Transfection of human lamins A and C into mouse embryonal carcinoma cells possessing only lamin B
The peripheral lamina of eukaryotic nuclei is composed of polypeptides called lamins that vary in number from one to four according to organism, cell type, and differentiated state of the cells. Early embryonic cells and stem cells of mammals generally possess only lamin B while lamins A and C appea...
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| Veröffentlicht in: | Experimental cell research 1990, Vol.186 (1), p.182-187 |
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| creator | Collard, Jean-François Raymond, Yves |
| description | The peripheral lamina of eukaryotic nuclei is composed of polypeptides called lamins that vary in number from one to four according to organism, cell type, and differentiated state of the cells. Early embryonic cells and stem cells of mammals generally possess only lamin B while lamins A and C appear later during differentiation. To study the role of the late appearance of lamins A and C in the differentiated phenotype, we have performed transfection of cDNAs coding for human lamins A or C into mouse embryonal carcinoma (EC) cell lines F9 and P19 lacking these two lamins. Transient transfections have shown that lamins A or C could be expressed, translocated to the peripheral lamina, and distributed into daughter cell nuclei after mitosis. These results demonstrated that EC cells devoid of lamins A and C nevertheless possessed the appropriate mechanisms for the localization and mitotic redistribution of exogenous lamins A and C. |
| doi_str_mv | 10.1016/0014-4827(90)90225-Y |
| format | Article |
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Early embryonic cells and stem cells of mammals generally possess only lamin B while lamins A and C appear later during differentiation. To study the role of the late appearance of lamins A and C in the differentiated phenotype, we have performed transfection of cDNAs coding for human lamins A or C into mouse embryonal carcinoma (EC) cell lines F9 and P19 lacking these two lamins. Transient transfections have shown that lamins A or C could be expressed, translocated to the peripheral lamina, and distributed into daughter cell nuclei after mitosis. 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Early embryonic cells and stem cells of mammals generally possess only lamin B while lamins A and C appear later during differentiation. To study the role of the late appearance of lamins A and C in the differentiated phenotype, we have performed transfection of cDNAs coding for human lamins A or C into mouse embryonal carcinoma (EC) cell lines F9 and P19 lacking these two lamins. Transient transfections have shown that lamins A or C could be expressed, translocated to the peripheral lamina, and distributed into daughter cell nuclei after mitosis. These results demonstrated that EC cells devoid of lamins A and C nevertheless possessed the appropriate mechanisms for the localization and mitotic redistribution of exogenous lamins A and C.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cell physiology</subject><subject>DNA - genetics</subject><subject>Embryonal Carcinoma Stem Cells</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lamin Type B</subject><subject>Lamins</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Neoplastic Stem Cells - metabolism</subject><subject>Neoplastic Stem Cells - pathology</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Transfection</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1rGzEQhkVpSdy0_yABHUppD9uOPtZaXQKp6RcEekkPOYmRVkpUdiVXsgP-95Vj42NPc5hnXl6eIeSSwScGbPkZgMlODlx90PBRA-d9d_-CLBho6Ljk_CVZnJBz8rrWPwAwDGx5Rs64BKkUWxC8K5hq8G4Tc6I50MftjIlOOMdU6Q3FNNIVjWmT6Zy31VM_27LLCSfqsLiY8ozU-WmqdJ1r9bXG9EBzmnaHDPrlDXkVcKr-7XFekN_fvt6tfnS3v77_XN3cdk4A23RqBGsVWKG1A-UQgsIh8CAtR8bBeiaWwgqLzEvrBejAxCg49tBbO9hRXJD3h9x1yX-3vm7MHOu-GSbfmhule61UrxooD6ArrXHxwaxLnLHsDAOzN2v22sxem9Fgns2a-3Z2dczf2tmPp6OjyrZ_d9xjdTiF5tXFesL6XopB6YZdHzDfXDxFX0x10Sfnx1jaF8yY4_97_AOV6ZVZ</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Collard, Jean-François</creator><creator>Raymond, Yves</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>Transfection of human lamins A and C into mouse embryonal carcinoma cells possessing only lamin B</title><author>Collard, Jean-François ; Raymond, Yves</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-7d0bb70b399c07ca0f7a8f2f4b2a120be1363b3ba1e4be309f13d32a505bb8bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cell physiology</topic><topic>DNA - genetics</topic><topic>Embryonal Carcinoma Stem Cells</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lamin Type B</topic><topic>Lamins</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Neoplastic Stem Cells - metabolism</topic><topic>Neoplastic Stem Cells - pathology</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Collard, Jean-François</creatorcontrib><creatorcontrib>Raymond, Yves</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Collard, Jean-François</au><au>Raymond, Yves</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transfection of human lamins A and C into mouse embryonal carcinoma cells possessing only lamin B</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1990</date><risdate>1990</risdate><volume>186</volume><issue>1</issue><spage>182</spage><epage>187</epage><pages>182-187</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><coden>ECREAL</coden><abstract>The peripheral lamina of eukaryotic nuclei is composed of polypeptides called lamins that vary in number from one to four according to organism, cell type, and differentiated state of the cells. Early embryonic cells and stem cells of mammals generally possess only lamin B while lamins A and C appear later during differentiation. To study the role of the late appearance of lamins A and C in the differentiated phenotype, we have performed transfection of cDNAs coding for human lamins A or C into mouse embryonal carcinoma (EC) cell lines F9 and P19 lacking these two lamins. Transient transfections have shown that lamins A or C could be expressed, translocated to the peripheral lamina, and distributed into daughter cell nuclei after mitosis. These results demonstrated that EC cells devoid of lamins A and C nevertheless possessed the appropriate mechanisms for the localization and mitotic redistribution of exogenous lamins A and C.</abstract><cop>Orlando, FL</cop><pub>Elsevier Inc</pub><pmid>2404771</pmid><doi>10.1016/0014-4827(90)90225-Y</doi><tpages>6</tpages></addata></record> |
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| ispartof | Experimental cell research, 1990, Vol.186 (1), p.182-187 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biological and medical sciences Cell Line Cell physiology DNA - genetics Embryonal Carcinoma Stem Cells Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Lamin Type B Lamins Mice Molecular and cellular biology Neoplastic Stem Cells - metabolism Neoplastic Stem Cells - pathology Nuclear Proteins - genetics Nuclear Proteins - metabolism Transfection |
| title | Transfection of human lamins A and C into mouse embryonal carcinoma cells possessing only lamin B |
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