAUWW0xRxeDXhw32u5aqx9gD51j_Ptf_uIbu_pr6v6H_mjkL2T2QB54j</recordid><startdate>199801</startdate><enddate>199801</enddate><creator>Pinson, Kathleen I.</creator><creator>Dunbar, Laura</creator><creator>Samuelson, Linda</creator><creator>Gumucio, Deborah L.</creator><general>Wiley‐Liss, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199801</creationdate><title>Targeted disruption of the mouse villin gene does not impair the morphogenesis of microvilli</title><author>Pinson, Kathleen I. ; Dunbar, Laura ; Samuelson, Linda ; Gumucio, Deborah L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4430-7d41ea99c7ceff4850d11089b2ef6727ad1081fb094e3dac199eae52e8c418f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>brush border development</topic><topic>Carrier Proteins - analysis</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - physiology</topic><topic>embryonic stem cells</topic><topic>Epithelial Cells - ultrastructure</topic><topic>Female</topic><topic>Gene Targeting</topic><topic>Genes - physiology</topic><topic>homologous recombination</topic><topic>Intestine, Small - chemistry</topic><topic>Intestine, Small - embryology</topic><topic>Intestine, Small - ultrastructure</topic><topic>Male</topic><topic>Membrane Proteins - analysis</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Microfilament Proteins - analysis</topic><topic>Microfilament Proteins - genetics</topic><topic>Microfilament Proteins - physiology</topic><topic>Microvilli</topic><topic>Morphogenesis</topic><topic>Myosins - analysis</topic><topic>Phosphoproteins - analysis</topic><topic>Recombinant Fusion Proteins</topic><topic>RNA, Messenger - analysis</topic><topic>small intestine</topic><topic>Sucrase-Isomaltase Complex - analysis</topic><topic>Zonula Occludens-1 Protein</topic><topic>β‐galactosidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pinson, Kathleen I.</creatorcontrib><creatorcontrib>Dunbar, Laura</creatorcontrib><creatorcontrib>Samuelson, Linda</creatorcontrib><creatorcontrib>Gumucio, Deborah L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental dynamics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pinson, Kathleen I.</au><au>Dunbar, Laura</au><au>Samuelson, Linda</au><au>Gumucio, Deborah L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Targeted disruption of the mouse villin gene does not impair the morphogenesis of microvilli</atitle><jtitle>Developmental dynamics</jtitle><addtitle>Dev Dyn</addtitle><date>1998-01</date><risdate>1998</risdate><volume>211</volume><issue>1</issue><spage>109</spage><epage>121</epage><pages>109-121</pages><issn>1058-8388</issn><eissn>1097-0177</eissn><abstract>The small intestine is functionally dependent on the presence of the brush border, a tightly packed array of microvilli that forms the amplified apical surface of absorptive cells. 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subjects Animals
brush border development
Carrier Proteins - analysis
Carrier Proteins - genetics
Carrier Proteins - physiology
embryonic stem cells
Epithelial Cells - ultrastructure
Female
Gene Targeting
Genes - physiology
homologous recombination
Intestine, Small - chemistry
Intestine, Small - embryology
Intestine, Small - ultrastructure
Male
Membrane Proteins - analysis
Mice
Mice, Knockout
Microfilament Proteins - analysis
Microfilament Proteins - genetics
Microfilament Proteins - physiology
Microvilli
Morphogenesis
Myosins - analysis
Phosphoproteins - analysis
Recombinant Fusion Proteins
RNA, Messenger - analysis
small intestine
Sucrase-Isomaltase Complex - analysis
Zonula Occludens-1 Protein
β‐galactosidase
title Targeted disruption of the mouse villin gene does not impair the morphogenesis of microvilli
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