Cloning and nucleotide sequence of the alpha-galactosidase cDNA from Cyamopsis tetragonoloba (guar)

Polyadenylated mRNA was purified from the aleurone cells of Cyamopsis tetragonoloba (guar) seeds germinated for 18 h and used for the construction of a cDNA library. Clones with the alpha-galactosidase encoding gene were identified using oligo-nucleotide mixed probes based on the NH2 terminal amino...

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Veröffentlicht in:	Plant molecular biology 1989-11, Vol.13 (5), p.541-550
Hauptverfasser:	Overbeeke, N. (Unilever Research Lab., Vlaardingen (Netherlands)), Fellinger, A.J, Toonen, M.Y, Wassenaar, D. van, Verrips, C.T
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN alpha -galactosidase Amino Acid Sequence Base Sequence Biological and medical sciences Biotechnology CLONACION CLONAGE CLONING Cloning, Molecular CODE GENETIQUE CODIGO GENETICO CYAMOPSIS PSORALIOIDES DNA DNA - genetics Enzyme Precursors - genetics Fundamental and applied biological sciences. Psychology GALACTOSIDASA GALACTOSIDASE GALACTOSIDASES Galactosidases - genetics genes Genes. Genome GENETIC CODE Genetic engineering Genetic technics Humans Methods. Procedures. Technologies Molecular and cellular biology Molecular cloning Molecular genetics Molecular Sequence Data Plants - enzymology Plants - genetics Poly A - genetics Restriction Mapping RNA, Messenger - genetics Sequence Homology, Nucleic Acid Species Specificity
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