Rapid detection of deletions in the Duchenne muscular dystrophy gene by PCR amplification of deletion-prone exon sequences

Using the polymerase chain reaction (PCR) technique, we have screened the DNA of 42 patients with Duchenne or Becker muscular dystrophy for deletions within the DMD gene. Two regions within putative deletion "hot spots" of this gene were tested, and deletions were found in 16.6% of patient...

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Veröffentlicht in:	Human genetics 1990-02, Vol.84 (3), p.228-232
Hauptverfasser:	Hentemann, M, Reiss, J, Wagner, M, Cooper, D N
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Blotting, Southern Chromosome Deletion DNA - genetics Duchenne's muscular dystrophy Exons gene amplification genes Humans Molecular Sequence Data Muscular Dystrophies - genetics Oligonucleotide Probes Polymerase Chain Reaction
Online-Zugang:	Volltext
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