Cloning and expression of the chloroplast-encoded rbcL and rbcS genes from the marine diatom Cylindrotheca sp. strain N1
Both the rbcL and rbcS genes, encoding the large and small subunits, respectively, of ribulose 1,5-bisphosphate carboxylase/oxygenase, have been found to be encoded by chloroplast DNA in the marine diatom Cylindrotheca sp. N1. The rbcS gene in this diatom was found to be adjacent to the rbcL gene by...
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| Veröffentlicht in: | Plant molecular biology 1989-07, Vol.13 (1), p.69-79 |
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| creator | Hwang, S.R Tabita, F.R |
| description | Both the rbcL and rbcS genes, encoding the large and small subunits, respectively, of ribulose 1,5-bisphosphate carboxylase/oxygenase, have been found to be encoded by chloroplast DNA in the marine diatom Cylindrotheca sp. N1. The rbcS gene in this diatom was found to be adjacent to the rbcL gene by a combination of: (i) Southern-blotting analyses, using heterologous probes; (ii) examination of recombinant proteins synthesized in Escherichia coli, directed by cloned rbcL/rbcS genes; and (iii) synthesis of enzymatically active heterologous Rubisco protein in vivo by recombinant DNA procedures using large subunits of Anacystis nidulans and small subunits of Cylindrotheca sp. N1. It appears that two copies of rbcL and rbcS genes are encoded by the chloroplast DNA of this diatom. |
| doi_str_mv | 10.1007/BF00027336 |
| format | Article |
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N1. The rbcS gene in this diatom was found to be adjacent to the rbcL gene by a combination of: (i) Southern-blotting analyses, using heterologous probes; (ii) examination of recombinant proteins synthesized in Escherichia coli, directed by cloned rbcL/rbcS genes; and (iii) synthesis of enzymatically active heterologous Rubisco protein in vivo by recombinant DNA procedures using large subunits of Anacystis nidulans and small subunits of Cylindrotheca sp. N1. It appears that two copies of rbcL and rbcS genes are encoded by the chloroplast DNA of this diatom.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/BF00027336</identifier><identifier>PMID: 2562761</identifier><identifier>CODEN: PMBIDB</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Bacillariophyta ; Biological and medical sciences ; Biotechnology ; Blotting, Southern ; chloroplast DNA ; Chloroplasts - enzymology ; chromosome mapping ; cloning ; Cloning, Molecular ; Cylindrotheca ; Diatoms - enzymology ; Diatoms - genetics ; DNA - metabolism ; enzyme activity ; Escherichia coli ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Gene expression ; gene splicing ; Genetic engineering ; Genetic technics ; genome ; genomics ; Kinetics ; Macromolecular Substances ; Marine ; Methods. Procedures. Technologies ; Molecular and cellular biology ; Molecular cloning ; Molecular genetics ; Molecular Weight ; multiple genes ; Plasmids ; recombinant DNA ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - isolation & purification ; Recombinant Proteins - metabolism ; Restriction Mapping ; ribulose-bisphosphate carboxylase ; Ribulose-Bisphosphate Carboxylase - biosynthesis ; Ribulose-Bisphosphate Carboxylase - genetics ; Ribulose-Bisphosphate Carboxylase - metabolism ; Synechococcus</subject><ispartof>Plant molecular biology, 1989-07, Vol.13 (1), p.69-79</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-262c6252d5cfe63cb09ce0df7120aad4fa64a06c46493463951b7340ad657aa23</citedby><cites>FETCH-LOGICAL-c366t-262c6252d5cfe63cb09ce0df7120aad4fa64a06c46493463951b7340ad657aa23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6680937$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2562761$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hwang, S.R</creatorcontrib><creatorcontrib>Tabita, F.R</creatorcontrib><title>Cloning and expression of the chloroplast-encoded rbcL and rbcS genes from the marine diatom Cylindrotheca sp. strain N1</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>Both the rbcL and rbcS genes, encoding the large and small subunits, respectively, of ribulose 1,5-bisphosphate carboxylase/oxygenase, have been found to be encoded by chloroplast DNA in the marine diatom Cylindrotheca sp. N1. The rbcS gene in this diatom was found to be adjacent to the rbcL gene by a combination of: (i) Southern-blotting analyses, using heterologous probes; (ii) examination of recombinant proteins synthesized in Escherichia coli, directed by cloned rbcL/rbcS genes; and (iii) synthesis of enzymatically active heterologous Rubisco protein in vivo by recombinant DNA procedures using large subunits of Anacystis nidulans and small subunits of Cylindrotheca sp. N1. It appears that two copies of rbcL and rbcS genes are encoded by the chloroplast DNA of this diatom.</description><subject>Bacillariophyta</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blotting, Southern</subject><subject>chloroplast DNA</subject><subject>Chloroplasts - enzymology</subject><subject>chromosome mapping</subject><subject>cloning</subject><subject>Cloning, Molecular</subject><subject>Cylindrotheca</subject><subject>Diatoms - enzymology</subject><subject>Diatoms - genetics</subject><subject>DNA - metabolism</subject><subject>enzyme activity</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>gene splicing</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>genome</subject><subject>genomics</subject><subject>Kinetics</subject><subject>Macromolecular Substances</subject><subject>Marine</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular and cellular biology</subject><subject>Molecular cloning</subject><subject>Molecular genetics</subject><subject>Molecular Weight</subject><subject>multiple genes</subject><subject>Plasmids</subject><subject>recombinant DNA</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - isolation & purification</subject><subject>Recombinant Proteins - metabolism</subject><subject>Restriction Mapping</subject><subject>ribulose-bisphosphate carboxylase</subject><subject>Ribulose-Bisphosphate Carboxylase - biosynthesis</subject><subject>Ribulose-Bisphosphate Carboxylase - genetics</subject><subject>Ribulose-Bisphosphate Carboxylase - metabolism</subject><subject>Synechococcus</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U1PGzEQBmCrKqIp7YU7wgfUQ6WFsb22s0eISosUwYFyXk38EVxt7K29keDf15AIjj3Z8jwzkucl5JjBOQPQF1fXAMC1EOoDmTGpRSOBzz-SGTClm7Zl_BP5XMofgMqFOiSHXCquFZuRp8WQYohritFS9zRmV0pIkSZPp0dHzeOQchoHLFPjoknWWZpXZvnK6-Werl10hfqcNq8NG8whOmoDTvVl8TyEaHOqFYO0jOe0TBlDpLfsCznwOBT3dX8ekYfrH78Xv5rl3c-bxeWyMUKpqeGKG8Ult9J4p4RZQWccWK8ZB0TbelQtgjKtajvRKtFJttKiBbRKakQujsi33dwxp79bV6Z-E4pxw4DRpW3pdSe1ro3_hUwKOa-rrPD7DpqcSsnO92MO9d_PPYP-JY_-PY-KT_ZTt6uNs290H0Ctn-3rWAwOPmM0obwxpebQCV3Z6Y55TD2ucyUP9xyYAKZFVxck_gHeI5pq</recordid><startdate>19890701</startdate><enddate>19890701</enddate><creator>Hwang, S.R</creator><creator>Tabita, F.R</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19890701</creationdate><title>Cloning and expression of the chloroplast-encoded rbcL and rbcS genes from the marine diatom Cylindrotheca sp. strain N1</title><author>Hwang, S.R ; Tabita, F.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-262c6252d5cfe63cb09ce0df7120aad4fa64a06c46493463951b7340ad657aa23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Bacillariophyta</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blotting, Southern</topic><topic>chloroplast DNA</topic><topic>Chloroplasts - enzymology</topic><topic>chromosome mapping</topic><topic>cloning</topic><topic>Cloning, Molecular</topic><topic>Cylindrotheca</topic><topic>Diatoms - enzymology</topic><topic>Diatoms - genetics</topic><topic>DNA - metabolism</topic><topic>enzyme activity</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>gene splicing</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>genome</topic><topic>genomics</topic><topic>Kinetics</topic><topic>Macromolecular Substances</topic><topic>Marine</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular and cellular biology</topic><topic>Molecular cloning</topic><topic>Molecular genetics</topic><topic>Molecular Weight</topic><topic>multiple genes</topic><topic>Plasmids</topic><topic>recombinant DNA</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Recombinant Proteins - isolation & purification</topic><topic>Recombinant Proteins - metabolism</topic><topic>Restriction Mapping</topic><topic>ribulose-bisphosphate carboxylase</topic><topic>Ribulose-Bisphosphate Carboxylase - biosynthesis</topic><topic>Ribulose-Bisphosphate Carboxylase - genetics</topic><topic>Ribulose-Bisphosphate Carboxylase - metabolism</topic><topic>Synechococcus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hwang, S.R</creatorcontrib><creatorcontrib>Tabita, F.R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hwang, S.R</au><au>Tabita, F.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and expression of the chloroplast-encoded rbcL and rbcS genes from the marine diatom Cylindrotheca sp. strain N1</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1989-07-01</date><risdate>1989</risdate><volume>13</volume><issue>1</issue><spage>69</spage><epage>79</epage><pages>69-79</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><coden>PMBIDB</coden><abstract>Both the rbcL and rbcS genes, encoding the large and small subunits, respectively, of ribulose 1,5-bisphosphate carboxylase/oxygenase, have been found to be encoded by chloroplast DNA in the marine diatom Cylindrotheca sp. N1. The rbcS gene in this diatom was found to be adjacent to the rbcL gene by a combination of: (i) Southern-blotting analyses, using heterologous probes; (ii) examination of recombinant proteins synthesized in Escherichia coli, directed by cloned rbcL/rbcS genes; and (iii) synthesis of enzymatically active heterologous Rubisco protein in vivo by recombinant DNA procedures using large subunits of Anacystis nidulans and small subunits of Cylindrotheca sp. N1. It appears that two copies of rbcL and rbcS genes are encoded by the chloroplast DNA of this diatom.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>2562761</pmid><doi>10.1007/BF00027336</doi><tpages>11</tpages></addata></record> |
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| subjects | Bacillariophyta Biological and medical sciences Biotechnology Blotting, Southern chloroplast DNA Chloroplasts - enzymology chromosome mapping cloning Cloning, Molecular Cylindrotheca Diatoms - enzymology Diatoms - genetics DNA - metabolism enzyme activity Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene expression gene splicing Genetic engineering Genetic technics genome genomics Kinetics Macromolecular Substances Marine Methods. Procedures. Technologies Molecular and cellular biology Molecular cloning Molecular genetics Molecular Weight multiple genes Plasmids recombinant DNA Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Restriction Mapping ribulose-bisphosphate carboxylase Ribulose-Bisphosphate Carboxylase - biosynthesis Ribulose-Bisphosphate Carboxylase - genetics Ribulose-Bisphosphate Carboxylase - metabolism Synechococcus |
| title | Cloning and expression of the chloroplast-encoded rbcL and rbcS genes from the marine diatom Cylindrotheca sp. strain N1 |
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