S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factors
The S. cerevisiae RNA polymerase III (pol III) transcription factor TFIIIB binds to DNA upstream of the transcription start site of the SUP4 tRNATyr gene in a TFIIIC-dependent reaction and to the major 5S rRNA gene in a reaction requiring TFIIIC and TFIIIA. It is shown here that TFIIIB alone correct...
Gespeichert in:
| Veröffentlicht in: | Cell 1990-01, Vol.60 (2), p.235-245 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 245 |
|---|---|
| container_issue | 2 |
| container_start_page | 235 |
| container_title | Cell |
| container_volume | 60 |
| creator | Kassavetis, George A. Braun, Burkhard R. Nguyen, Lam H. Peter Geiduschek, E. |
| description | The S. cerevisiae RNA polymerase III (pol III) transcription factor TFIIIB binds to DNA upstream of the transcription start site of the SUP4 tRNATyr gene in a TFIIIC-dependent reaction and to the major 5S rRNA gene in a reaction requiring TFIIIC and TFIIIA. It is shown here that TFIIIB alone correctly positions pol III for repeated cycles of transcription on both genes, with the same efficiency as fully assembled transcription complexes. Thus, TFIIIB is the sole transcription initiation factor of S. cerevisiae pol III; TFIIIC and TFIIIA are assembly factors for TFIIIB. The TFIIIB-dependent binding of pol 111 to the SUP4 tRNA and 5S rRNA genes has been analyzed in binary (protein and DNA only) and precisely arrested ternary (protein, DNA, and RNA) transcription complexes. Pol III unwinds at least 14 by of DNA at the SUP4 transcription start in a temperature-dependent process. The unwound DNA segment moves downstream with nascent RNA as a transcription bubble of approximately the same size. |
| doi_str_mv | 10.1016/0092-8674(90)90739-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79593731</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0092867490907392</els_id><sourcerecordid>15459186</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-aa319c1d12760f2cb57e0063151da75332e3df4113baf23a76e90ec221a53e1b3</originalsourceid><addsrcrecordid>eNqFkc-KFDEQh4Mo6zj6Bgo5iCjYayrpdDoXYRxcHVgUdD2H6nQ1G-l_Jj0r8wI-tz07zRz1lIL66kdVPsaeg7gEAcU7IazMysLkr614Y4VRNpMP2AqENVkORj5kqzPymD1J6acQotRaX7ALmYu8AFixP98vuadIdyEFJH5ztdvtPvCQ-HRLfIrYJx_DOIWh56EPU8D7skE_DZGPcRgp8qHh375s-Di0h44iJuJzyFv--za0S-KGY1-fyi3HSBxToq5qD0tSesoeNdgmera8a_bj6uPN9nN2_fXTbru5zvx80ZQhKrAeapCmEI30lTYkRKFAQ41GKyVJ1U0OoCpspEJTkBXkpQTUiqBSa_bqlDuv_mtPaXJdSJ7aFnsa9skZq60yCv4Lgs61hbKYwfwE-jikFKlxYwwdxoMD4Y6e3FGCO0pwVrh7T07OYy-W_H3VUX0eWsTM_ZdLH5PHtplN-JDOWFGqspzvXbP3J4zmT7sLFF3ygXpPdYjkJ1cP4d97_AVtgKza</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15459186</pqid></control><display><type>article</type><title>S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factors</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Kassavetis, George A. ; Braun, Burkhard R. ; Nguyen, Lam H. ; Peter Geiduschek, E.</creator><creatorcontrib>Kassavetis, George A. ; Braun, Burkhard R. ; Nguyen, Lam H. ; Peter Geiduschek, E.</creatorcontrib><description>The S. cerevisiae RNA polymerase III (pol III) transcription factor TFIIIB binds to DNA upstream of the transcription start site of the SUP4 tRNATyr gene in a TFIIIC-dependent reaction and to the major 5S rRNA gene in a reaction requiring TFIIIC and TFIIIA. It is shown here that TFIIIB alone correctly positions pol III for repeated cycles of transcription on both genes, with the same efficiency as fully assembled transcription complexes. Thus, TFIIIB is the sole transcription initiation factor of S. cerevisiae pol III; TFIIIC and TFIIIA are assembly factors for TFIIIB. The TFIIIB-dependent binding of pol 111 to the SUP4 tRNA and 5S rRNA genes has been analyzed in binary (protein and DNA only) and precisely arrested ternary (protein, DNA, and RNA) transcription complexes. Pol III unwinds at least 14 by of DNA at the SUP4 transcription start in a temperature-dependent process. The unwound DNA segment moves downstream with nascent RNA as a transcription bubble of approximately the same size.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/0092-8674(90)90739-2</identifier><identifier>PMID: 2404611</identifier><identifier>CODEN: CELLB5</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Base Sequence ; Biological and medical sciences ; Deoxyribonuclease I ; DNA, Fungal - drug effects ; DNA, Fungal - genetics ; DNA-Directed RNA Polymerases - metabolism ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - metabolism ; Genes, Fungal ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Multigene Family ; Plasmids ; Potassium Permanganate - pharmacology ; RNA Polymerase III - metabolism ; RNA, Ribosomal, 5S - biosynthesis ; RNA, Ribosomal, 5S - genetics ; RNA, Transfer, Tyr - genetics ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Transcription Factor TFIIIA ; Transcription Factor TFIIIB ; Transcription Factors - isolation & purification ; Transcription Factors - metabolism ; Transcription Factors, TFIII ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. Rna processing</subject><ispartof>Cell, 1990-01, Vol.60 (2), p.235-245</ispartof><rights>1990</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-aa319c1d12760f2cb57e0063151da75332e3df4113baf23a76e90ec221a53e1b3</citedby><cites>FETCH-LOGICAL-c417t-aa319c1d12760f2cb57e0063151da75332e3df4113baf23a76e90ec221a53e1b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0092-8674(90)90739-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6838853$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2404611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kassavetis, George A.</creatorcontrib><creatorcontrib>Braun, Burkhard R.</creatorcontrib><creatorcontrib>Nguyen, Lam H.</creatorcontrib><creatorcontrib>Peter Geiduschek, E.</creatorcontrib><title>S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factors</title><title>Cell</title><addtitle>Cell</addtitle><description>The S. cerevisiae RNA polymerase III (pol III) transcription factor TFIIIB binds to DNA upstream of the transcription start site of the SUP4 tRNATyr gene in a TFIIIC-dependent reaction and to the major 5S rRNA gene in a reaction requiring TFIIIC and TFIIIA. It is shown here that TFIIIB alone correctly positions pol III for repeated cycles of transcription on both genes, with the same efficiency as fully assembled transcription complexes. Thus, TFIIIB is the sole transcription initiation factor of S. cerevisiae pol III; TFIIIC and TFIIIA are assembly factors for TFIIIB. The TFIIIB-dependent binding of pol 111 to the SUP4 tRNA and 5S rRNA genes has been analyzed in binary (protein and DNA only) and precisely arrested ternary (protein, DNA, and RNA) transcription complexes. Pol III unwinds at least 14 by of DNA at the SUP4 transcription start in a temperature-dependent process. The unwound DNA segment moves downstream with nascent RNA as a transcription bubble of approximately the same size.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Deoxyribonuclease I</subject><subject>DNA, Fungal - drug effects</subject><subject>DNA, Fungal - genetics</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - metabolism</subject><subject>Genes, Fungal</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Plasmids</subject><subject>Potassium Permanganate - pharmacology</subject><subject>RNA Polymerase III - metabolism</subject><subject>RNA, Ribosomal, 5S - biosynthesis</subject><subject>RNA, Ribosomal, 5S - genetics</subject><subject>RNA, Transfer, Tyr - genetics</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Transcription Factor TFIIIA</subject><subject>Transcription Factor TFIIIB</subject><subject>Transcription Factors - isolation & purification</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription Factors, TFIII</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-KFDEQh4Mo6zj6Bgo5iCjYayrpdDoXYRxcHVgUdD2H6nQ1G-l_Jj0r8wI-tz07zRz1lIL66kdVPsaeg7gEAcU7IazMysLkr614Y4VRNpMP2AqENVkORj5kqzPymD1J6acQotRaX7ALmYu8AFixP98vuadIdyEFJH5ztdvtPvCQ-HRLfIrYJx_DOIWh56EPU8D7skE_DZGPcRgp8qHh375s-Di0h44iJuJzyFv--za0S-KGY1-fyi3HSBxToq5qD0tSesoeNdgmera8a_bj6uPN9nN2_fXTbru5zvx80ZQhKrAeapCmEI30lTYkRKFAQ41GKyVJ1U0OoCpspEJTkBXkpQTUiqBSa_bqlDuv_mtPaXJdSJ7aFnsa9skZq60yCv4Lgs61hbKYwfwE-jikFKlxYwwdxoMD4Y6e3FGCO0pwVrh7T07OYy-W_H3VUX0eWsTM_ZdLH5PHtplN-JDOWFGqspzvXbP3J4zmT7sLFF3ygXpPdYjkJ1cP4d97_AVtgKza</recordid><startdate>19900126</startdate><enddate>19900126</enddate><creator>Kassavetis, George A.</creator><creator>Braun, Burkhard R.</creator><creator>Nguyen, Lam H.</creator><creator>Peter Geiduschek, E.</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19900126</creationdate><title>S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factors</title><author>Kassavetis, George A. ; Braun, Burkhard R. ; Nguyen, Lam H. ; Peter Geiduschek, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-aa319c1d12760f2cb57e0063151da75332e3df4113baf23a76e90ec221a53e1b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Deoxyribonuclease I</topic><topic>DNA, Fungal - drug effects</topic><topic>DNA, Fungal - genetics</topic><topic>DNA-Directed RNA Polymerases - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal Proteins - metabolism</topic><topic>Genes, Fungal</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Plasmids</topic><topic>Potassium Permanganate - pharmacology</topic><topic>RNA Polymerase III - metabolism</topic><topic>RNA, Ribosomal, 5S - biosynthesis</topic><topic>RNA, Ribosomal, 5S - genetics</topic><topic>RNA, Transfer, Tyr - genetics</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Transcription Factor TFIIIA</topic><topic>Transcription Factor TFIIIB</topic><topic>Transcription Factors - isolation & purification</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription Factors, TFIII</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kassavetis, George A.</creatorcontrib><creatorcontrib>Braun, Burkhard R.</creatorcontrib><creatorcontrib>Nguyen, Lam H.</creatorcontrib><creatorcontrib>Peter Geiduschek, E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kassavetis, George A.</au><au>Braun, Burkhard R.</au><au>Nguyen, Lam H.</au><au>Peter Geiduschek, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factors</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1990-01-26</date><risdate>1990</risdate><volume>60</volume><issue>2</issue><spage>235</spage><epage>245</epage><pages>235-245</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><coden>CELLB5</coden><abstract>The S. cerevisiae RNA polymerase III (pol III) transcription factor TFIIIB binds to DNA upstream of the transcription start site of the SUP4 tRNATyr gene in a TFIIIC-dependent reaction and to the major 5S rRNA gene in a reaction requiring TFIIIC and TFIIIA. It is shown here that TFIIIB alone correctly positions pol III for repeated cycles of transcription on both genes, with the same efficiency as fully assembled transcription complexes. Thus, TFIIIB is the sole transcription initiation factor of S. cerevisiae pol III; TFIIIC and TFIIIA are assembly factors for TFIIIB. The TFIIIB-dependent binding of pol 111 to the SUP4 tRNA and 5S rRNA genes has been analyzed in binary (protein and DNA only) and precisely arrested ternary (protein, DNA, and RNA) transcription complexes. Pol III unwinds at least 14 by of DNA at the SUP4 transcription start in a temperature-dependent process. The unwound DNA segment moves downstream with nascent RNA as a transcription bubble of approximately the same size.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>2404611</pmid><doi>10.1016/0092-8674(90)90739-2</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0092-8674 |
| ispartof | Cell, 1990-01, Vol.60 (2), p.235-245 |
| issn | 0092-8674 1097-4172 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79593731 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Base Sequence Biological and medical sciences Deoxyribonuclease I DNA, Fungal - drug effects DNA, Fungal - genetics DNA-Directed RNA Polymerases - metabolism Fundamental and applied biological sciences. Psychology Fungal Proteins - metabolism Genes, Fungal Molecular and cellular biology Molecular genetics Molecular Sequence Data Multigene Family Plasmids Potassium Permanganate - pharmacology RNA Polymerase III - metabolism RNA, Ribosomal, 5S - biosynthesis RNA, Ribosomal, 5S - genetics RNA, Transfer, Tyr - genetics Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Transcription Factor TFIIIA Transcription Factor TFIIIB Transcription Factors - isolation & purification Transcription Factors - metabolism Transcription Factors, TFIII Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing |
| title | S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-15T12%3A34%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=S.%20cerevisiae%20TFIIIB%20is%20the%20transcription%20initiation%20factor%20proper%20of%20RNA%20polymerase%20III,%20while%20TFIIIA%20and%20TFIIIC%20are%20assembly%20factors&rft.jtitle=Cell&rft.au=Kassavetis,%20George%20A.&rft.date=1990-01-26&rft.volume=60&rft.issue=2&rft.spage=235&rft.epage=245&rft.pages=235-245&rft.issn=0092-8674&rft.eissn=1097-4172&rft.coden=CELLB5&rft_id=info:doi/10.1016/0092-8674(90)90739-2&rft_dat=%3Cproquest_cross%3E15459186%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15459186&rft_id=info:pmid/2404611&rft_els_id=0092867490907392&rfr_iscdi=true |