Two Versions of the Gene Encoding the 41-Kilodalton Subunit of the Telomere Binding Protein of Oxytricha Nova
Macronuclear chromosomes of the ciliated protozoan Oxytricha nova terminate with a single-stranded (T4G 4)2overhang. The (T4G 4)2telomeric overhang is tenaciously bound by a protein heterodimer. We have cloned and sequenced the gene encoding the 41-kDa subunit of this telomere binding protein. The p...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1990-02, Vol.87 (4), p.1481-1485 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Hicke, Brian J. Celander, Daniel W. MacDonald, Gregg H. Price, Carolyn M. Cech, Thomas R. |
| description | Macronuclear chromosomes of the ciliated protozoan Oxytricha nova terminate with a single-stranded (T4G
4)2overhang. The (T4G
4)2telomeric overhang is tenaciously bound by a protein heterodimer. We have cloned and sequenced the gene encoding the 41-kDa subunit of this telomere binding protein. The predicted amino acid sequence comprises two distinct regions, a carboxyl-terminal two-thirds that is 23% lysine and bears similarity to histone H1 and an amino-terminal one-third containing a hydrophobic stretch of about 15 amino acids. Two macronuclear versions of the gene differ in nucleotide sequence at several positions, but the derived polypeptides differ only at a single position, Ser-110 or Ala-110. Both versions harbor a small intron. The existence of this intron demonstrates that, despite the elimination of 95% of the micronuclear genome from the developing macronucleus, at least some noncoding DNA is retained during macronuclear development of hypotrichous ciliates. |
| doi_str_mv | 10.1073/pnas.87.4.1481 |
| format | Article |
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4)2overhang. The (T4G
4)2telomeric overhang is tenaciously bound by a protein heterodimer. We have cloned and sequenced the gene encoding the 41-kDa subunit of this telomere binding protein. The predicted amino acid sequence comprises two distinct regions, a carboxyl-terminal two-thirds that is 23% lysine and bears similarity to histone H1 and an amino-terminal one-third containing a hydrophobic stretch of about 15 amino acids. Two macronuclear versions of the gene differ in nucleotide sequence at several positions, but the derived polypeptides differ only at a single position, Ser-110 or Ala-110. Both versions harbor a small intron. The existence of this intron demonstrates that, despite the elimination of 95% of the micronuclear genome from the developing macronucleus, at least some noncoding DNA is retained during macronuclear development of hypotrichous ciliates.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.87.4.1481</identifier><identifier>PMID: 1689486</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Amino Acid Sequence ; Amino acids ; Animals ; Base Sequence ; Ciliophora - genetics ; Cloning, Molecular ; Codons ; DNA ; DNA - genetics ; DNA - isolation & purification ; DNA-binding protein ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - isolation & purification ; Gene Amplification ; Gene Library ; Genes ; Histones ; Information Systems ; Introns ; Molecular Sequence Data ; Nucleotide sequences ; Nucleotides ; Oligonucleotide Probes ; Peptide Mapping ; Protein Conformation ; Proteins ; RNA ; RNA - genetics ; RNA - isolation & purification ; Sequence Homology, Nucleic Acid ; Telomeres</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1990-02, Vol.87 (4), p.1481-1485</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4031-ed890f3d4a194a1954f46fd30d4f01163cac20eed7f54fae34d98c2c04d781353</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/87/4.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2353859$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2353859$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1689486$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hicke, Brian J.</creatorcontrib><creatorcontrib>Celander, Daniel W.</creatorcontrib><creatorcontrib>MacDonald, Gregg H.</creatorcontrib><creatorcontrib>Price, Carolyn M.</creatorcontrib><creatorcontrib>Cech, Thomas R.</creatorcontrib><title>Two Versions of the Gene Encoding the 41-Kilodalton Subunit of the Telomere Binding Protein of Oxytricha Nova</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Macronuclear chromosomes of the ciliated protozoan Oxytricha nova terminate with a single-stranded (T4G
4)2overhang. The (T4G
4)2telomeric overhang is tenaciously bound by a protein heterodimer. We have cloned and sequenced the gene encoding the 41-kDa subunit of this telomere binding protein. The predicted amino acid sequence comprises two distinct regions, a carboxyl-terminal two-thirds that is 23% lysine and bears similarity to histone H1 and an amino-terminal one-third containing a hydrophobic stretch of about 15 amino acids. Two macronuclear versions of the gene differ in nucleotide sequence at several positions, but the derived polypeptides differ only at a single position, Ser-110 or Ala-110. Both versions harbor a small intron. The existence of this intron demonstrates that, despite the elimination of 95% of the micronuclear genome from the developing macronucleus, at least some noncoding DNA is retained during macronuclear development of hypotrichous ciliates.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Ciliophora - genetics</subject><subject>Cloning, Molecular</subject><subject>Codons</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>DNA-binding protein</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - isolation & purification</subject><subject>Gene Amplification</subject><subject>Gene Library</subject><subject>Genes</subject><subject>Histones</subject><subject>Information Systems</subject><subject>Introns</subject><subject>Molecular Sequence Data</subject><subject>Nucleotide sequences</subject><subject>Nucleotides</subject><subject>Oligonucleotide Probes</subject><subject>Peptide Mapping</subject><subject>Protein Conformation</subject><subject>Proteins</subject><subject>RNA</subject><subject>RNA - genetics</subject><subject>RNA - isolation & purification</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Telomeres</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1vFCEYh4mxqWvr1ZMmc-ptRhiYGUi8aNNWY2NNunolFN7p0jCwAtOP_96Zbj_Wix4ICc_zI7z8EHpLcEVwRz-svUoV7ypWEcbJC7QgWJCyZQK_RAuM667krGav0OuUrjDGouF4F-2SlgvG2wUaljeh-AUx2eBTEfoir6A4AQ_FkdfBWH95f8JI-c26YJTLwRfn48XobX7Ul-DCABGKz9bfJ37EkMH6mZ_d3uVo9UoV38O12kc7vXIJ3jzse-jn8dHy8Et5enby9fDTaakZpqQEwwXuqWGKiHk1rGdtbyg2rMeEtFQrXWMA0_UTUkCZEVzXGjPTcUIbuoc-bu5djxcDGA0-R-XkOtpBxTsZlJV_E29X8jJcy4YyIab4wUM8ht8jpCwHmzQ4pzyEMclOtDWnzf9F0rAWC1FPYrURdQwpReif3kKwnHuUc4-Sd5LJuccp8H57gmd9U9wWn3OPdDt_8C8u-9G5DLd5Et9txKuUQ3wy6-kX-TThHyv1u4c</recordid><startdate>19900201</startdate><enddate>19900201</enddate><creator>Hicke, Brian J.</creator><creator>Celander, Daniel W.</creator><creator>MacDonald, Gregg H.</creator><creator>Price, Carolyn M.</creator><creator>Cech, Thomas R.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19900201</creationdate><title>Two Versions of the Gene Encoding the 41-Kilodalton Subunit of the Telomere Binding Protein of Oxytricha Nova</title><author>Hicke, Brian J. ; Celander, Daniel W. ; MacDonald, Gregg H. ; Price, Carolyn M. ; Cech, Thomas R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4031-ed890f3d4a194a1954f46fd30d4f01163cac20eed7f54fae34d98c2c04d781353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Ciliophora - genetics</topic><topic>Cloning, Molecular</topic><topic>Codons</topic><topic>DNA</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>DNA-binding protein</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - isolation & purification</topic><topic>Gene Amplification</topic><topic>Gene Library</topic><topic>Genes</topic><topic>Histones</topic><topic>Information Systems</topic><topic>Introns</topic><topic>Molecular Sequence Data</topic><topic>Nucleotide sequences</topic><topic>Nucleotides</topic><topic>Oligonucleotide Probes</topic><topic>Peptide Mapping</topic><topic>Protein Conformation</topic><topic>Proteins</topic><topic>RNA</topic><topic>RNA - genetics</topic><topic>RNA - isolation & purification</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Telomeres</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hicke, Brian J.</creatorcontrib><creatorcontrib>Celander, Daniel W.</creatorcontrib><creatorcontrib>MacDonald, Gregg H.</creatorcontrib><creatorcontrib>Price, Carolyn M.</creatorcontrib><creatorcontrib>Cech, Thomas R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hicke, Brian J.</au><au>Celander, Daniel W.</au><au>MacDonald, Gregg H.</au><au>Price, Carolyn M.</au><au>Cech, Thomas R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two Versions of the Gene Encoding the 41-Kilodalton Subunit of the Telomere Binding Protein of Oxytricha Nova</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1990-02-01</date><risdate>1990</risdate><volume>87</volume><issue>4</issue><spage>1481</spage><epage>1485</epage><pages>1481-1485</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Macronuclear chromosomes of the ciliated protozoan Oxytricha nova terminate with a single-stranded (T4G
4)2overhang. The (T4G
4)2telomeric overhang is tenaciously bound by a protein heterodimer. We have cloned and sequenced the gene encoding the 41-kDa subunit of this telomere binding protein. The predicted amino acid sequence comprises two distinct regions, a carboxyl-terminal two-thirds that is 23% lysine and bears similarity to histone H1 and an amino-terminal one-third containing a hydrophobic stretch of about 15 amino acids. Two macronuclear versions of the gene differ in nucleotide sequence at several positions, but the derived polypeptides differ only at a single position, Ser-110 or Ala-110. Both versions harbor a small intron. The existence of this intron demonstrates that, despite the elimination of 95% of the micronuclear genome from the developing macronucleus, at least some noncoding DNA is retained during macronuclear development of hypotrichous ciliates.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1689486</pmid><doi>10.1073/pnas.87.4.1481</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Amino acids Animals Base Sequence Ciliophora - genetics Cloning, Molecular Codons DNA DNA - genetics DNA - isolation & purification DNA-binding protein DNA-Binding Proteins - genetics DNA-Binding Proteins - isolation & purification Gene Amplification Gene Library Genes Histones Information Systems Introns Molecular Sequence Data Nucleotide sequences Nucleotides Oligonucleotide Probes Peptide Mapping Protein Conformation Proteins RNA RNA - genetics RNA - isolation & purification Sequence Homology, Nucleic Acid Telomeres |
| title | Two Versions of the Gene Encoding the 41-Kilodalton Subunit of the Telomere Binding Protein of Oxytricha Nova |
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