Molecular Relapse in Chronic Myelogenous Leukemia Patients After Bone Marrow Transplantation Detected by Polymerase Chain Reaction
Relapse of chronic myelogenous leukemia after bone marrow transplantation can be detected by using clinical, cytogenetic, or molecular tools. A modification of the polymerase chain reaction can be used in patients to detect low levels of the BCR-ABL-encoded mRNA transcript, a specific marker for chr...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1990-01, Vol.87 (2), p.563-567 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Sawyers, Charles L. Timson, Lisa Kawasaki, Ernest S. Clark, Steven S. Witte, Owen N. Champlin, Richard |
| description | Relapse of chronic myelogenous leukemia after bone marrow transplantation can be detected by using clinical, cytogenetic, or molecular tools. A modification of the polymerase chain reaction can be used in patients to detect low levels of the BCR-ABL-encoded mRNA transcript, a specific marker for chronic myelogenous leukemia. Early detection of relapse after bone marrow transplantation could potentially alter treatment decisions. We prospectively evaluated 19 patients for evidence of molecular relapse, cytogenetic relapse, and clinical relapse after bone marrow transplantation. We used the polymerase chain reaction to detect residual BCR-ABL mRNA in patients followed up to 45 months after treatment (median, 15 months; range, 6-45 months) and found 4 patients with BCR-ABL mRNA expression following bone marrow transplantation. In 2 patients BCR-ABL mRNA was detected in all samples, and both have developed cytogenetic relapse. In 1 patient BCR-ABL mRNA was detected transiently during the first month after transplant but was undetectable thereafter. The fourth patient had BCR-ABL mRNA 6 months after bone marrow transplantation but not in prior samples. Fifteen patients did not express detectable BCR-ABL mRNA. All 19 patients remain in clinical remission. In this prospective study of chronic myelogenous leukemia patients treated with bone marrow transplantation, molecular relapse preceded cytogenetic relapse in those patients who persistently express BCR-ABL mRNA. We recommend using standard clinical and cytogenetic testing to make patient care decisions until further follow-up determines the clinical outcome of those patients with residual BCR-ABL mRNA transcripts detected by polymerase chain reaction. |
| doi_str_mv | 10.1073/pnas.87.2.563 |
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A modification of the polymerase chain reaction can be used in patients to detect low levels of the BCR-ABL-encoded mRNA transcript, a specific marker for chronic myelogenous leukemia. Early detection of relapse after bone marrow transplantation could potentially alter treatment decisions. We prospectively evaluated 19 patients for evidence of molecular relapse, cytogenetic relapse, and clinical relapse after bone marrow transplantation. We used the polymerase chain reaction to detect residual BCR-ABL mRNA in patients followed up to 45 months after treatment (median, 15 months; range, 6-45 months) and found 4 patients with BCR-ABL mRNA expression following bone marrow transplantation. In 2 patients BCR-ABL mRNA was detected in all samples, and both have developed cytogenetic relapse. In 1 patient BCR-ABL mRNA was detected transiently during the first month after transplant but was undetectable thereafter. The fourth patient had BCR-ABL mRNA 6 months after bone marrow transplantation but not in prior samples. Fifteen patients did not express detectable BCR-ABL mRNA. All 19 patients remain in clinical remission. In this prospective study of chronic myelogenous leukemia patients treated with bone marrow transplantation, molecular relapse preceded cytogenetic relapse in those patients who persistently express BCR-ABL mRNA. We recommend using standard clinical and cytogenetic testing to make patient care decisions until further follow-up determines the clinical outcome of those patients with residual BCR-ABL mRNA transcripts detected by polymerase chain reaction.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.87.2.563</identifier><identifier>PMID: 2405384</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>550401 - Genetics- Tracer Techniques ; ANIMAL CELLS ; ANIMAL TISSUES ; Base Sequence ; BASIC BIOLOGICAL SCIENCES ; BIOCHEMISTRY ; Biological and medical sciences ; Biomarkers, Tumor - analysis ; BIOSYNTHESIS ; Blast Crisis - diagnosis ; Blood ; BODY ; BONE MARROW ; BONE MARROW CELLS ; Bone Marrow Transplantation ; CHEMISTRY ; CHROMOSOMES ; Chronic myeloid leukemia ; CONNECTIVE TISSUE CELLS ; CYTOCHEMISTRY ; Cytogenetics ; DISEASES ; DNA POLYMERASES ; ENZYMES ; Follow-Up Studies ; Fusion Proteins, bcr-abl - genetics ; GENE AMPLIFICATION ; Hematologic and hematopoietic diseases ; HEMATOPOIETIC SYSTEM ; HEMIC DISEASES ; Humans ; IMMUNE SYSTEM DISEASES ; LEUKEMIA ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - pathology ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - surgery ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Medical sciences ; MESSENGER-RNA ; Metaphase ; Molecular Sequence Data ; NEOPLASMS ; Nucleic Acid Amplification Techniques ; NUCLEIC ACIDS ; NUCLEOTIDYLTRANSFERASES ; Oligonucleotide Probes ; OLIGONUCLEOTIDES ; ORGANIC COMPOUNDS ; ORGANS ; PATIENTS ; PHILADELPHIA CHROMOSOME ; PHOSPHORUS-GROUP TRANSFERASES ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; POLYMERASES ; Recurrence ; Relapse ; RNA ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; SOMATIC CELLS ; SYNTHESIS ; Tissue grafting ; TISSUES ; TRANSCRIPTION ; TRANSFERASES ; TRANSPLANTS ; TUMOR CELLS</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1990-01, Vol.87 (2), p.563-567</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c568t-978371d4c038a1cc5f3dd8887610eb09eb6d58b021e01e650e6cbf2f489db1923</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/87/2.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2353550$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2353550$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,4024,27923,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6911504$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2405384$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/7063800$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Sawyers, Charles L.</creatorcontrib><creatorcontrib>Timson, Lisa</creatorcontrib><creatorcontrib>Kawasaki, Ernest S.</creatorcontrib><creatorcontrib>Clark, Steven S.</creatorcontrib><creatorcontrib>Witte, Owen N.</creatorcontrib><creatorcontrib>Champlin, Richard</creatorcontrib><title>Molecular Relapse in Chronic Myelogenous Leukemia Patients After Bone Marrow Transplantation Detected by Polymerase Chain Reaction</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Relapse of chronic myelogenous leukemia after bone marrow transplantation can be detected by using clinical, cytogenetic, or molecular tools. A modification of the polymerase chain reaction can be used in patients to detect low levels of the BCR-ABL-encoded mRNA transcript, a specific marker for chronic myelogenous leukemia. Early detection of relapse after bone marrow transplantation could potentially alter treatment decisions. We prospectively evaluated 19 patients for evidence of molecular relapse, cytogenetic relapse, and clinical relapse after bone marrow transplantation. We used the polymerase chain reaction to detect residual BCR-ABL mRNA in patients followed up to 45 months after treatment (median, 15 months; range, 6-45 months) and found 4 patients with BCR-ABL mRNA expression following bone marrow transplantation. In 2 patients BCR-ABL mRNA was detected in all samples, and both have developed cytogenetic relapse. In 1 patient BCR-ABL mRNA was detected transiently during the first month after transplant but was undetectable thereafter. The fourth patient had BCR-ABL mRNA 6 months after bone marrow transplantation but not in prior samples. Fifteen patients did not express detectable BCR-ABL mRNA. All 19 patients remain in clinical remission. In this prospective study of chronic myelogenous leukemia patients treated with bone marrow transplantation, molecular relapse preceded cytogenetic relapse in those patients who persistently express BCR-ABL mRNA. We recommend using standard clinical and cytogenetic testing to make patient care decisions until further follow-up determines the clinical outcome of those patients with residual BCR-ABL mRNA transcripts detected by polymerase chain reaction.</description><subject>550401 - Genetics- Tracer Techniques</subject><subject>ANIMAL CELLS</subject><subject>ANIMAL TISSUES</subject><subject>Base Sequence</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BIOCHEMISTRY</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - analysis</subject><subject>BIOSYNTHESIS</subject><subject>Blast Crisis - diagnosis</subject><subject>Blood</subject><subject>BODY</subject><subject>BONE MARROW</subject><subject>BONE MARROW CELLS</subject><subject>Bone Marrow Transplantation</subject><subject>CHEMISTRY</subject><subject>CHROMOSOMES</subject><subject>Chronic myeloid leukemia</subject><subject>CONNECTIVE TISSUE CELLS</subject><subject>CYTOCHEMISTRY</subject><subject>Cytogenetics</subject><subject>DISEASES</subject><subject>DNA POLYMERASES</subject><subject>ENZYMES</subject><subject>Follow-Up Studies</subject><subject>Fusion Proteins, bcr-abl - genetics</subject><subject>GENE AMPLIFICATION</subject><subject>Hematologic and hematopoietic diseases</subject><subject>HEMATOPOIETIC SYSTEM</subject><subject>HEMIC DISEASES</subject><subject>Humans</subject><subject>IMMUNE SYSTEM DISEASES</subject><subject>LEUKEMIA</subject><subject>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics</subject><subject>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - pathology</subject><subject>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - surgery</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Medical sciences</subject><subject>MESSENGER-RNA</subject><subject>Metaphase</subject><subject>Molecular Sequence Data</subject><subject>NEOPLASMS</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>NUCLEIC ACIDS</subject><subject>NUCLEOTIDYLTRANSFERASES</subject><subject>Oligonucleotide Probes</subject><subject>OLIGONUCLEOTIDES</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANS</subject><subject>PATIENTS</subject><subject>PHILADELPHIA CHROMOSOME</subject><subject>PHOSPHORUS-GROUP TRANSFERASES</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>POLYMERASES</subject><subject>Recurrence</subject><subject>Relapse</subject><subject>RNA</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>SOMATIC CELLS</subject><subject>SYNTHESIS</subject><subject>Tissue grafting</subject><subject>TISSUES</subject><subject>TRANSCRIPTION</subject><subject>TRANSFERASES</subject><subject>TRANSPLANTS</subject><subject>TUMOR CELLS</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1vEzEQxVcIVELgyA0kCwG3DeP12uuVuJTwKaWiqsrZ8npnmy2OHWwHyJW_HEcJabnAyYf387x5M1MUjynMKDTs1drpOJPNrJpxwe4UEwotLUXdwt1iAlA1payr-n7xIMZrAGi5hJPipKqBM1lPil9n3qLZWB3IBVq9jkhGR-bL4N1oyNkWrb9C5zeRLHDzFVejJuc6jehSJKdDwkDeeIfkTIfgf5DLoF1cW-1SZrwjbzGhSdiTbkvOvd2uMOjsMF_qbHKB2uyoh8W9QduIjw7vtPjy_t3l_GO5-Pzh0_x0URouZCrbRrKG9rUBJjU1hg-s76WUjaCAHbTYiZ7LDiqKQFFwQGG6oRpq2fYdbSs2LV7v66433Qp7kzMEbdU6jCsdtsrrUf2tuHGprvx3xRnL05oWz_bffUyjimbM0ZbGO5cTqgYEkwAZennwCP7bBmNSqzEatHkkmIeomlZkSrb_BSlnbS1Ek8FyD5rgYww4HBumoHYHoHYHoGSjKpUPIPNPb6c80oeNZ_35QdfRaDvkjZkxHjHRUsphhz05YLvqf9RbLi_-IathY23Cn-mmzHVMPtw0wzjjHNhv55Hb5A</recordid><startdate>19900101</startdate><enddate>19900101</enddate><creator>Sawyers, Charles L.</creator><creator>Timson, Lisa</creator><creator>Kawasaki, Ernest S.</creator><creator>Clark, Steven S.</creator><creator>Witte, Owen N.</creator><creator>Champlin, Richard</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T3</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19900101</creationdate><title>Molecular Relapse in Chronic Myelogenous Leukemia Patients After Bone Marrow Transplantation Detected by Polymerase Chain Reaction</title><author>Sawyers, Charles L. ; Timson, Lisa ; Kawasaki, Ernest S. ; Clark, Steven S. ; Witte, Owen N. ; Champlin, Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c568t-978371d4c038a1cc5f3dd8887610eb09eb6d58b021e01e650e6cbf2f489db1923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>550401 - Genetics- Tracer Techniques</topic><topic>ANIMAL CELLS</topic><topic>ANIMAL TISSUES</topic><topic>Base Sequence</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BIOCHEMISTRY</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - analysis</topic><topic>BIOSYNTHESIS</topic><topic>Blast Crisis - diagnosis</topic><topic>Blood</topic><topic>BODY</topic><topic>BONE MARROW</topic><topic>BONE MARROW CELLS</topic><topic>Bone Marrow Transplantation</topic><topic>CHEMISTRY</topic><topic>CHROMOSOMES</topic><topic>Chronic myeloid leukemia</topic><topic>CONNECTIVE TISSUE CELLS</topic><topic>CYTOCHEMISTRY</topic><topic>Cytogenetics</topic><topic>DISEASES</topic><topic>DNA POLYMERASES</topic><topic>ENZYMES</topic><topic>Follow-Up Studies</topic><topic>Fusion Proteins, bcr-abl - genetics</topic><topic>GENE AMPLIFICATION</topic><topic>Hematologic and hematopoietic diseases</topic><topic>HEMATOPOIETIC SYSTEM</topic><topic>HEMIC DISEASES</topic><topic>Humans</topic><topic>IMMUNE SYSTEM DISEASES</topic><topic>LEUKEMIA</topic><topic>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics</topic><topic>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - pathology</topic><topic>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - surgery</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Medical sciences</topic><topic>MESSENGER-RNA</topic><topic>Metaphase</topic><topic>Molecular Sequence Data</topic><topic>NEOPLASMS</topic><topic>Nucleic Acid Amplification Techniques</topic><topic>NUCLEIC ACIDS</topic><topic>NUCLEOTIDYLTRANSFERASES</topic><topic>Oligonucleotide Probes</topic><topic>OLIGONUCLEOTIDES</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANS</topic><topic>PATIENTS</topic><topic>PHILADELPHIA CHROMOSOME</topic><topic>PHOSPHORUS-GROUP TRANSFERASES</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>POLYMERASES</topic><topic>Recurrence</topic><topic>Relapse</topic><topic>RNA</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - genetics</topic><topic>SOMATIC CELLS</topic><topic>SYNTHESIS</topic><topic>Tissue grafting</topic><topic>TISSUES</topic><topic>TRANSCRIPTION</topic><topic>TRANSFERASES</topic><topic>TRANSPLANTS</topic><topic>TUMOR CELLS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sawyers, Charles L.</creatorcontrib><creatorcontrib>Timson, Lisa</creatorcontrib><creatorcontrib>Kawasaki, Ernest S.</creatorcontrib><creatorcontrib>Clark, Steven S.</creatorcontrib><creatorcontrib>Witte, Owen N.</creatorcontrib><creatorcontrib>Champlin, Richard</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Human Genome Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sawyers, Charles L.</au><au>Timson, Lisa</au><au>Kawasaki, Ernest S.</au><au>Clark, Steven S.</au><au>Witte, Owen N.</au><au>Champlin, Richard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Relapse in Chronic Myelogenous Leukemia Patients After Bone Marrow Transplantation Detected by Polymerase Chain Reaction</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1990-01-01</date><risdate>1990</risdate><volume>87</volume><issue>2</issue><spage>563</spage><epage>567</epage><pages>563-567</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Relapse of chronic myelogenous leukemia after bone marrow transplantation can be detected by using clinical, cytogenetic, or molecular tools. A modification of the polymerase chain reaction can be used in patients to detect low levels of the BCR-ABL-encoded mRNA transcript, a specific marker for chronic myelogenous leukemia. Early detection of relapse after bone marrow transplantation could potentially alter treatment decisions. We prospectively evaluated 19 patients for evidence of molecular relapse, cytogenetic relapse, and clinical relapse after bone marrow transplantation. We used the polymerase chain reaction to detect residual BCR-ABL mRNA in patients followed up to 45 months after treatment (median, 15 months; range, 6-45 months) and found 4 patients with BCR-ABL mRNA expression following bone marrow transplantation. In 2 patients BCR-ABL mRNA was detected in all samples, and both have developed cytogenetic relapse. In 1 patient BCR-ABL mRNA was detected transiently during the first month after transplant but was undetectable thereafter. The fourth patient had BCR-ABL mRNA 6 months after bone marrow transplantation but not in prior samples. Fifteen patients did not express detectable BCR-ABL mRNA. All 19 patients remain in clinical remission. In this prospective study of chronic myelogenous leukemia patients treated with bone marrow transplantation, molecular relapse preceded cytogenetic relapse in those patients who persistently express BCR-ABL mRNA. We recommend using standard clinical and cytogenetic testing to make patient care decisions until further follow-up determines the clinical outcome of those patients with residual BCR-ABL mRNA transcripts detected by polymerase chain reaction.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2405384</pmid><doi>10.1073/pnas.87.2.563</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0027-8424 1091-6490 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 550401 - Genetics- Tracer Techniques ANIMAL CELLS ANIMAL TISSUES Base Sequence BASIC BIOLOGICAL SCIENCES BIOCHEMISTRY Biological and medical sciences Biomarkers, Tumor - analysis BIOSYNTHESIS Blast Crisis - diagnosis Blood BODY BONE MARROW BONE MARROW CELLS Bone Marrow Transplantation CHEMISTRY CHROMOSOMES Chronic myeloid leukemia CONNECTIVE TISSUE CELLS CYTOCHEMISTRY Cytogenetics DISEASES DNA POLYMERASES ENZYMES Follow-Up Studies Fusion Proteins, bcr-abl - genetics GENE AMPLIFICATION Hematologic and hematopoietic diseases HEMATOPOIETIC SYSTEM HEMIC DISEASES Humans IMMUNE SYSTEM DISEASES LEUKEMIA Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics Leukemia, Myelogenous, Chronic, BCR-ABL Positive - pathology Leukemia, Myelogenous, Chronic, BCR-ABL Positive - surgery Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Medical sciences MESSENGER-RNA Metaphase Molecular Sequence Data NEOPLASMS Nucleic Acid Amplification Techniques NUCLEIC ACIDS NUCLEOTIDYLTRANSFERASES Oligonucleotide Probes OLIGONUCLEOTIDES ORGANIC COMPOUNDS ORGANS PATIENTS PHILADELPHIA CHROMOSOME PHOSPHORUS-GROUP TRANSFERASES Polymerase chain reaction Polymerase Chain Reaction - methods POLYMERASES Recurrence Relapse RNA RNA, Messenger - analysis RNA, Messenger - genetics SOMATIC CELLS SYNTHESIS Tissue grafting TISSUES TRANSCRIPTION TRANSFERASES TRANSPLANTS TUMOR CELLS |
| title | Molecular Relapse in Chronic Myelogenous Leukemia Patients After Bone Marrow Transplantation Detected by Polymerase Chain Reaction |
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