Thyroid peroxidase gene promoter confers TSH responsiveness to heterologous reporter genes in transfection experiments
The cyclic AMP-mediated transcriptional regulation of the enzyme thyroperoxidase by thyrotropin (TSH) in thyroid follicular cells was examined at the molecular level. The 5′ end of the human thyroperoxidase gene was isolated and sequenced and the transcription start site was mapped by S1 nuclease an...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1990-02, Vol.166 (3), p.1257-1264 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Abramowicz, Marc J. Vassart, Gilbert Christophe, Daniel |
| description | The cyclic AMP-mediated transcriptional regulation of the enzyme thyroperoxidase by thyrotropin (TSH) in thyroid follicular cells was examined at the molecular level. The 5′ end of the human thyroperoxidase gene was isolated and sequenced and the transcription start site was mapped by S1 nuclease analysis. A 0.9 kilobase pair DNA fragment of the promoter was shown to confer responsiveness to thyrotropin, and cyclic AMP, in transient expression assays using two different reporter genes. Several potential sites for specific interaction with nuclear transcription factors which could be involved in the regulation of thyroperoxidase gene transcription were identified. |
| doi_str_mv | 10.1016/0006-291X(90)91001-9 |
| format | Article |
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The 5′ end of the human thyroperoxidase gene was isolated and sequenced and the transcription start site was mapped by S1 nuclease analysis. A 0.9 kilobase pair DNA fragment of the promoter was shown to confer responsiveness to thyrotropin, and cyclic AMP, in transient expression assays using two different reporter genes. 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Psychology ; Genes - drug effects ; Genome, Human ; Humans ; Iodide Peroxidase - genetics ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Oligonucleotide Probes ; Promoter Regions, Genetic - drug effects ; Restriction Mapping ; Thyroid Gland - enzymology ; Thyrotropin - pharmacology ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. 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The 5′ end of the human thyroperoxidase gene was isolated and sequenced and the transcription start site was mapped by S1 nuclease analysis. A 0.9 kilobase pair DNA fragment of the promoter was shown to confer responsiveness to thyrotropin, and cyclic AMP, in transient expression assays using two different reporter genes. Several potential sites for specific interaction with nuclear transcription factors which could be involved in the regulation of thyroperoxidase gene transcription were identified.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Chloramphenicol O-Acetyltransferase - genetics</subject><subject>Cloning, Molecular</subject><subject>Colforsin - pharmacology</subject><subject>DNA - genetics</subject><subject>Dogs</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes - drug effects</subject><subject>Genome, Human</subject><subject>Humans</subject><subject>Iodide Peroxidase - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Oligonucleotide Probes</subject><subject>Promoter Regions, Genetic - drug effects</subject><subject>Restriction Mapping</subject><subject>Thyroid Gland - enzymology</subject><subject>Thyrotropin - pharmacology</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. 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Psychology</topic><topic>Genes - drug effects</topic><topic>Genome, Human</topic><topic>Humans</topic><topic>Iodide Peroxidase - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Oligonucleotide Probes</topic><topic>Promoter Regions, Genetic - drug effects</topic><topic>Restriction Mapping</topic><topic>Thyroid Gland - enzymology</topic><topic>Thyrotropin - pharmacology</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abramowicz, Marc J.</creatorcontrib><creatorcontrib>Vassart, Gilbert</creatorcontrib><creatorcontrib>Christophe, Daniel</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abramowicz, Marc J.</au><au>Vassart, Gilbert</au><au>Christophe, Daniel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thyroid peroxidase gene promoter confers TSH responsiveness to heterologous reporter genes in transfection experiments</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1990-02-14</date><risdate>1990</risdate><volume>166</volume><issue>3</issue><spage>1257</spage><epage>1264</epage><pages>1257-1264</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>The cyclic AMP-mediated transcriptional regulation of the enzyme thyroperoxidase by thyrotropin (TSH) in thyroid follicular cells was examined at the molecular level. The 5′ end of the human thyroperoxidase gene was isolated and sequenced and the transcription start site was mapped by S1 nuclease analysis. A 0.9 kilobase pair DNA fragment of the promoter was shown to confer responsiveness to thyrotropin, and cyclic AMP, in transient expression assays using two different reporter genes. Several potential sites for specific interaction with nuclear transcription factors which could be involved in the regulation of thyroperoxidase gene transcription were identified.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2306241</pmid><doi>10.1016/0006-291X(90)91001-9</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1990-02, Vol.166 (3), p.1257-1264 |
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| subjects | Animals Base Sequence Biological and medical sciences Cells, Cultured Chloramphenicol O-Acetyltransferase - genetics Cloning, Molecular Colforsin - pharmacology DNA - genetics Dogs Fundamental and applied biological sciences. Psychology Genes - drug effects Genome, Human Humans Iodide Peroxidase - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Oligonucleotide Probes Promoter Regions, Genetic - drug effects Restriction Mapping Thyroid Gland - enzymology Thyrotropin - pharmacology Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing Transfection |
| title | Thyroid peroxidase gene promoter confers TSH responsiveness to heterologous reporter genes in transfection experiments |
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