Colorimetric Glucose Assay Using Thermostable Glucokinase

A method for assaying glucose in serum or plasma samples using a thermostable glucokinase was developed. Glucokinase from Bacillus Stearothermophilus was coupled with glucose-6-phosphate dehydrogenase to produce NADPH, which reduced the tetrazolium dye MTT to its formazan. Detection of the product a...

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Veröffentlicht in:	Annals of clinical biochemistry 1990-01, Vol.27 (1), p.33-37
Hauptverfasser:	Scott, D A, Goward, C R, Scawen, M D, Atkinson, T, Price, C P
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Blood Glucose - analysis Colorimetry Dithiothreitol Enzyme Stability Formazans Geobacillus stearothermophilus - enzymology Glucokinase Hexokinase Hot Temperature Investigative techniques, diagnostic techniques (general aspects) Kinetics Medical sciences Miscellaneous. Technology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Spectrophotometry, Ultraviolet Tetrazolium Salts Thiazoles
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