Dioxygen Inactivation of Pyruvate Formate-Lyase:  EPR Evidence for the Formation of Protein-Based Sulfinyl and Peroxyl Radicals

We here report EPR studies that provide evidence for radical intermediates generated from the glycyl radical of activated pyruvate formate-lyase (PFL) during the process of oxygen-dependent enzyme inactivation, radical quenching, and protein fragmentation. Upon exposure of active PFL to air, a long-...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Biochemistry (Easton) 1998-01, Vol.37 (2), p.558-563
Hauptverfasser:	Reddy, Sreelatha G, Wong, Kenny K, Parast, Camran V, Peisach, Jack, Magliozzo, Richard S, Kozarich, John W
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetyltransferases - drug effects Cysteine - metabolism Electron Spin Resonance Spectroscopy Escherichia coli - enzymology Free Radicals - metabolism Glycine - metabolism Models, Chemical Oxygen - pharmacology Peptide Fragments - metabolism Peroxides - metabolism Sulfinic Acids - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!