Investigation of the Binding of Isoform-Selective Inhibitors to Prostaglandin Endoperoxide Synthases Using Fluorescence Spectroscopy

Prostaglandin endoperoxide synthase (PGHS) is a heme protein that catalyzes the committed step in prostaglandin and thromboxane biosynthesis. Two isoforms of PGHS exist, a constitutive form termed PGHS-1 and an inducible form termed PGHS-2. We report here fluorescence resonance energy transfer analy...

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Veröffentlicht in:	Biochemistry (Easton) 1998-01, Vol.37 (1), p.217-226
Hauptverfasser:	Lanzo, Cheryl A, Beechem, Joseph M, Talley, John, Marnett, Lawrence J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoenzymes - metabolism Binding, Competitive Cyclooxygenase 1 Cyclooxygenase 2 Cyclooxygenase 2 Inhibitors Cyclooxygenase Inhibitors - metabolism Energy Transfer Fluorescence Polarization Flurbiprofen - metabolism Heme - metabolism Humans Isoenzymes - metabolism Kinetics Membrane Proteins Models, Molecular Prostaglandin-Endoperoxide Synthases - metabolism Protein Binding - drug effects Quantum Theory Sheep Spectrometry, Fluorescence Thiazoles - metabolism Thiazoles - pharmacology Titrimetry
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