Polyadenylated RNA during DAPI-arrested regeneration of Tetrahymena cilia
Regeneration of cilia in Tetrahymena cells treated with 1 micrograms/ml of 4',6-diamidino-2-phenylindole (DAPI) takes place about 30 min later then in untreated controls. Analysis of RNA isolated at 50-th min of reciliation, that is when the control cells-but not DAPI-treated ones-have just res...
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| Veröffentlicht in: | Molecular and cellular biochemistry 1990-01, Vol.92 (1), p.53-60 |
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| container_title | Molecular and cellular biochemistry |
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| creator | Krawczyńska, W Kludkiewicz, B |
| description | Regeneration of cilia in Tetrahymena cells treated with 1 micrograms/ml of 4',6-diamidino-2-phenylindole (DAPI) takes place about 30 min later then in untreated controls. Analysis of RNA isolated at 50-th min of reciliation, that is when the control cells-but not DAPI-treated ones-have just restored their motility, revealed that; - 1. Two peaks of polyadenylated RNA were present in DAPI-treated cells as well as in the control ones; - 2. The mobility of the polyadenylated RNA was the same in the two compared cell samples; - 3. In wheat germ cell-free translational system these poly (A+)-RNA probably directed the synthesis of alpha- and beta-tubulin; - 4. The amount of polyadenylated RNA in compared cell samples was different, in DAPI-treated Tetrahymena its amount was estimated at 1.43% vs. 1.89% in control ones. |
| doi_str_mv | 10.1007/BF00220719 |
| format | Article |
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Analysis of RNA isolated at 50-th min of reciliation, that is when the control cells-but not DAPI-treated ones-have just restored their motility, revealed that; - 1. Two peaks of polyadenylated RNA were present in DAPI-treated cells as well as in the control ones; - 2. The mobility of the polyadenylated RNA was the same in the two compared cell samples; - 3. In wheat germ cell-free translational system these poly (A+)-RNA probably directed the synthesis of alpha- and beta-tubulin; - 4. 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Analysis of RNA isolated at 50-th min of reciliation, that is when the control cells-but not DAPI-treated ones-have just restored their motility, revealed that; - 1. Two peaks of polyadenylated RNA were present in DAPI-treated cells as well as in the control ones; - 2. The mobility of the polyadenylated RNA was the same in the two compared cell samples; - 3. In wheat germ cell-free translational system these poly (A+)-RNA probably directed the synthesis of alpha- and beta-tubulin; - 4. The amount of polyadenylated RNA in compared cell samples was different, in DAPI-treated Tetrahymena its amount was estimated at 1.43% vs. 1.89% in control ones.</description><subject>Animals</subject><subject>Cilia - drug effects</subject><subject>Cilia - physiology</subject><subject>Electrophoresis</subject><subject>Indoles - pharmacology</subject><subject>Nucleic Acid Denaturation</subject><subject>Poly A - analysis</subject><subject>Protein Biosynthesis</subject><subject>Regeneration - drug effects</subject><subject>RNA - analysis</subject><subject>Tetrahymena pyriformis - drug effects</subject><subject>Tetrahymena pyriformis - genetics</subject><subject>Tubulin - biosynthesis</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLw0AUhQdRaq1u3AtZuRCidyaZ17JWq4WiReo6TDN3aiSPOpMs8u9NaaGrszgfh8NHyC2FRwogn57nAIyBpPqMjCmXSZxqqs_JGBKAWFEpL8lVCL8AA07piIyoUFqBGJPFqil7Y7HuS9Oijb4-ppHtfFFvo5fpahEb7zHsC49brNGbtmjqqHHRGltvfvoKaxPlRVmYa3LhTBnw5pgT8j1_Xc_e4-Xn22I2XcY5S2kbp447Bk45UCJJNac550xvkAFPhaCOgjJgtOZcOmM1co15whxoZ5XjYJMJuT_s7nzz1w3nsqoIOZalqbHpQia1EMCFGMCHA5j7JgSPLtv5ojK-zyhke2_ZydsA3x1Xu02F9oQeRCX_V7tmYg</recordid><startdate>19900118</startdate><enddate>19900118</enddate><creator>Krawczyńska, W</creator><creator>Kludkiewicz, B</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900118</creationdate><title>Polyadenylated RNA during DAPI-arrested regeneration of Tetrahymena cilia</title><author>Krawczyńska, W ; Kludkiewicz, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c241t-4f5f20f8f08634951c5529be2054661f108a0a99557fad9e59ec32f09fd8f50d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Cilia - drug effects</topic><topic>Cilia - physiology</topic><topic>Electrophoresis</topic><topic>Indoles - pharmacology</topic><topic>Nucleic Acid Denaturation</topic><topic>Poly A - analysis</topic><topic>Protein Biosynthesis</topic><topic>Regeneration - drug effects</topic><topic>RNA - analysis</topic><topic>Tetrahymena pyriformis - drug effects</topic><topic>Tetrahymena pyriformis - genetics</topic><topic>Tubulin - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krawczyńska, W</creatorcontrib><creatorcontrib>Kludkiewicz, B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krawczyńska, W</au><au>Kludkiewicz, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polyadenylated RNA during DAPI-arrested regeneration of Tetrahymena cilia</atitle><jtitle>Molecular and cellular biochemistry</jtitle><addtitle>Mol Cell Biochem</addtitle><date>1990-01-18</date><risdate>1990</risdate><volume>92</volume><issue>1</issue><spage>53</spage><epage>60</epage><pages>53-60</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>Regeneration of cilia in Tetrahymena cells treated with 1 micrograms/ml of 4',6-diamidino-2-phenylindole (DAPI) takes place about 30 min later then in untreated controls. Analysis of RNA isolated at 50-th min of reciliation, that is when the control cells-but not DAPI-treated ones-have just restored their motility, revealed that; - 1. Two peaks of polyadenylated RNA were present in DAPI-treated cells as well as in the control ones; - 2. The mobility of the polyadenylated RNA was the same in the two compared cell samples; - 3. In wheat germ cell-free translational system these poly (A+)-RNA probably directed the synthesis of alpha- and beta-tubulin; - 4. The amount of polyadenylated RNA in compared cell samples was different, in DAPI-treated Tetrahymena its amount was estimated at 1.43% vs. 1.89% in control ones.</abstract><cop>Netherlands</cop><pmid>1689806</pmid><doi>10.1007/BF00220719</doi><tpages>8</tpages></addata></record> |
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| ispartof | Molecular and cellular biochemistry, 1990-01, Vol.92 (1), p.53-60 |
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| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Cilia - drug effects Cilia - physiology Electrophoresis Indoles - pharmacology Nucleic Acid Denaturation Poly A - analysis Protein Biosynthesis Regeneration - drug effects RNA - analysis Tetrahymena pyriformis - drug effects Tetrahymena pyriformis - genetics Tubulin - biosynthesis |
| title | Polyadenylated RNA during DAPI-arrested regeneration of Tetrahymena cilia |
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