Vaccination against Babesia bovis: T cells from protected and unprotected animals show different cytokine profiles
Vaccination of cattle against the haemoprotozoun parasite, Babesia bovis, with the recombinant antigen 11C5 resulted in 9 of 15 cattle being protected against challenge infection. The cellular immune responses of protected and unprotected cattle were compared in order to identify differences in resp...
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| Veröffentlicht in: | International journal for parasitology 1997-12, Vol.27 (12), p.1537-1545 |
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| container_title | International journal for parasitology |
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| creator | East, I.J. Zakrzewski, H. Gale, K.R. Leatch, G. Dimmock, C.M. Thomas, M.B. Waltisbuhl, D.J. |
| description | Vaccination of cattle against the haemoprotozoun parasite,
Babesia bovis, with the recombinant antigen 11C5 resulted in 9 of 15 cattle being protected against challenge infection. The cellular immune responses of protected and unprotected cattle were compared in order to identify differences in response. No differences were observed in the pattern of change in various blood leukocyte populations throughout challenge infection. FACScan analysis revealed an increase in the proportion of cells bearing the CD2 marker in both protected and unprotected cattle over the course of infection. There were no observable differences in the frequency of various cell-surface markers between the unprotected and protected cattle. During the period of patent parasitaemia,
in vitro cultures of peripheral blood mononuclear cells (PBMC) from protected cattle produced significantly more TNF-α (
P < 0.05) than cultures from unprotected cattle. TNF-α concentrations remained at pre-challenge levels until day 10, when levels in the unvaccinated control and vaccinated/unprotected animals dropped. By peak parasitaemia, TNF-α production
in vitro was siguificantly greater (
P < 0.05) in cultures of PBMCs from protected cattle. Interferon production showed an initial peak at day 5 in all cattle, followed by a decrease and a second peak at days 10–13 in protected cattle only, which coincided with resolution of the infection. |
| doi_str_mv | 10.1016/S0020-7519(97)00141-0 |
| format | Article |
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Babesia bovis, with the recombinant antigen 11C5 resulted in 9 of 15 cattle being protected against challenge infection. The cellular immune responses of protected and unprotected cattle were compared in order to identify differences in response. No differences were observed in the pattern of change in various blood leukocyte populations throughout challenge infection. FACScan analysis revealed an increase in the proportion of cells bearing the CD2 marker in both protected and unprotected cattle over the course of infection. There were no observable differences in the frequency of various cell-surface markers between the unprotected and protected cattle. During the period of patent parasitaemia,
in vitro cultures of peripheral blood mononuclear cells (PBMC) from protected cattle produced significantly more TNF-α (
P < 0.05) than cultures from unprotected cattle. TNF-α concentrations remained at pre-challenge levels until day 10, when levels in the unvaccinated control and vaccinated/unprotected animals dropped. By peak parasitaemia, TNF-α production
in vitro was siguificantly greater (
P < 0.05) in cultures of PBMCs from protected cattle. Interferon production showed an initial peak at day 5 in all cattle, followed by a decrease and a second peak at days 10–13 in protected cattle only, which coincided with resolution of the infection.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/S0020-7519(97)00141-0</identifier><identifier>PMID: 9467739</identifier><identifier>CODEN: IJPYBT</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Babesia bovis ; Babesia bovis - immunology ; Babesiosis - immunology ; Biological and medical sciences ; Cattle ; Cattle Diseases - immunology ; Cytokines - biosynthesis ; Experimental protozoal diseases and models ; immunisation ; Immunity, Cellular ; Infectious diseases ; interferon (IFN) ; Interferon-gamma - biosynthesis ; Interleukin-2 - biosynthesis ; Leukocytes, Mononuclear - immunology ; Lymphocyte Activation ; Male ; Medical sciences ; Parasitemia ; Parasitic diseases ; protection ; Protozoal diseases ; Protozoan Vaccines - immunology ; T-Lymphocytes - immunology ; Tumor Necrosis Factor-alpha - biosynthesis ; tumour necrosis factor (TNF) ; Vaccination - veterinary ; Vaccines, Synthetic - immunology</subject><ispartof>International journal for parasitology, 1997-12, Vol.27 (12), p.1537-1545</ispartof><rights>1997</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-a30794a969d6779d09f103eb019501d1a140c9c87088d6a181bcd20c908c5b573</citedby><cites>FETCH-LOGICAL-c420t-a30794a969d6779d09f103eb019501d1a140c9c87088d6a181bcd20c908c5b573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0020751997001410$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2128475$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9467739$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>East, I.J.</creatorcontrib><creatorcontrib>Zakrzewski, H.</creatorcontrib><creatorcontrib>Gale, K.R.</creatorcontrib><creatorcontrib>Leatch, G.</creatorcontrib><creatorcontrib>Dimmock, C.M.</creatorcontrib><creatorcontrib>Thomas, M.B.</creatorcontrib><creatorcontrib>Waltisbuhl, D.J.</creatorcontrib><title>Vaccination against Babesia bovis: T cells from protected and unprotected animals show different cytokine profiles</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>Vaccination of cattle against the haemoprotozoun parasite,
Babesia bovis, with the recombinant antigen 11C5 resulted in 9 of 15 cattle being protected against challenge infection. The cellular immune responses of protected and unprotected cattle were compared in order to identify differences in response. No differences were observed in the pattern of change in various blood leukocyte populations throughout challenge infection. FACScan analysis revealed an increase in the proportion of cells bearing the CD2 marker in both protected and unprotected cattle over the course of infection. There were no observable differences in the frequency of various cell-surface markers between the unprotected and protected cattle. During the period of patent parasitaemia,
in vitro cultures of peripheral blood mononuclear cells (PBMC) from protected cattle produced significantly more TNF-α (
P < 0.05) than cultures from unprotected cattle. TNF-α concentrations remained at pre-challenge levels until day 10, when levels in the unvaccinated control and vaccinated/unprotected animals dropped. By peak parasitaemia, TNF-α production
in vitro was siguificantly greater (
P < 0.05) in cultures of PBMCs from protected cattle. Interferon production showed an initial peak at day 5 in all cattle, followed by a decrease and a second peak at days 10–13 in protected cattle only, which coincided with resolution of the infection.</description><subject>Animals</subject><subject>Babesia bovis</subject><subject>Babesia bovis - immunology</subject><subject>Babesiosis - immunology</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cattle Diseases - immunology</subject><subject>Cytokines - biosynthesis</subject><subject>Experimental protozoal diseases and models</subject><subject>immunisation</subject><subject>Immunity, Cellular</subject><subject>Infectious diseases</subject><subject>interferon (IFN)</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interleukin-2 - biosynthesis</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Lymphocyte Activation</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Parasitemia</subject><subject>Parasitic diseases</subject><subject>protection</subject><subject>Protozoal diseases</subject><subject>Protozoan Vaccines - immunology</subject><subject>T-Lymphocytes - immunology</subject><subject>Tumor Necrosis Factor-alpha - biosynthesis</subject><subject>tumour necrosis factor (TNF)</subject><subject>Vaccination - veterinary</subject><subject>Vaccines, Synthetic - immunology</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi1EVZbCT6jkA0JwSJlJ7DjmgqACWqkSBwpXy7EnYNi1i50t6r_H6a5W3HqyxvO88_UydopwhoD9m68ALTRKon6l1WsAFNjAI7bCQekGsJOP2eqAPGFPS_lVIdkJccyOteiV6vSK5e_WuRDtHFLk9ocNscz8gx2pBMvHdBvKW37NHa3XhU85bfhNTjO5mTy30fNt_D8OG1ux8jP95T5ME2WKM3d3c_odIi3KKaypPGNHU-Xo-f49Yd8-fbw-v2iuvny-PH9_1TjRwtzYDpQWVvfa11m1Bz0hdDQCagno0aIAp92gYBh8b3HA0fm2fsHg5ChVd8Je7urWxn-2VGazCWXZxEZK22KUllIKKR4EsW8HEN1SUe5Al1MpmSZzk-vO-c4gmMUUc2-KWS5utDL3phioutN9g-24IX9Q7V2o-Rf7vC3OrqdsowvlgLXYDkLJir3bYVSvdhsom-ICRUc-5GqB8Sk8MMg_mBCo-g</recordid><startdate>19971201</startdate><enddate>19971201</enddate><creator>East, I.J.</creator><creator>Zakrzewski, H.</creator><creator>Gale, K.R.</creator><creator>Leatch, G.</creator><creator>Dimmock, C.M.</creator><creator>Thomas, M.B.</creator><creator>Waltisbuhl, D.J.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19971201</creationdate><title>Vaccination against Babesia bovis: T cells from protected and unprotected animals show different cytokine profiles</title><author>East, I.J. ; Zakrzewski, H. ; Gale, K.R. ; Leatch, G. ; Dimmock, C.M. ; Thomas, M.B. ; Waltisbuhl, D.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-a30794a969d6779d09f103eb019501d1a140c9c87088d6a181bcd20c908c5b573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Babesia bovis</topic><topic>Babesia bovis - immunology</topic><topic>Babesiosis - immunology</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cattle Diseases - immunology</topic><topic>Cytokines - biosynthesis</topic><topic>Experimental protozoal diseases and models</topic><topic>immunisation</topic><topic>Immunity, Cellular</topic><topic>Infectious diseases</topic><topic>interferon (IFN)</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Interleukin-2 - biosynthesis</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Lymphocyte Activation</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Parasitemia</topic><topic>Parasitic diseases</topic><topic>protection</topic><topic>Protozoal diseases</topic><topic>Protozoan Vaccines - immunology</topic><topic>T-Lymphocytes - immunology</topic><topic>Tumor Necrosis Factor-alpha - biosynthesis</topic><topic>tumour necrosis factor (TNF)</topic><topic>Vaccination - veterinary</topic><topic>Vaccines, Synthetic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>East, I.J.</creatorcontrib><creatorcontrib>Zakrzewski, H.</creatorcontrib><creatorcontrib>Gale, K.R.</creatorcontrib><creatorcontrib>Leatch, G.</creatorcontrib><creatorcontrib>Dimmock, C.M.</creatorcontrib><creatorcontrib>Thomas, M.B.</creatorcontrib><creatorcontrib>Waltisbuhl, D.J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>East, I.J.</au><au>Zakrzewski, H.</au><au>Gale, K.R.</au><au>Leatch, G.</au><au>Dimmock, C.M.</au><au>Thomas, M.B.</au><au>Waltisbuhl, D.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vaccination against Babesia bovis: T cells from protected and unprotected animals show different cytokine profiles</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>1997-12-01</date><risdate>1997</risdate><volume>27</volume><issue>12</issue><spage>1537</spage><epage>1545</epage><pages>1537-1545</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><coden>IJPYBT</coden><abstract>Vaccination of cattle against the haemoprotozoun parasite,
Babesia bovis, with the recombinant antigen 11C5 resulted in 9 of 15 cattle being protected against challenge infection. The cellular immune responses of protected and unprotected cattle were compared in order to identify differences in response. No differences were observed in the pattern of change in various blood leukocyte populations throughout challenge infection. FACScan analysis revealed an increase in the proportion of cells bearing the CD2 marker in both protected and unprotected cattle over the course of infection. There were no observable differences in the frequency of various cell-surface markers between the unprotected and protected cattle. During the period of patent parasitaemia,
in vitro cultures of peripheral blood mononuclear cells (PBMC) from protected cattle produced significantly more TNF-α (
P < 0.05) than cultures from unprotected cattle. TNF-α concentrations remained at pre-challenge levels until day 10, when levels in the unvaccinated control and vaccinated/unprotected animals dropped. By peak parasitaemia, TNF-α production
in vitro was siguificantly greater (
P < 0.05) in cultures of PBMCs from protected cattle. Interferon production showed an initial peak at day 5 in all cattle, followed by a decrease and a second peak at days 10–13 in protected cattle only, which coincided with resolution of the infection.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>9467739</pmid><doi>10.1016/S0020-7519(97)00141-0</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0020-7519 |
| ispartof | International journal for parasitology, 1997-12, Vol.27 (12), p.1537-1545 |
| issn | 0020-7519 1879-0135 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79555454 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Babesia bovis Babesia bovis - immunology Babesiosis - immunology Biological and medical sciences Cattle Cattle Diseases - immunology Cytokines - biosynthesis Experimental protozoal diseases and models immunisation Immunity, Cellular Infectious diseases interferon (IFN) Interferon-gamma - biosynthesis Interleukin-2 - biosynthesis Leukocytes, Mononuclear - immunology Lymphocyte Activation Male Medical sciences Parasitemia Parasitic diseases protection Protozoal diseases Protozoan Vaccines - immunology T-Lymphocytes - immunology Tumor Necrosis Factor-alpha - biosynthesis tumour necrosis factor (TNF) Vaccination - veterinary Vaccines, Synthetic - immunology |
| title | Vaccination against Babesia bovis: T cells from protected and unprotected animals show different cytokine profiles |
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