Quantitative Measurement for Endothelial Constitutive Nitric Oxide Synthase in Cultured Human Endothelial Cells

Constitutively expressed endothelial nitric oxide synthase (ecNOS) produces nitric oxide (NO) froml-arginine and is important for the maintenance of cardiovascular homeostasis. We report the development of a capture ELISA which is specific for ecNOS. The assay detection limit is 0.5 ng/ml ecNOS prot...

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Veröffentlicht in:	Nitric oxide 1997-06, Vol.1 (3), p.226-233
Hauptverfasser:	Aberle, Sharon, Young, Tish Ann, Medberry, Poonam, Parkinson, John, Rubanyi, Gabor M., Kauser, Katalin
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Cells, Cultured CHO Cells Cricetinae Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Endothelium, Vascular - enzymology Enzyme-Linked Immunosorbent Assay Humans Nitric Oxide Synthase - metabolism Nitric Oxide Synthase Type III Sensitivity and Specificity Tumor Necrosis Factor-alpha - pharmacology
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