Immunoreactivity of anti K562 monoclonal antibodies with leukemic cells of myeloid lineage
Three monoclonal antibodies (MAb) raised against K562 cells (erythroleukemic cell line) reacting specifically with myeloid maturation related antigens, were characterized. MAb 4.3 (IgG2bk) reacted with promyelocytes, metamyelocytes and myelocytes, MAb 4.6 (IgG3k) also identified the myeloid blasts,...
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| Veröffentlicht in: | Leukemia research 1990, Vol.14 (1), p.69-75 |
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| container_title | Leukemia research |
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| creator | Chiplunkar, Shubhada V. Maniar, Hina S. Tatake, Revati J. Amin, Kishore M. Advani, Suresh H. Gangal, Sudha G. |
| description | Three monoclonal antibodies (MAb) raised against K562 cells (erythroleukemic cell line) reacting specifically with myeloid maturation related antigens, were characterized. MAb 4.3 (IgG2bk) reacted with promyelocytes, metamyelocytes and myelocytes, MAb 4.6 (IgG3k) also identified the myeloid blasts, while MAb 4.8 (IgG1k) reacted with metamyelocytes. The affinity constant of the MAbs ranged from 0.8 × 10
7–3.9 × 10
8 M
−1 and the binding sites on K562 cells varied from 8 × 10
4–4 × 10
5. In competition RIA MAbs 4.6 and 4.8 competed with each other for binding sites on K562 cells. In Western blot analysis the MAbs reacted with 66,000 mol.wt and 84,000 mol.wt peptides on K562 cells and 97,000 mol.wt peptide on chronic myeloid leukemia (CML) cells. These MAbs may help in immunophenotyping of myeloid leukemias. |
| doi_str_mv | 10.1016/0145-2126(90)90148-3 |
| format | Article |
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7–3.9 × 10
8 M
−1 and the binding sites on K562 cells varied from 8 × 10
4–4 × 10
5. In competition RIA MAbs 4.6 and 4.8 competed with each other for binding sites on K562 cells. In Western blot analysis the MAbs reacted with 66,000 mol.wt and 84,000 mol.wt peptides on K562 cells and 97,000 mol.wt peptide on chronic myeloid leukemia (CML) cells. These MAbs may help in immunophenotyping of myeloid leukemias.</description><identifier>ISSN: 0145-2126</identifier><identifier>EISSN: 1873-5835</identifier><identifier>DOI: 10.1016/0145-2126(90)90148-3</identifier><identifier>PMID: 1689437</identifier><identifier>CODEN: LEREDD</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Antibodies, Monoclonal - biosynthesis ; Antibodies, Monoclonal - immunology ; Antigen-Antibody Reactions ; Antigen-antibody reactions, antigen-antibody complexes, antibody-complement and others. Study of affinity. Antigen presentation ; Antigens, Neoplasm - analysis ; Antigens, Neoplasm - immunology ; Binding Sites, Antibody ; Binding, Competitive ; Biological and medical sciences ; Blotting, Western ; Cross Reactions ; Epitopes - analysis ; Epitopes - immunology ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Immunoenzyme Techniques ; K562 cells ; Leukemia, Erythroblastic, Acute - immunology ; Leukemia, Myeloid - immunology ; Mice ; Mice, Inbred BALB C ; Molecular immunology ; Molecular Weight ; Monoclonal antibodies ; myeloid leukemia cells</subject><ispartof>Leukemia research, 1990, Vol.14 (1), p.69-75</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c336t-2eb71108e7be8e0e392ab62122e087fdd0553940bd916bb670a6ca81697a1f803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0145212690901483$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19436179$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1689437$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chiplunkar, Shubhada V.</creatorcontrib><creatorcontrib>Maniar, Hina S.</creatorcontrib><creatorcontrib>Tatake, Revati J.</creatorcontrib><creatorcontrib>Amin, Kishore M.</creatorcontrib><creatorcontrib>Advani, Suresh H.</creatorcontrib><creatorcontrib>Gangal, Sudha G.</creatorcontrib><title>Immunoreactivity of anti K562 monoclonal antibodies with leukemic cells of myeloid lineage</title><title>Leukemia research</title><addtitle>Leuk Res</addtitle><description>Three monoclonal antibodies (MAb) raised against K562 cells (erythroleukemic cell line) reacting specifically with myeloid maturation related antigens, were characterized. MAb 4.3 (IgG2bk) reacted with promyelocytes, metamyelocytes and myelocytes, MAb 4.6 (IgG3k) also identified the myeloid blasts, while MAb 4.8 (IgG1k) reacted with metamyelocytes. The affinity constant of the MAbs ranged from 0.8 × 10
7–3.9 × 10
8 M
−1 and the binding sites on K562 cells varied from 8 × 10
4–4 × 10
5. In competition RIA MAbs 4.6 and 4.8 competed with each other for binding sites on K562 cells. In Western blot analysis the MAbs reacted with 66,000 mol.wt and 84,000 mol.wt peptides on K562 cells and 97,000 mol.wt peptide on chronic myeloid leukemia (CML) cells. These MAbs may help in immunophenotyping of myeloid leukemias.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigen-Antibody Reactions</subject><subject>Antigen-antibody reactions, antigen-antibody complexes, antibody-complement and others. Study of affinity. Antigen presentation</subject><subject>Antigens, Neoplasm - analysis</subject><subject>Antigens, Neoplasm - immunology</subject><subject>Binding Sites, Antibody</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cross Reactions</subject><subject>Epitopes - analysis</subject><subject>Epitopes - immunology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>K562 cells</subject><subject>Leukemia, Erythroblastic, Acute - immunology</subject><subject>Leukemia, Myeloid - immunology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular immunology</subject><subject>Molecular Weight</subject><subject>Monoclonal antibodies</subject><subject>myeloid leukemia cells</subject><issn>0145-2126</issn><issn>1873-5835</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1O3DAUha0KBMO0bwBSNiC6CL2OE_9skNCoP4iR2LSbbizHuQGDEw92QjVv34QZlV1Xln2_c330EXJK4YoC5V-AllVe0IJfKvisppvM2QeyoFKwvJKsOiCLf8gxOUnpCQAqRdUROaJcqpKJBfl923VjHyIaO7hXN2yz0GamH1x2V_Ei60IfrA-98W-PdWgcpuyPGx4zj-Mzds5mFr1Pc6zbog-uybzr0TzgR3LYGp_w0_5ckl_fvv5c_cjX999vVzfr3DLGh7zAWlAKEkWNEgGZKkzNp9IFghRt00BVMVVC3SjK65oLMNwaSbkShrYS2JJc7PZuYngZMQ26c2kuZXoMY9JC8QIEUxNY7kAbQ0oRW72JrjNxqynoWamefenZl1ag35RqNsXO9vvHusPmPbRzOM3P93OTrPFtNL116R2bIE7F_P31jsNJxqvDqJN12FtsXEQ76Ca4_xf5C8qrkgE</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Chiplunkar, Shubhada V.</creator><creator>Maniar, Hina S.</creator><creator>Tatake, Revati J.</creator><creator>Amin, Kishore M.</creator><creator>Advani, Suresh H.</creator><creator>Gangal, Sudha G.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>Immunoreactivity of anti K562 monoclonal antibodies with leukemic cells of myeloid lineage</title><author>Chiplunkar, Shubhada V. ; Maniar, Hina S. ; Tatake, Revati J. ; Amin, Kishore M. ; Advani, Suresh H. ; Gangal, Sudha G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c336t-2eb71108e7be8e0e392ab62122e087fdd0553940bd916bb670a6ca81697a1f803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigen-Antibody Reactions</topic><topic>Antigen-antibody reactions, antigen-antibody complexes, antibody-complement and others. Study of affinity. Antigen presentation</topic><topic>Antigens, Neoplasm - analysis</topic><topic>Antigens, Neoplasm - immunology</topic><topic>Binding Sites, Antibody</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cross Reactions</topic><topic>Epitopes - analysis</topic><topic>Epitopes - immunology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>K562 cells</topic><topic>Leukemia, Erythroblastic, Acute - immunology</topic><topic>Leukemia, Myeloid - immunology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular immunology</topic><topic>Molecular Weight</topic><topic>Monoclonal antibodies</topic><topic>myeloid leukemia cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chiplunkar, Shubhada V.</creatorcontrib><creatorcontrib>Maniar, Hina S.</creatorcontrib><creatorcontrib>Tatake, Revati J.</creatorcontrib><creatorcontrib>Amin, Kishore M.</creatorcontrib><creatorcontrib>Advani, Suresh H.</creatorcontrib><creatorcontrib>Gangal, Sudha G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Leukemia research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chiplunkar, Shubhada V.</au><au>Maniar, Hina S.</au><au>Tatake, Revati J.</au><au>Amin, Kishore M.</au><au>Advani, Suresh H.</au><au>Gangal, Sudha G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunoreactivity of anti K562 monoclonal antibodies with leukemic cells of myeloid lineage</atitle><jtitle>Leukemia research</jtitle><addtitle>Leuk Res</addtitle><date>1990</date><risdate>1990</risdate><volume>14</volume><issue>1</issue><spage>69</spage><epage>75</epage><pages>69-75</pages><issn>0145-2126</issn><eissn>1873-5835</eissn><coden>LEREDD</coden><abstract>Three monoclonal antibodies (MAb) raised against K562 cells (erythroleukemic cell line) reacting specifically with myeloid maturation related antigens, were characterized. MAb 4.3 (IgG2bk) reacted with promyelocytes, metamyelocytes and myelocytes, MAb 4.6 (IgG3k) also identified the myeloid blasts, while MAb 4.8 (IgG1k) reacted with metamyelocytes. The affinity constant of the MAbs ranged from 0.8 × 10
7–3.9 × 10
8 M
−1 and the binding sites on K562 cells varied from 8 × 10
4–4 × 10
5. In competition RIA MAbs 4.6 and 4.8 competed with each other for binding sites on K562 cells. In Western blot analysis the MAbs reacted with 66,000 mol.wt and 84,000 mol.wt peptides on K562 cells and 97,000 mol.wt peptide on chronic myeloid leukemia (CML) cells. These MAbs may help in immunophenotyping of myeloid leukemias.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>1689437</pmid><doi>10.1016/0145-2126(90)90148-3</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antigen-Antibody Reactions Antigen-antibody reactions, antigen-antibody complexes, antibody-complement and others. Study of affinity. Antigen presentation Antigens, Neoplasm - analysis Antigens, Neoplasm - immunology Binding Sites, Antibody Binding, Competitive Biological and medical sciences Blotting, Western Cross Reactions Epitopes - analysis Epitopes - immunology Female Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunoenzyme Techniques K562 cells Leukemia, Erythroblastic, Acute - immunology Leukemia, Myeloid - immunology Mice Mice, Inbred BALB C Molecular immunology Molecular Weight Monoclonal antibodies myeloid leukemia cells |
| title | Immunoreactivity of anti K562 monoclonal antibodies with leukemic cells of myeloid lineage |
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