G-DNA: A Twice-Folded DNA Structure Adopted by Single-Stranded Oligo(dG) and Its Implications for Telomeres
Our dimethyl sulfate modification experiments suggest that (dG)nstretches within single-stranded DNA fragments, which represent the simplest model for telomeric sequences, adopt a complex intrastrand structure other than a simple hairpin. We present a molecular model for the DNA structure that confo...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1990-02, Vol.87 (3), p.867-870 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Panyutin, I. G. Kovalsky, O. I. Budowsky, E. I. Dickerson, R. E. Rikhirev, M. E. Lipanov, A. A. |
| description | Our dimethyl sulfate modification experiments suggest that (dG)nstretches within single-stranded DNA fragments, which represent the simplest model for telomeric sequences, adopt a complex intrastrand structure other than a simple hairpin. We present a molecular model for the DNA structure that conforms to dimethyl sulfate methylation data. The principal element of this G-DNA structure is a quadruple helix formed by pairwise antiparallel segments of the twice-folded (dG)nstretch. This quadruple core has two wide and two narrow grooves connected by three loop-shaped segments. The strong stacking interactions of the neighboring guanine tetrads and the large number of hydrogen bonds formed can be the primary reasons that such structures are favored over a common hairpin for long (dG)nstretches. Such compact structures may be formed from (dG)nstretches of telomeric sequences. |
| doi_str_mv | 10.1073/pnas.87.3.867 |
| format | Article |
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The strong stacking interactions of the neighboring guanine tetrads and the large number of hydrogen bonds formed can be the primary reasons that such structures are favored over a common hairpin for long (dG)nstretches. Such compact structures may be formed from (dG)nstretches of telomeric sequences.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.87.3.867</identifier><identifier>PMID: 2300578</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chemical bases ; Chromosomes - ultrastructure ; DNA ; Dna, deoxyribonucleoproteins ; Fundamental and applied biological sciences. 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G.</creatorcontrib><creatorcontrib>Kovalsky, O. I.</creatorcontrib><creatorcontrib>Budowsky, E. I.</creatorcontrib><creatorcontrib>Dickerson, R. E.</creatorcontrib><creatorcontrib>Rikhirev, M. E.</creatorcontrib><creatorcontrib>Lipanov, A. A.</creatorcontrib><title>G-DNA: A Twice-Folded DNA Structure Adopted by Single-Stranded Oligo(dG) and Its Implications for Telomeres</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Our dimethyl sulfate modification experiments suggest that (dG)nstretches within single-stranded DNA fragments, which represent the simplest model for telomeric sequences, adopt a complex intrastrand structure other than a simple hairpin. We present a molecular model for the DNA structure that conforms to dimethyl sulfate methylation data. The principal element of this G-DNA structure is a quadruple helix formed by pairwise antiparallel segments of the twice-folded (dG)nstretch. This quadruple core has two wide and two narrow grooves connected by three loop-shaped segments. The strong stacking interactions of the neighboring guanine tetrads and the large number of hydrogen bonds formed can be the primary reasons that such structures are favored over a common hairpin for long (dG)nstretches. Such compact structures may be formed from (dG)nstretches of telomeric sequences.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chemical bases</subject><subject>Chromosomes - ultrastructure</subject><subject>DNA</subject><subject>Dna, deoxyribonucleoproteins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Hydrogen Bonding</subject><subject>Hydrogen bonds</subject><subject>Methylation</subject><subject>Models, Molecular</subject><subject>Molecular structure</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic acids</subject><subject>Oligodeoxyribonucleotides</subject><subject>Oligomers</subject><subject>Sulfates</subject><subject>Telomeres</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9v0zAcxS0EGt3gyA0kXzbBIcWO41-ISzVYqTSxw8rZSuNvSoYbB9sB9t_jamErFzhZ_r7P9_lZD6EXlMwpkezt0NdxruSczZWQj9CMEk0LUWnyGM0IKWWhqrJ6io5jvCGEaK7IEToqGSFcqhn6tiw-fF68wwu8_tk1UFx4Z8HiPMPXKYxNGgPghfVDytPNLb7u-q2DImt1vwevXLf1r-3yDc53vEoRr3aD65o6db6PuPUBr8H5HQSIz9CTtnYRnk_nCfpy8XF9_qm4vFquzheXRcOlToVSilALFSlJrTWzUAIjVomylUAqwWmpQHLR6g1tW8WE1VpZSUthubIVB3aC3t_5DuNmB7aBPqd1Zgjdrg63xted-Vvpu69m638YzpjQef1sWg_--wgxmV0XG3Cu7sGP0UgtCGVC_BekXFCuuMpgcQc2wccYoL3PQonZl2j2JRolDTO5xMy_OvzAPT21lvXTSa9jU7s2d9F08cFUVyXTssrcy4nb2_-RD545_Yds2tG5BL_Sg81NTD4cpOFMlpL9Bu_wxKc</recordid><startdate>19900201</startdate><enddate>19900201</enddate><creator>Panyutin, I. G.</creator><creator>Kovalsky, O. I.</creator><creator>Budowsky, E. I.</creator><creator>Dickerson, R. E.</creator><creator>Rikhirev, M. E.</creator><creator>Lipanov, A. A.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19900201</creationdate><title>G-DNA: A Twice-Folded DNA Structure Adopted by Single-Stranded Oligo(dG) and Its Implications for Telomeres</title><author>Panyutin, I. G. ; Kovalsky, O. I. ; Budowsky, E. I. ; Dickerson, R. E. ; Rikhirev, M. E. ; Lipanov, A. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c579t-88801de4020a993de2e30d862f7e0465128e756f9b1ff836d998d7126d58d45e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chemical bases</topic><topic>Chromosomes - ultrastructure</topic><topic>DNA</topic><topic>Dna, deoxyribonucleoproteins</topic><topic>Fundamental and applied biological sciences. 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The principal element of this G-DNA structure is a quadruple helix formed by pairwise antiparallel segments of the twice-folded (dG)nstretch. This quadruple core has two wide and two narrow grooves connected by three loop-shaped segments. The strong stacking interactions of the neighboring guanine tetrads and the large number of hydrogen bonds formed can be the primary reasons that such structures are favored over a common hairpin for long (dG)nstretches. Such compact structures may be formed from (dG)nstretches of telomeric sequences.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2300578</pmid><doi>10.1073/pnas.87.3.867</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1990-02, Vol.87 (3), p.867-870 |
| issn | 0027-8424 1091-6490 |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Chemical bases Chromosomes - ultrastructure DNA Dna, deoxyribonucleoproteins Fundamental and applied biological sciences. Psychology Gels Hydrogen Bonding Hydrogen bonds Methylation Models, Molecular Molecular structure Nucleic Acid Conformation Nucleic acids Oligodeoxyribonucleotides Oligomers Sulfates Telomeres |
| title | G-DNA: A Twice-Folded DNA Structure Adopted by Single-Stranded Oligo(dG) and Its Implications for Telomeres |
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