Studies on subtype composition in natural leukocyte interferon preparations

Antisera raised against partially purified human leukocyte interferon in goat and horse were exhaustively adsorbed and purified. Affinity chromatography using these antibodies gave 10000-fold purification in one step without losing interferon activity. Then enzyme immunoassay was established with th...

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Veröffentlicht in:	Journal of virological methods 1990, Vol.27 (1), p.1-9
Hauptverfasser:	Shirono, Hiroyuki, Ito, Chitose, Koga, Junichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis of the immune response. Humoral and cellular immunity Animals Antibodies - immunology Biological and medical sciences Blotting, Western Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel Enzyme immunoassay Fundamental and applied biological sciences. Psychology Fundamental immunology Human leukocyte interferon Humans Immunobiology Immunoenzyme Techniques Interferon Type I Leukocytes - analysis Leukocytes - immunology Microbiology Miscellaneous Regulatory factors and their cellular receptors Reverse phase high performance liquid chromatography Subtype composition
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