The origin of DNA associated with mucus glycoproteins in cystic fibrosis sputum
The relative importance of host and bacteria-derived deoxyribonucleic acid (DNA) in the increased viscoelasticity of purulent sputum in cystic fibrosis (CF) and other airway diseases is unclear. We report the identification of the DNA associated with mucus glycoproteins purified from the purulent sp...
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| Veröffentlicht in: | The European respiratory journal 1990-01, Vol.3 (1), p.19-23 |
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| creator | Lethem, MI James, SL Marriott, C Burke, JF |
| description | The relative importance of host and bacteria-derived deoxyribonucleic acid (DNA) in the increased viscoelasticity of purulent sputum in cystic fibrosis (CF) and other airway diseases is unclear. We report the identification of the DNA associated with mucus glycoproteins purified from the purulent sputum of 9 patients with CF. Mucus glycoproteins were purified from CF sputum by gel exclusion chromatography and the co-purifying DNA isolated by phenol extraction. Electrophoresis indicated that the DNA preparations had a size of approximately 300 to greater than 50,000 bases. The origin of the DNA was determined by slot blotting and subsequent hybridization with 32P-labelled DNA probes specific for human DNA sequences and those from bacterial species commonly isolated from CF sputum. The results indicated that in all cases the DNA was almost entirely human in origin. This implies that it is the patient's own DNA which may contribute to the rheological abnormalities of CF sputum. |
| doi_str_mv | 10.1183/09031936.93.03010019 |
| format | Article |
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We report the identification of the DNA associated with mucus glycoproteins purified from the purulent sputum of 9 patients with CF. Mucus glycoproteins were purified from CF sputum by gel exclusion chromatography and the co-purifying DNA isolated by phenol extraction. Electrophoresis indicated that the DNA preparations had a size of approximately 300 to greater than 50,000 bases. The origin of the DNA was determined by slot blotting and subsequent hybridization with 32P-labelled DNA probes specific for human DNA sequences and those from bacterial species commonly isolated from CF sputum. The results indicated that in all cases the DNA was almost entirely human in origin. This implies that it is the patient's own DNA which may contribute to the rheological abnormalities of CF sputum.</description><identifier>ISSN: 0903-1936</identifier><identifier>EISSN: 1399-3003</identifier><identifier>DOI: 10.1183/09031936.93.03010019</identifier><identifier>PMID: 2107097</identifier><language>eng</language><publisher>Leeds: Eur Respiratory Soc</publisher><subject>Adolescent ; Adult ; Biological and medical sciences ; Child ; Cystic Fibrosis - genetics ; DNA - analysis ; DNA - biosynthesis ; DNA, Bacterial - analysis ; DNA, Bacterial - biosynthesis ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Glycoproteins - analysis ; Humans ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Male ; Medical sciences ; Mucus - analysis ; Other diseases. Semiology ; Pseudomonas aeruginosa - metabolism ; Sputum - analysis</subject><ispartof>The European respiratory journal, 1990-01, Vol.3 (1), p.19-23</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-da29a4c4d4ab3cd3e5d9c459b7cd93a1f67f4f3814375792a89bbece7176a3d03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19417800$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2107097$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lethem, MI</creatorcontrib><creatorcontrib>James, SL</creatorcontrib><creatorcontrib>Marriott, C</creatorcontrib><creatorcontrib>Burke, JF</creatorcontrib><title>The origin of DNA associated with mucus glycoproteins in cystic fibrosis sputum</title><title>The European respiratory journal</title><addtitle>Eur Respir J</addtitle><description>The relative importance of host and bacteria-derived deoxyribonucleic acid (DNA) in the increased viscoelasticity of purulent sputum in cystic fibrosis (CF) and other airway diseases is unclear. We report the identification of the DNA associated with mucus glycoproteins purified from the purulent sputum of 9 patients with CF. Mucus glycoproteins were purified from CF sputum by gel exclusion chromatography and the co-purifying DNA isolated by phenol extraction. Electrophoresis indicated that the DNA preparations had a size of approximately 300 to greater than 50,000 bases. The origin of the DNA was determined by slot blotting and subsequent hybridization with 32P-labelled DNA probes specific for human DNA sequences and those from bacterial species commonly isolated from CF sputum. The results indicated that in all cases the DNA was almost entirely human in origin. This implies that it is the patient's own DNA which may contribute to the rheological abnormalities of CF sputum.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Cystic Fibrosis - genetics</subject><subject>DNA - analysis</subject><subject>DNA - biosynthesis</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - biosynthesis</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Glycoproteins - analysis</subject><subject>Humans</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mucus - analysis</subject><subject>Other diseases. Semiology</subject><subject>Pseudomonas aeruginosa - metabolism</subject><subject>Sputum - analysis</subject><issn>0903-1936</issn><issn>1399-3003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEFP4zAQhS20iO0C_wAkSyv2lmJ3nDhzRAUWJAQXOFuO47RGSVM8iVD_Pa6aZU8z0vve09Nj7EKKuZQlXAsUIBGKOcJcgJBCSDxiMwmIGQgBP9hsj2R75if7RfSeiEKBPGEnCym0QD1jL69rz_sYVmHD-4bfPt9wS9S7YAdf888wrHk3upH4qt25fhv7wYcN8US7HQ3B8SZUsadAnLbjMHZn7LixLfnz6Z6yt_u71-VD9vTy93F585Q5gHLIartAq5yqla3A1eDzGp3KsdKuRrCyKXSjGiilAp1rXNgSq8o7r6UuLNQCTtmfQ26q9DF6GkwXyPm2tRvfj2Q0FnmJ-SKB6gC6VJOib8w2hs7GnZHC7Hc0_3Y0mP5px2S7nPLHqvP1t2kaLulXk27J2baJduMC_c9GJXUp9j1_H7h1WK0_Q_SGOtu2KVUaH9_ByMTCF9Dqh30</recordid><startdate>19900101</startdate><enddate>19900101</enddate><creator>Lethem, MI</creator><creator>James, SL</creator><creator>Marriott, C</creator><creator>Burke, JF</creator><general>Eur Respiratory Soc</general><general>Maney</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900101</creationdate><title>The origin of DNA associated with mucus glycoproteins in cystic fibrosis sputum</title><author>Lethem, MI ; James, SL ; Marriott, C ; Burke, JF</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-da29a4c4d4ab3cd3e5d9c459b7cd93a1f67f4f3814375792a89bbece7176a3d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Cystic Fibrosis - genetics</topic><topic>DNA - analysis</topic><topic>DNA - biosynthesis</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - biosynthesis</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Glycoproteins - analysis</topic><topic>Humans</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mucus - analysis</topic><topic>Other diseases. Semiology</topic><topic>Pseudomonas aeruginosa - metabolism</topic><topic>Sputum - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lethem, MI</creatorcontrib><creatorcontrib>James, SL</creatorcontrib><creatorcontrib>Marriott, C</creatorcontrib><creatorcontrib>Burke, JF</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The European respiratory journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lethem, MI</au><au>James, SL</au><au>Marriott, C</au><au>Burke, JF</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The origin of DNA associated with mucus glycoproteins in cystic fibrosis sputum</atitle><jtitle>The European respiratory journal</jtitle><addtitle>Eur Respir J</addtitle><date>1990-01-01</date><risdate>1990</risdate><volume>3</volume><issue>1</issue><spage>19</spage><epage>23</epage><pages>19-23</pages><issn>0903-1936</issn><eissn>1399-3003</eissn><abstract>The relative importance of host and bacteria-derived deoxyribonucleic acid (DNA) in the increased viscoelasticity of purulent sputum in cystic fibrosis (CF) and other airway diseases is unclear. We report the identification of the DNA associated with mucus glycoproteins purified from the purulent sputum of 9 patients with CF. Mucus glycoproteins were purified from CF sputum by gel exclusion chromatography and the co-purifying DNA isolated by phenol extraction. Electrophoresis indicated that the DNA preparations had a size of approximately 300 to greater than 50,000 bases. The origin of the DNA was determined by slot blotting and subsequent hybridization with 32P-labelled DNA probes specific for human DNA sequences and those from bacterial species commonly isolated from CF sputum. The results indicated that in all cases the DNA was almost entirely human in origin. This implies that it is the patient's own DNA which may contribute to the rheological abnormalities of CF sputum.</abstract><cop>Leeds</cop><pub>Eur Respiratory Soc</pub><pmid>2107097</pmid><doi>10.1183/09031936.93.03010019</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The European respiratory journal, 1990-01, Vol.3 (1), p.19-23 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adolescent Adult Biological and medical sciences Child Cystic Fibrosis - genetics DNA - analysis DNA - biosynthesis DNA, Bacterial - analysis DNA, Bacterial - biosynthesis Female Gastroenterology. Liver. Pancreas. Abdomen Glycoproteins - analysis Humans Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences Mucus - analysis Other diseases. Semiology Pseudomonas aeruginosa - metabolism Sputum - analysis |
| title | The origin of DNA associated with mucus glycoproteins in cystic fibrosis sputum |
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