Preparation and properties of brush-border membrane vesicles from human small intestine
This study describes a simple and rapid method for the preparation of brush-border membrane vesicles from intestinal biopsies. The specific activities of sucrase, amino peptidase N, and alkaline phosphatase in these vesicles were the same as those in vesicles prepared from intestinal segments. The v...
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| Veröffentlicht in: | Gastroenterology (New York, N.Y. 1943) N.Y. 1943), 1990-03, Vol.98 (3), p.676-685 |
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| container_title | Gastroenterology (New York, N.Y. 1943) |
| container_volume | 98 |
| creator | Shirazi-Beechey, S.P. Davies, A.G. Tebbutt, K. Dyer, J. Ellis, A. Taylor, C.J. Fairclough, P. Beechey, R.B. |
| description | This study describes a simple and rapid method for the preparation of brush-border membrane vesicles from intestinal biopsies. The specific activities of sucrase, amino peptidase N, and alkaline phosphatase in these vesicles were the same as those in vesicles prepared from intestinal segments. The vesicles from all the regions of the small intestine can transport d-glucose in an Na+-dependent manner. The rates of transport of d-glucose presented here are far higher than previously reported. The method should have a wide applicability to studies of transport mechanisms and the distribution of transport processes within the intestine. |
| doi_str_mv | 10.1016/0016-5085(90)90288-C |
| format | Article |
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The specific activities of sucrase, amino peptidase N, and alkaline phosphatase in these vesicles were the same as those in vesicles prepared from intestinal segments. The vesicles from all the regions of the small intestine can transport d-glucose in an Na+-dependent manner. The rates of transport of d-glucose presented here are far higher than previously reported. The method should have a wide applicability to studies of transport mechanisms and the distribution of transport processes within the intestine.</description><identifier>ISSN: 0016-5085</identifier><identifier>EISSN: 1528-0012</identifier><identifier>DOI: 10.1016/0016-5085(90)90288-C</identifier><identifier>PMID: 2298371</identifier><identifier>CODEN: GASTAB</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Animals ; Biological and medical sciences ; Biological Transport - physiology ; Biomarkers - analysis ; Biopsy ; Cell Fractionation - methods ; Fundamental and applied biological sciences. Psychology ; Glucose - metabolism ; Humans ; Intestine, Small - enzymology ; Intestine, Small - ultrastructure ; Intestine. Mesentery ; Microvilli - enzymology ; Microvilli - ultrastructure ; Organelles - enzymology ; Organelles - ultrastructure ; Rabbits ; Vertebrates: digestive system</subject><ispartof>Gastroenterology (New York, N.Y. 1943), 1990-03, Vol.98 (3), p.676-685</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-5c864669bfbd437cd3dfebc99a90370b9fb4f4c5f8943e257168b1ddb0b018ea3</citedby><cites>FETCH-LOGICAL-c387t-5c864669bfbd437cd3dfebc99a90370b9fb4f4c5f8943e257168b1ddb0b018ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0016-5085(90)90288-C$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19361862$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2298371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shirazi-Beechey, S.P.</creatorcontrib><creatorcontrib>Davies, A.G.</creatorcontrib><creatorcontrib>Tebbutt, K.</creatorcontrib><creatorcontrib>Dyer, J.</creatorcontrib><creatorcontrib>Ellis, A.</creatorcontrib><creatorcontrib>Taylor, C.J.</creatorcontrib><creatorcontrib>Fairclough, P.</creatorcontrib><creatorcontrib>Beechey, R.B.</creatorcontrib><title>Preparation and properties of brush-border membrane vesicles from human small intestine</title><title>Gastroenterology (New York, N.Y. 1943)</title><addtitle>Gastroenterology</addtitle><description>This study describes a simple and rapid method for the preparation of brush-border membrane vesicles from intestinal biopsies. The specific activities of sucrase, amino peptidase N, and alkaline phosphatase in these vesicles were the same as those in vesicles prepared from intestinal segments. The vesicles from all the regions of the small intestine can transport d-glucose in an Na+-dependent manner. The rates of transport of d-glucose presented here are far higher than previously reported. The method should have a wide applicability to studies of transport mechanisms and the distribution of transport processes within the intestine.</description><subject>Adult</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Transport - physiology</subject><subject>Biomarkers - analysis</subject><subject>Biopsy</subject><subject>Cell Fractionation - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - metabolism</subject><subject>Humans</subject><subject>Intestine, Small - enzymology</subject><subject>Intestine, Small - ultrastructure</subject><subject>Intestine. Mesentery</subject><subject>Microvilli - enzymology</subject><subject>Microvilli - ultrastructure</subject><subject>Organelles - enzymology</subject><subject>Organelles - ultrastructure</subject><subject>Rabbits</subject><subject>Vertebrates: digestive system</subject><issn>0016-5085</issn><issn>1528-0012</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMlKBDEURYMo2g5_oJCNoovSpFJDshGkcIIGXSguQ4YXjNTQJlUN_r1pu9Gdm7zFO-_mchA6puSSElpdkfRkJeHluSAXguScZ80WmtEy51na5dto9ovsof0YPwghgnG6i3bzXHBW0xl6ew6wUEGNfuix6i1ehGEBYfQQ8eCwDlN8z_QQLATcQaeD6gEvIXrTJsKFocPvU6d6HDvVttj3I8TR93CIdpxqIxxt5gF6vbt9aR6y-dP9Y3Mzzwzj9ZiVhldFVQnttC1YbSyzDrQRQgnCaqKF04UrTOm4KBjkZU0rrqm1mmhCOSh2gM7Wuan355T-lp2PBto29RymKGtRipzXdQKLNWjCEGMAJxfBdyp8SUrkyqdcyZIrWVIQ-eNTNunsZJM_6Q7s79FGYNqfbvYqGtW65Mf4-JctWEV5lSfues1BkrH0EGQ0HnoD1gcwo7SD_7_INyb_kvg</recordid><startdate>19900301</startdate><enddate>19900301</enddate><creator>Shirazi-Beechey, S.P.</creator><creator>Davies, A.G.</creator><creator>Tebbutt, K.</creator><creator>Dyer, J.</creator><creator>Ellis, A.</creator><creator>Taylor, C.J.</creator><creator>Fairclough, P.</creator><creator>Beechey, R.B.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900301</creationdate><title>Preparation and properties of brush-border membrane vesicles from human small intestine</title><author>Shirazi-Beechey, S.P. ; Davies, A.G. ; Tebbutt, K. ; Dyer, J. ; Ellis, A. ; Taylor, C.J. ; Fairclough, P. ; Beechey, R.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-5c864669bfbd437cd3dfebc99a90370b9fb4f4c5f8943e257168b1ddb0b018ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - physiology</topic><topic>Biomarkers - analysis</topic><topic>Biopsy</topic><topic>Cell Fractionation - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - metabolism</topic><topic>Humans</topic><topic>Intestine, Small - enzymology</topic><topic>Intestine, Small - ultrastructure</topic><topic>Intestine. Mesentery</topic><topic>Microvilli - enzymology</topic><topic>Microvilli - ultrastructure</topic><topic>Organelles - enzymology</topic><topic>Organelles - ultrastructure</topic><topic>Rabbits</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shirazi-Beechey, S.P.</creatorcontrib><creatorcontrib>Davies, A.G.</creatorcontrib><creatorcontrib>Tebbutt, K.</creatorcontrib><creatorcontrib>Dyer, J.</creatorcontrib><creatorcontrib>Ellis, A.</creatorcontrib><creatorcontrib>Taylor, C.J.</creatorcontrib><creatorcontrib>Fairclough, P.</creatorcontrib><creatorcontrib>Beechey, R.B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shirazi-Beechey, S.P.</au><au>Davies, A.G.</au><au>Tebbutt, K.</au><au>Dyer, J.</au><au>Ellis, A.</au><au>Taylor, C.J.</au><au>Fairclough, P.</au><au>Beechey, R.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and properties of brush-border membrane vesicles from human small intestine</atitle><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle><addtitle>Gastroenterology</addtitle><date>1990-03-01</date><risdate>1990</risdate><volume>98</volume><issue>3</issue><spage>676</spage><epage>685</epage><pages>676-685</pages><issn>0016-5085</issn><eissn>1528-0012</eissn><coden>GASTAB</coden><abstract>This study describes a simple and rapid method for the preparation of brush-border membrane vesicles from intestinal biopsies. The specific activities of sucrase, amino peptidase N, and alkaline phosphatase in these vesicles were the same as those in vesicles prepared from intestinal segments. The vesicles from all the regions of the small intestine can transport d-glucose in an Na+-dependent manner. The rates of transport of d-glucose presented here are far higher than previously reported. The method should have a wide applicability to studies of transport mechanisms and the distribution of transport processes within the intestine.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>2298371</pmid><doi>10.1016/0016-5085(90)90288-C</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0016-5085 |
| ispartof | Gastroenterology (New York, N.Y. 1943), 1990-03, Vol.98 (3), p.676-685 |
| issn | 0016-5085 1528-0012 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); Alma/SFX Local Collection |
| subjects | Adult Animals Biological and medical sciences Biological Transport - physiology Biomarkers - analysis Biopsy Cell Fractionation - methods Fundamental and applied biological sciences. Psychology Glucose - metabolism Humans Intestine, Small - enzymology Intestine, Small - ultrastructure Intestine. Mesentery Microvilli - enzymology Microvilli - ultrastructure Organelles - enzymology Organelles - ultrastructure Rabbits Vertebrates: digestive system |
| title | Preparation and properties of brush-border membrane vesicles from human small intestine |
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