Drosophila GABAergic Systems: Sequence and Expression of Glutamic Acid Decarboxylase
: A mammalian glutamic acid decarboxylase (GAD) cDNA probe has been utilized to isolate Drosophila cDNA clones that represent a genomic locus in chromosome region 64A. Deletion analysis indicates that this chromosomal locus encodes an enzymatically active GAD protein. The in vitro translation of cRN...
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| Veröffentlicht in: | Journal of neurochemistry 1990-03, Vol.54 (3), p.1068-1078 |
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| container_title | Journal of neurochemistry |
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| creator | Jackson, F. Rob Newby, Laurel M. Kulkarni, Shankar J. |
| description | : A mammalian glutamic acid decarboxylase (GAD) cDNA probe has been utilized to isolate Drosophila cDNA clones that represent a genomic locus in chromosome region 64A. Deletion analysis indicates that this chromosomal locus encodes an enzymatically active GAD protein. The in vitro translation of cRNA representing a Drosophila cDNA clone yields a 57‐kDa protein that can be immunoprecipitated by an anti‐GAD antiserum. A GAD‐immunoreactive protein of the same size can also be detected in Drosophila head extracts. The nucleotide sequence derived from two overlapping Drosophila cDNA clones predicts a 57,759‐dalton protein composed of 510 residues that is 53% identical to mammalian GAD. Sequence comparisons of mammalian and Drosophila GAD identify two highly conserved regions (≥70% identity), one of which encompasses a putative cofactor‐binding domain. Transcriptional analyses show that expression of the Drosophila Gad gene commences early in embryonic development (4–8 h) and continues in all later developmental stages. A 3.1‐kb class of mRNA is detected throughout embryogenesis, in all three larval stages, in pupae, and in adults. This transcript class has a widespread distribution in the adult CNS. A smaller 2.6‐kb transcript is expressed in a developmentally regulated manner; it is detected only in embryos and pupae. |
| doi_str_mv | 10.1111/j.1471-4159.1990.tb02359.x |
| format | Article |
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Rob ; Newby, Laurel M. ; Kulkarni, Shankar J.</creator><creatorcontrib>Jackson, F. Rob ; Newby, Laurel M. ; Kulkarni, Shankar J.</creatorcontrib><description>: A mammalian glutamic acid decarboxylase (GAD) cDNA probe has been utilized to isolate Drosophila cDNA clones that represent a genomic locus in chromosome region 64A. Deletion analysis indicates that this chromosomal locus encodes an enzymatically active GAD protein. The in vitro translation of cRNA representing a Drosophila cDNA clone yields a 57‐kDa protein that can be immunoprecipitated by an anti‐GAD antiserum. A GAD‐immunoreactive protein of the same size can also be detected in Drosophila head extracts. The nucleotide sequence derived from two overlapping Drosophila cDNA clones predicts a 57,759‐dalton protein composed of 510 residues that is 53% identical to mammalian GAD. Sequence comparisons of mammalian and Drosophila GAD identify two highly conserved regions (≥70% identity), one of which encompasses a putative cofactor‐binding domain. Transcriptional analyses show that expression of the Drosophila Gad gene commences early in embryonic development (4–8 h) and continues in all later developmental stages. A 3.1‐kb class of mRNA is detected throughout embryogenesis, in all three larval stages, in pupae, and in adults. This transcript class has a widespread distribution in the adult CNS. A smaller 2.6‐kb transcript is expressed in a developmentally regulated manner; it is detected only in embryos and pupae.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.1990.tb02359.x</identifier><identifier>PMID: 1689376</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biochemistry. Physiology. Immunology ; Biological and medical sciences ; Central Nervous System - metabolism ; Chromosome Deletion ; Cloning, Molecular ; Diptera ; DNA - genetics ; DOPA decarboxylase ; Dopa Decarboxylase - genetics ; Drosophila melanogaster ; Drosophila melanogaster - genetics ; Drosophila melanogaster - physiology ; Drosophilidae ; Forecasting ; Fundamental and applied biological sciences. Psychology ; gamma-Aminobutyric Acid - physiology ; Gene ; Gene Expression Regulation, Enzymologic ; Glutamate Decarboxylase - genetics ; Glutamate Decarboxylase - metabolism ; Glutamic acid decarboxylase ; Immunologic Techniques ; inhibitory ; Insect ; Insecta ; Invertebrates ; Molecular Sequence Data ; Neurotransmitters ; Physiology. Development ; Protein Biosynthesis ; RNA - genetics ; RNA, Complementary ; Transcription, Genetic</subject><ispartof>Journal of neurochemistry, 1990-03, Vol.54 (3), p.1068-1078</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4308-87da131533e5e5949790ff2e882f8097475ca0037bbe34b365999acb8693be983</citedby><cites>FETCH-LOGICAL-c4308-87da131533e5e5949790ff2e882f8097475ca0037bbe34b365999acb8693be983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1471-4159.1990.tb02359.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1471-4159.1990.tb02359.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6817986$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1689376$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jackson, F. Rob</creatorcontrib><creatorcontrib>Newby, Laurel M.</creatorcontrib><creatorcontrib>Kulkarni, Shankar J.</creatorcontrib><title>Drosophila GABAergic Systems: Sequence and Expression of Glutamic Acid Decarboxylase</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: A mammalian glutamic acid decarboxylase (GAD) cDNA probe has been utilized to isolate Drosophila cDNA clones that represent a genomic locus in chromosome region 64A. Deletion analysis indicates that this chromosomal locus encodes an enzymatically active GAD protein. The in vitro translation of cRNA representing a Drosophila cDNA clone yields a 57‐kDa protein that can be immunoprecipitated by an anti‐GAD antiserum. A GAD‐immunoreactive protein of the same size can also be detected in Drosophila head extracts. The nucleotide sequence derived from two overlapping Drosophila cDNA clones predicts a 57,759‐dalton protein composed of 510 residues that is 53% identical to mammalian GAD. Sequence comparisons of mammalian and Drosophila GAD identify two highly conserved regions (≥70% identity), one of which encompasses a putative cofactor‐binding domain. Transcriptional analyses show that expression of the Drosophila Gad gene commences early in embryonic development (4–8 h) and continues in all later developmental stages. A 3.1‐kb class of mRNA is detected throughout embryogenesis, in all three larval stages, in pupae, and in adults. This transcript class has a widespread distribution in the adult CNS. A smaller 2.6‐kb transcript is expressed in a developmentally regulated manner; it is detected only in embryos and pupae.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biochemistry. Physiology. Immunology</subject><subject>Biological and medical sciences</subject><subject>Central Nervous System - metabolism</subject><subject>Chromosome Deletion</subject><subject>Cloning, Molecular</subject><subject>Diptera</subject><subject>DNA - genetics</subject><subject>DOPA decarboxylase</subject><subject>Dopa Decarboxylase - genetics</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila melanogaster - physiology</subject><subject>Drosophilidae</subject><subject>Forecasting</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma-Aminobutyric Acid - physiology</subject><subject>Gene</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Glutamate Decarboxylase - genetics</subject><subject>Glutamate Decarboxylase - metabolism</subject><subject>Glutamic acid decarboxylase</subject><subject>Immunologic Techniques</subject><subject>inhibitory</subject><subject>Insect</subject><subject>Insecta</subject><subject>Invertebrates</subject><subject>Molecular Sequence Data</subject><subject>Neurotransmitters</subject><subject>Physiology. Development</subject><subject>Protein Biosynthesis</subject><subject>RNA - genetics</subject><subject>RNA, Complementary</subject><subject>Transcription, Genetic</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkMtOAjEUhhujQUQfwWRijLvBdjrTixuDgKghugDXTaec0SFzwZaJ8PZ2AsGlsZuT5v_OJR9CVwT3iX-3yz6JOQljksg-kRL31ymOqP9sjlD3EB2jLsZRFFIcR6fozLklxoTFjHRQhzAhKWddNB_Z2tWrz7zQwWTwMAD7kZtgtnVrKN1dMIOvBioDga4WwXizsuBcXldBnQWTolnr0sMDky-CERht03qzLbSDc3SS6cLBxb720PvjeD58Cqdvk-fhYBqamGIRCr7QhJKEUkggkbHkEmdZBEJEmcCSxzwxGmPK0xRonFKWSCm1SQWTNAUpaA_d7OaubO3vdGtV5s5AUegK6sYpLlnEvYI_QZL4XZi2E-92oPFanIVMrWxeartVBKvWvVqqVrBqBavWvdq7VxvffLnf0qQlLH5bd7J9fr3PtTO6yKyuTO4OGBOES9Fi9zvsOy9g-48D1MvrkGAm6A8uUJ98</recordid><startdate>199003</startdate><enddate>199003</enddate><creator>Jackson, F. Rob</creator><creator>Newby, Laurel M.</creator><creator>Kulkarni, Shankar J.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199003</creationdate><title>Drosophila GABAergic Systems: Sequence and Expression of Glutamic Acid Decarboxylase</title><author>Jackson, F. Rob ; Newby, Laurel M. ; Kulkarni, Shankar J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4308-87da131533e5e5949790ff2e882f8097475ca0037bbe34b365999acb8693be983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biochemistry. Physiology. Immunology</topic><topic>Biological and medical sciences</topic><topic>Central Nervous System - metabolism</topic><topic>Chromosome Deletion</topic><topic>Cloning, Molecular</topic><topic>Diptera</topic><topic>DNA - genetics</topic><topic>DOPA decarboxylase</topic><topic>Dopa Decarboxylase - genetics</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophila melanogaster - physiology</topic><topic>Drosophilidae</topic><topic>Forecasting</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma-Aminobutyric Acid - physiology</topic><topic>Gene</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Glutamate Decarboxylase - genetics</topic><topic>Glutamate Decarboxylase - metabolism</topic><topic>Glutamic acid decarboxylase</topic><topic>Immunologic Techniques</topic><topic>inhibitory</topic><topic>Insect</topic><topic>Insecta</topic><topic>Invertebrates</topic><topic>Molecular Sequence Data</topic><topic>Neurotransmitters</topic><topic>Physiology. Development</topic><topic>Protein Biosynthesis</topic><topic>RNA - genetics</topic><topic>RNA, Complementary</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jackson, F. Rob</creatorcontrib><creatorcontrib>Newby, Laurel M.</creatorcontrib><creatorcontrib>Kulkarni, Shankar J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jackson, F. Rob</au><au>Newby, Laurel M.</au><au>Kulkarni, Shankar J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Drosophila GABAergic Systems: Sequence and Expression of Glutamic Acid Decarboxylase</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1990-03</date><risdate>1990</risdate><volume>54</volume><issue>3</issue><spage>1068</spage><epage>1078</epage><pages>1068-1078</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: A mammalian glutamic acid decarboxylase (GAD) cDNA probe has been utilized to isolate Drosophila cDNA clones that represent a genomic locus in chromosome region 64A. Deletion analysis indicates that this chromosomal locus encodes an enzymatically active GAD protein. The in vitro translation of cRNA representing a Drosophila cDNA clone yields a 57‐kDa protein that can be immunoprecipitated by an anti‐GAD antiserum. A GAD‐immunoreactive protein of the same size can also be detected in Drosophila head extracts. The nucleotide sequence derived from two overlapping Drosophila cDNA clones predicts a 57,759‐dalton protein composed of 510 residues that is 53% identical to mammalian GAD. Sequence comparisons of mammalian and Drosophila GAD identify two highly conserved regions (≥70% identity), one of which encompasses a putative cofactor‐binding domain. Transcriptional analyses show that expression of the Drosophila Gad gene commences early in embryonic development (4–8 h) and continues in all later developmental stages. A 3.1‐kb class of mRNA is detected throughout embryogenesis, in all three larval stages, in pupae, and in adults. This transcript class has a widespread distribution in the adult CNS. A smaller 2.6‐kb transcript is expressed in a developmentally regulated manner; it is detected only in embryos and pupae.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>1689376</pmid><doi>10.1111/j.1471-4159.1990.tb02359.x</doi><tpages>11</tpages></addata></record> |
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| ispartof | Journal of neurochemistry, 1990-03, Vol.54 (3), p.1068-1078 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Amino Acid Sequence Animals Base Sequence Biochemistry. Physiology. Immunology Biological and medical sciences Central Nervous System - metabolism Chromosome Deletion Cloning, Molecular Diptera DNA - genetics DOPA decarboxylase Dopa Decarboxylase - genetics Drosophila melanogaster Drosophila melanogaster - genetics Drosophila melanogaster - physiology Drosophilidae Forecasting Fundamental and applied biological sciences. Psychology gamma-Aminobutyric Acid - physiology Gene Gene Expression Regulation, Enzymologic Glutamate Decarboxylase - genetics Glutamate Decarboxylase - metabolism Glutamic acid decarboxylase Immunologic Techniques inhibitory Insect Insecta Invertebrates Molecular Sequence Data Neurotransmitters Physiology. Development Protein Biosynthesis RNA - genetics RNA, Complementary Transcription, Genetic |
| title | Drosophila GABAergic Systems: Sequence and Expression of Glutamic Acid Decarboxylase |
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